Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
J Oral Biosci ; 66(2): 465-472, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38614428

ABSTRACT

OBJECTIVES: Local anesthetics act on G protein-coupled receptors (GPCRs); thus, their potential as allosteric modulators of GPCRs has attracted attention. Intracellular signaling via GPCRs involves both G-protein- and ß-arrestin-mediated pathways. To determine the effects of local anesthetics on muscarinic acetylcholine receptors (mAChR), a family of GPCRs, we analyzed the effects of local anesthetics on mAChR-mediated Ca2+ responses and formation of receptor-ß-arrestin complexes in the HSY human parotid cell line. METHODS: Ca2+ responses were monitored by fura-2 spectrofluorimetry. Ligand-induced interactions between mAChR and ß-arrestin were examined using a ß-arrestin GPCR assay kit. RESULTS: Lidocaine reduced mAChR-mediated Ca2+ responses but did not change the intracellular Ca2+ concentration in non-stimulated cells. The membrane-impermeant lidocaine analog QX314 and procaine inhibited mAChR-mediated Ca2+ responses, with EC50 values of 48.0 and 20.4 µM, respectively, for 50 µM carbachol-stimulated Ca2+ responses. In the absence of extracellular Ca2+, the pretreatment of cells with QX314 reduced carbachol-induced Ca2+ release, indicating that QX314 reduced Ca2+ release from intracellular stores. Lidocaine and QX314 did not affect store-operated Ca2+ entry as they did not alter the thapsigargin-induced Ca2+ response. QX314 and procaine reduced the carbachol-mediated recruitment of ß-arrestin, and administration of procaine suppressed pilocarpine-induced salivary secretion in mice. CONCLUSION: Local anesthetics, including QX314, act on mAChR to reduce carbachol-induced Ca2+ release from intracellular stores and the recruitment of ß-arrestin. These findings support the notion that local anesthetics and their derivatives are starting points for the development of functional allosteric modulators of mAChR.


Subject(s)
Anesthetics, Local , Calcium , Lidocaine , Parotid Gland , Receptors, Muscarinic , beta-Arrestins , Humans , Anesthetics, Local/pharmacology , beta-Arrestins/metabolism , Calcium/metabolism , Receptors, Muscarinic/metabolism , Receptors, Muscarinic/drug effects , Animals , Mice , Parotid Gland/drug effects , Parotid Gland/metabolism , Lidocaine/pharmacology , Lidocaine/analogs & derivatives , Cell Line , Carbachol/pharmacology , Calcium Signaling/drug effects , Procaine/pharmacology
2.
Methods Mol Biol ; 2582: 295-308, 2023.
Article in English | MEDLINE | ID: mdl-36370358

ABSTRACT

Angiogenesis, the process of generating new blood vessels from an existing vasculature, is essential in normal developmental processes such as endochondral ossification and in numerous kinds of pathogenesis including tumor growth. A part from the actin of angiogenic factor or antiangiogenic factor, it is still unknown at which stage of the angiogenic cascade these agents affect angiogenesis. Here, we describe methods for the use of cellular communication network factor/connective tissue growth factor (CTGF/CCN2) and CCN2-neutralizing antibody in the currently used principal angiogenesis assays, including those in vitro ones for the proliferation, migration, adhesion, and tube formation of endothelial cells and in vivo assays such as those utilizing type I collagen implantation and the chick chorioallantoic membrane (CAM). In addition, we introduce an autofluorescence imaging of blood vessels in the subcutaneous tumor xenograft mouse model. These assays can be applied to studies on roles of CCN proteins in tumor metastasis and development of treatment strategies targeting CCN proteins.


Subject(s)
Endothelial Cells , Neovascularization, Pathologic , Humans , Mice , Animals , Endothelial Cells/metabolism , Neovascularization, Pathologic/pathology , Connective Tissue Growth Factor/metabolism , Chorioallantoic Membrane/blood supply , Angiogenesis Inducing Agents , Biological Assay , Angiogenesis Inhibitors
SELECTION OF CITATIONS
SEARCH DETAIL
...