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Fluorescence amplified detection of proteases by the catalytic activation of a semisynthetic sensor.
Chem Commun (Camb)
; 49(55): 6212-4, 2013 Jul 14.
Article
in English
| MEDLINE
| ID: mdl-23736313
ABSTRACT
A general approach to mimic the sensing scheme of allosteric enzymes is developed. Through the covalent labeling of a sulfonamide inhibitor to the enzyme HCAII via SNAP-tag protein, the enzyme is rendered inactive. Catalytic activation is triggered only when a protease is present to cleave the recognized peptide sequence.