ABSTRACT
Bone scintigraphy in 26 patients with adult T-cell leukemia-lymphoma (ATLL) was evaluated. Abnormally high accumulations were observed in 7 of 26 patients (skull, 5; vertebra, 1; rib, 6; bony pelvis, 1; scapula, 2; lower extremities, 1). Serum calcium levels were significantly elevated in patients with abnormally high accumulations on bone scintigraphy. Survival rates of the patients with positive results on bone scintigraphy were significantly lower than were those with negative results on bone scintigraphy (P < 0.05). Survival rates of patients with high serum calcium levels, high WBC counts, and high serum lactate dehydrogenase (LDH) levels were also significantly lower than were those of the negative groups, in this order of significance. Bone scintigraphy was useful for detecting bone marrow involvement in ATLL and it can be one of the better indicators of the prognoses of patients with ATLL.
Subject(s)
Bone and Bones/diagnostic imaging , Leukemia-Lymphoma, Adult T-Cell/diagnostic imaging , Leukemia-Lymphoma, Adult T-Cell/mortality , Technetium Tc 99m Medronate , Calcium/blood , Female , Humans , L-Lactate Dehydrogenase/blood , Leukemia-Lymphoma, Adult T-Cell/blood , Leukocyte Count , Male , Middle Aged , Prognosis , Radionuclide Imaging , Survival RateABSTRACT
Human skin is known to contain protein-bound citrulline. This is the product of enzymatic deimination of arginine residues catalyzed by peptidylarginine deiminase. We probed frozen sections of human skin with a rabbit antiserum raised to rat skeletal muscle peptidylarginine deiminase using the avidin-biotin-peroxidase complex technique. This led us to interesting findings. No staining was observed in epidermis, inner root sheaths of hair follicles, sebaceous glands, and hair erector muscle. However, we noticed specific staining of the cytoplasm of secretory and myoepithelial cells of both eccrine and apocrine sweat glands. The procedure also stained neoplastic cells present in specimens dissected from extramammary Paget's disease. The data mean that peptidylarginine deiminase may be used as a new marker in the classification of skin neoplasms showing sweat gland differentiation. Possible localization of multiple types of peptidylarginine deiminases in human skin is discussed.
Subject(s)
Biomarkers, Tumor/analysis , Hydrolases/analysis , Skin Neoplasms/enzymology , Sweat Glands/enzymology , Antigens, Differentiation/analysis , Antigens, Surface/analysis , Apocrine Glands/enzymology , Apocrine Glands/pathology , Citrulline/analysis , Cytoplasm/enzymology , Eccrine Glands/enzymology , Eccrine Glands/pathology , Humans , Immunoenzyme Techniques , Paget Disease, Extramammary/enzymology , Paget Disease, Extramammary/pathology , Protein-Arginine Deiminase Type 4 , Protein-Arginine Deiminases , Skin Neoplasms/pathology , Staining and Labeling , Sweat Glands/pathologyABSTRACT
Using 125I-EGF the distribution of EGF receptors in psoriatic epidermis was autoradiographically examined. Binding sites of 125I-EGF (20 ng/ml) were mainly located at basal and suprabasal cells in normal and uninvolved psoriatic epidermis, whereas they were located at spinous cells as well as at basal and suprabasal cells in affected psoriatic epidermis. Binding of 125I-EGF (1 ng/ml), on the other hand, was observed in normal and uninvolved psoriatic epidermis but not in affected psoriatic epidermis. The majority of EGF receptors in affected psoriatic epidermis were featured by low affinity for EGF.
Subject(s)
Epidermis/metabolism , ErbB Receptors/metabolism , Psoriasis/metabolism , Autoradiography , Epidermal Growth Factor/metabolism , Humans , Keratinocytes/metabolismABSTRACT
Dispersed hair follicle cells from plucked out hairs have been successfully grown on collagen type IV without a biological feeder layer. The optimal calcium concentration for the culture of these cells was also studied and decided to be 0.3-0.5 mM. In this calcium range and on collagen type IV, colony formation and colony growth were best and big colonies with a paving stone-like cell arrangement were formed. The availability of such cultured cells for the treatment of missing skin is proposed instead of cultured epidermal keratinocytes which have been commonly used for wound dressing.
Subject(s)
Hair/cytology , Calcium/pharmacology , Cell Differentiation , Cell Division , Cells, Cultured , Collagen , HumansSubject(s)
Fibrinolysis , Furocoumarins/pharmacology , Skin/metabolism , Ultraviolet Rays , Humans , Skin/drug effects , Skin/radiation effects , Time FactorsABSTRACT
One of our co-workers was sensitized with a 1% DNCB solution in acetone. Biopsies were obtained from skin lesions 1, 2, 4, 8, 12, 24, 30, 36 and 48 hours after a challenge with 0.1% DNCB solution. In the upper dermis, a rapid decrease of tissue fibrinolytic activity was found which disappeared after 30 hours. In the lower dermis, biphasic changes were observed. Tissue fibrinolytic activity increased one hour and 12-36 hours after challenge, whereas at 48 hours, when erythema was most intensive, it decreased in comparison to the normal activity. Erythema and histological changes of the epidermis and the dermis appeared after approximately 12 hours. As for lymphocytes, T-cells were seen at first. Furthermore, B-cells were noted after approximately 30 hours when marked infiltration began to appear.
