ABSTRACT
Polymer hydrogels have been suggested as dressing materials for the treatment of cutaneous wounds and tissue revitalization. In this work, we report the development of a hydrogel composed of natural polymers (sodium alginate and gelatin) and silver nanoparticles (AgNPs) with recognized antimicrobial activity for healing cutaneous lesions. For the development of the hydrogel, different ratios of sodium alginate and gelatin have been tested, while different concentrations of AgNO3 precursor (1.0, 2.0, and 4.0 mM) were assayed for the production of AgNPs. The obtained AgNPs exhibited a characteristic peak between 430-450 nm in the ultraviolet-visible (UV-Vis) spectrum suggesting a spheroidal form, which was confirmed by Transmission Electron Microscopy (TEM). Fourier Transform Infra-red (FT-IR) analysis suggested the formation of strong intermolecular interactions as hydrogen bonds and electrostatic attractions between polymers, showing bands at 2920, 2852, 1500, and 1640 cm-1. Significant bactericidal activity was observed for the hydrogel, with a Minimum Inhibitory Concentration (MIC) of 0.50 µg/mL against Pseudomonas aeruginosa and 53.0 µg/mL against Staphylococcus aureus. AgNPs were shown to be non-cytotoxic against fibroblast cells. The in vivo studies in female Wister rats confirmed the capacity of the AgNP-loaded hydrogels to reduce the wound size compared to uncoated injuries promoting histological changes in the healing tissue over the time course of wound healing, as in earlier development and maturation of granulation tissue. The developed hydrogel with AgNPs has healing potential for clinical applications.
ABSTRACT
OBJECTIVE: This study was to evaluate the effects of psychosocial interventions on survival in adult patients with cancer. METHOD: MEDLINE via PubMed, Cochrane Library CENTRAL, CINAHL, and Korean electronic databases (September 2014) were searched. Methodological quality was assessed using Cochrane's Risk of Bias for randomized studies. The RevMan 5.3 program of the Cochrane library was used for data analysis. RESULTS: Fifteen randomized controlled trials met the inclusion criteria, with a total of 2940 participants. Overall, psychosocial interventions was not associated with better survival (HR = .83, 95% CI [.68, 1.10], p = .06, I(2) = 64%). In subgroup analysis, based on six trials with 1448 subjects, psychoeducational interventions for cancer patients with non-metastatic at intervention implementation resulted in a 41% reduction in the risk of dying of cancer (HR = .59, 95% CI [.49, .71], p < .001, I(2) = 0%). For psychoeducational intervention, significant survival benefit were found when health staff delivered the intervention and at a follow-up time of more than 10 years. CONCLUSIONS: Use of psychoeducational interventions for cancer patients at early stage appeared to have beneficial effects on survival, preferably for delivering of health staff. However, conduct of further psychosocial studies with adequate power will lead to better understanding of the effects of treatments on survival outcome.
Subject(s)
Neoplasms/psychology , Neoplasms/therapy , Psychotherapy , Adult , Humans , Randomized Controlled Trials as Topic , Survival Analysis , Treatment OutcomeABSTRACT
The ezrin-radixin-moesin (ERM) proteins are a family of widely distributed membrane-associated proteins and have been implicated not only in cell-shape determination but also in signaling pathway. The nucleus accumbens (NAcc) is an important neuronal substrate mediating the effects of drugs of abuse. However, it has not been determined yet how ERM proteins are regulated in this site by drugs of abuse. Here we show in rat that the phosphorylation levels of ERM protein are dose- and time-dependently decreased in the NAcc by a single injection of cocaine (15 or 30 mg/kg i.p.). Further, we show that the amount of active RhoA, a small GTPase protein, is significantly reduced in the NAcc by cocaine, while the phosphorylation levels of ERM protein are also decreased by bilateral microinjections in this site of the Rho kinase inhibitors. Together, these results suggest that cocaine reduces phosphorylated ERM levels in the NAcc by making downregulation of RhoA-Rho kinase signaling, which may importantly contribute to initiate synaptic changes in this site leading to drug addiction.
Subject(s)
Cocaine/pharmacology , Dopamine Uptake Inhibitors/pharmacology , Nucleus Accumbens/drug effects , Nucleus Accumbens/metabolism , rhoA GTP-Binding Protein/metabolism , Animals , Cocaine/administration & dosage , Cytoskeletal Proteins/metabolism , Dopamine Uptake Inhibitors/administration & dosage , Dose-Response Relationship, Drug , Enzyme Inhibitors/pharmacology , Male , Membrane Proteins/metabolism , Microfilament Proteins/metabolism , Myosin-Light-Chain Phosphatase/metabolism , Phosphorylation/drug effects , Rats , Rats, Sprague-Dawley , Signal Transduction/drug effects , Time Factors , rho-Associated Kinases/antagonists & inhibitorsABSTRACT
Smoking cessation has been identified as an important factor for health and quality of life in Korean society. This article explicates Korean adolescents' experiences of smoking cessation from the perspective of Parse's human becoming theory, as a prelude to conducting research. A nurse was truly present with individual students as they shared their experiences of trying to stop smoking. Interpreted through the principles and concepts of Parse's theory, the experience of quitting smoking is elucidated as a struggle of choosing one's value priorities amid the opportunities and limitations inherent in this decision, while moving with unique patterns of relating. Staying with the commitment to stop smoking is changing one's health and quality of life. The authors briefly discuss the implications of this perspective for nursing practice, and identify struggling to change as a phenomenon for future research.
