[The use of discriminant analysis of serum creatine kinase levels for detection of heterozygote carriers of Duchenne muscular dystrophy]. / Ispol'zovanie metoda diskriminantnogo analiza urovnei syvorotochnoi kreatinkinazy v ustanovlenii geterozigotnogo nositel'stva myshechnoi distrofii Diushenna.

The possibility of using discriminant analysis of creatine kinase levels for detection of Duchenne muscular dystrophy heterozygote carrier was studied. Application of combined genealogical and biochemical information enhanced detection of heterozygotes. It is proposed to apply this method for medical and genetic counselling.

[Experience with medico-genetic counseling of the families of patients with Duchenne muscular dystrophy]. / Opyt mediko-geneticheskogo konsul'tirovaniia semei s bol'nymi myshechnoi distrofiei Diushenna.

The article presents the experience with the use of comprehensive clinico-genealogic and biochemical methods of examination for the purpose of detecting heterozygotic carriership and preventing the birth of children with Duchenne's progressive myodystrophy in the practice of genetic counselling of families in Leningrad.

[Cytoplasmic RNA biosynthesis in Erlich ascitic carcinoma cells under anaerobic conditions]. / Biosintez tsitoplazmaticheskoi RNK v kletkakh astsitnoi kartsinomy Erlikha v anaérobnykh usloviiakh.

Cells of Ehrlich ascites carcinoma were incubated under aerobic and anaerobic conditions with 3H-uridine, and the amount and radioactivity of poly(A)-containing and poly(A)-non-containing fraction of cytoplasmatic RNA were determined. It is shown that RNA biosynthesis, as judged by the labeled precursor incorporation, is nearly similar to that under oxygen supply conditions. The specific radioactivity of poly(A)-containing fraction of cytoplasmatic RNA of cells incubated under anaerobic conditions was 2 times as much as that under aerobic ones.

[RNA biosynthesis in the ascitic cells of Ehrlich's carcinoma and Zajdela's hepatoma under conditions of blocked oxidative phosphorylation]. / Biosintez RNK v astsitnykh kletkakh kartsinomy Erlikha i gepatomy Zaidela v usloviiakh vykliuchennogo okislitel'nogo fosforilirovaniia.

In experiments in vitro on ascites tumor cells of Ehrlich carcinoma and Zajdela hepatoma the author studied the effect of 2,4-dinitrophenol (an agent dissociating respiration from phosphorylation) on respiration, glycolysis, resynthesis of ATP and synthesis of basic fractions of cytoplasmic RNA by the incorporation of labeled 3N-uridine precursor. It was shown that under optimum conditions of tumor cell incubation (phosphate-rich Igle medium) in the presence of 6.10(-4) M DNP a sharp activation of anaerobic glycosis is observed as well as increased O2 absorption and high level of ATP. Blocked phosphorylation associated with respiration renders no appreciable effect on the biosynthesis of basic fractions (4 S, 18 S, 28 S) of cytoplasmic RNA.

[Advantages of the Eagle medium over saline solutions in the studies of the metabolic parameters of tumor cells in vitro]. / Preimushchestva sredy Igla pered solevymi restvorami pri izuchenii in vitro metabolicheskikh parametrov opukholevykh kletok.

In two media (krebsringer phosphate and phosphate-enriched Eagle medium) the indices of energetic metabolism (respiration, glycolysis. ATP content) were determined and the metabolism of macromolecular compounds (c-RNA) of Ehrlich carcinoma ascitic cells was investigated. The results of studies of ascites tumor cells metabolism have indicated considerable advantages of phosphate-enriched Eagle medium over krebs ringer phosphate buffer.