ABSTRACT
The experimental model of synchronous multiple primary malignant tumors (MPMT) was created. B16/F10 melanoma (0.5 ml of suspension diluted 1:20 in saline) and sarcoma 45 (0.5 million tumor cells in 0.5 ml saline) were simultaneously subcutaneously inoculated to male BALB/c nude mice. In the model of synchronous MPMT, the tumors appeared faster by 2.4 times and had greater volumes: melanoma by 2.2 times and sarcoma by 3.2 times; melanoma metastasized into sarcoma in 71.4% cases; the survival of mice with MPMT was lower. The altered dynamics of malignant growth in the MPMT model is based on the mutual influence of tumors, which results in the exchange of "structural information".
Subject(s)
Lung Neoplasms/pathology , Melanoma, Experimental/pathology , Neoplasm Transplantation/methods , Neoplasms, Multiple Primary/pathology , Primary Immunodeficiency Diseases/pathology , Skin Neoplasms/pathology , Animals , Cell Line, Tumor , Lung Neoplasms/genetics , Lung Neoplasms/immunology , Male , Melanoma, Experimental/genetics , Melanoma, Experimental/immunology , Mice , Mice, Inbred BALB C , Mice, Nude , Neoplasms, Multiple Primary/genetics , Neoplasms, Multiple Primary/immunology , Primary Immunodeficiency Diseases/genetics , Primary Immunodeficiency Diseases/immunology , Rats , Skin Neoplasms/genetics , Skin Neoplasms/immunologyABSTRACT
Experimental study of extracorporeal exposure of autoblood to red monochromatic photodiode radiation for improving antitumor efficiency of cyclophosphamide injected with autoblood was carried out on the model of sarcoma 45 cells ectopically transplanted to the lungs. Co-incubation of irradiated autoblood with cyclophosphamide and reinfusion of the blood to animals increased the antitumor efficiency of chemotherapy in animals with tumors.
Subject(s)
Blood Transfusion, Autologous/methods , Cyclophosphamide/therapeutic use , Lasers, Semiconductor/therapeutic use , Photopheresis/methods , Sarcoma/therapy , Animals , Antineoplastic Agents/administration & dosage , Antineoplastic Agents/therapeutic use , Combined Modality Therapy , Cyclophosphamide/administration & dosage , Infusions, Intravenous , Low-Level Light Therapy , Lung/drug effects , Lung/pathology , Male , Neoplasm Transplantation , Rats , Sarcoma/pathology , Tumor Burden/drug effectsABSTRACT
Effects of exposure to low-intensity monochromatic red spectrum radiation in different modes were studied on L929 skin fibroblast culture. Radiometric and cytological study of cell culture before and after irradiation showed that monochromatic low-intensity radiation stimulated skin fibroblasts in L929 culture under certain conditions.
Subject(s)
Cell Proliferation/radiation effects , Fibroblasts/physiology , Light , Skin/cytology , Animals , Case-Control Studies , Cell Culture Techniques , Cell Differentiation/radiation effects , Cell Shape/radiation effects , Fibroblasts/radiation effects , MiceABSTRACT
Effects of various physical factors of electromagnetic nature on the synthetic and phagocytic activities of neutrophils were studied in vitro on the blood of patients with breast cancer. We found that alternating magnetic field, low-intensity laser, photodiod radiation, and their combinations induced mobilization of blood neutrophil function.
Subject(s)
Breast Neoplasms/immunology , Neutrophils/radiation effects , Case-Control Studies , Electromagnetic Fields , Female , Humans , Neutrophils/physiology , Phagocytosis/radiation effects , Radiation , Spectrometry, FluorescenceABSTRACT
Exposure of rats with Pliss lymphosarcoma to incoherent red light according to certain protocols creates conditions preventing suppression of antitumor resistance, reducing the toxic effect of cyclophosphamide, and potentiating its antitumor effect.
