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Identification of ST2A1 as a rat brain neurosteroid sulfotransferase mRNA.
Shimada, M; Yoshinari, K; Tanabe, E; Shimakawa, E; Kobashi, M; Nagata, K; Yamazoe, Y.
Brain Res ; 920(1-2): 222-5, 2001 Nov 30.
Article in English | MEDLINE | ID: mdl-11716829

ABSTRACT

A hydroxysteroid sulfotransferase (ST2A1) was identified as a form mediating neurosteroid sulfation in rat brain. The sole expression among known rat ST2A forms was indicated by brain RT-PCR. All nucleotide sequences of seven ST2A cDNA clones isolated from brain matched completely with that of hepatic ST2A1. The recombinant ST2A1 protein mediated neurosteroid sulfation. These data strongly suggest a functional role of ST2A1 as a neurosteroid sulfotransferase in rat brain.


Subject(s)
Brain/enzymology , RNA, Messenger/biosynthesis , Sulfotransferases/biosynthesis , Animals , Blotting, Western , Corticosterone/pharmacology , Cytosol/enzymology , Dehydroepiandrosterone Sulfate/metabolism , Female , Isoenzymes/genetics , Isoenzymes/metabolism , Liver/enzymology , Male , Phosphoadenosine Phosphosulfate/pharmacology , Pregnenolone/pharmacology , Rats , Reverse Transcriptase Polymerase Chain Reaction , Sulfotransferases/analysis
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