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1.
Sci Rep ; 7(1): 11320, 2017 09 12.
Article in English | MEDLINE | ID: mdl-28900183

ABSTRACT

Controversially, histone deacetylase inhibitors (HDACi) are in clinical trial for the treatment of inherited retinal degeneration. Utilizing the zebrafish dye ucd6 model, we determined if treatment with HDACi can rescue cone photoreceptor-mediated visual function. dye exhibit defective visual behaviour and retinal morphology including ciliary marginal zone (CMZ) cell death and decreased photoreceptor outer segment (OS) length, as well as gross morphological defects including hypopigmentation and pericardial oedema. HDACi treatment of dye results in significantly improved optokinetic (OKR) (~43 fold, p < 0.001) and visualmotor (VMR) (~3 fold, p < 0.05) responses. HDACi treatment rescued gross morphological defects and reduced CMZ cell death by 80%. Proteomic analysis of dye eye extracts suggested BDNF-TrkB and Akt signaling as mediators of HDACi rescue in our dataset. Co-treatment with the TrkB antagonist ANA-12 blocked HDACi rescue of visual function and associated Akt phosphorylation. Notably, sole treatment with a BDNF mimetic, 7,8-dihydroxyflavone hydrate, significantly rescued dye visual function (~58 fold increase in OKR, p < 0.001, ~3 fold increase in VMR, p < 0.05). In summary, HDACi and a BDNF mimetic are sufficient to rescue retinal cell death and visual function in a vertebrate model of inherited blindness.


Subject(s)
Blindness/physiopathology , Brain-Derived Neurotrophic Factor/metabolism , Genetic Diseases, Inborn/physiopathology , Molecular Mimicry , Retinal Cone Photoreceptor Cells/metabolism , Vision, Ocular , Animals , Blindness/diagnosis , Blindness/drug therapy , Blindness/genetics , Brain-Derived Neurotrophic Factor/pharmacology , Disease Models, Animal , Electroretinography , Genetic Diseases, Inborn/diagnosis , Genetic Diseases, Inborn/drug therapy , Genetic Diseases, Inborn/genetics , Histone Deacetylase Inhibitors/pharmacology , Male , Mutation , Proteome , Proteomics/methods , Receptor, trkB/metabolism , Retina/drug effects , Retina/metabolism , Retina/pathology , Retinal Cone Photoreceptor Cells/drug effects , Signal Transduction , Vision, Ocular/drug effects , Vision, Ocular/genetics , Zebrafish , Zebrafish Proteins/chemistry , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism
2.
BMC Genomics ; 15: 825, 2014 Sep 30.
Article in English | MEDLINE | ID: mdl-25266257

ABSTRACT

BACKGROUND: The genetic cascades underpinning vertebrate early eye morphogenesis are poorly understood. One gene family essential for eye morphogenesis encodes the retinal homeobox (Rx) transcription factors. Mutations in the human retinal homeobox gene (RAX) can lead to gross morphological phenotypes ranging from microphthalmia to anophthalmia. Zebrafish rx3 null mutants produce a similar striking eyeless phenotype with an associated expanded forebrain. Thus, we used zebrafish rx3-/- mutants as a model to uncover an Rx3-regulated gene network during early eye morphogenesis. RESULTS: Rx3-regulated genes were identified using whole transcriptomic sequencing (RNA-seq) of rx3-/- mutants and morphologically wild-type siblings during optic vesicle morphogenesis. A gene co-expression network was then constructed for the Rx3-regulated genes, identifying gene cross-talk during early eye development. Genes highly connected in the network are hub genes, which tend to exhibit higher expression changes between rx3-/- mutants and normal phenotype siblings. Hub genes down-regulated in rx3-/- mutants encompass homeodomain transcription factors and mediators of retinoid-signaling, both associated with eye development and known human eye disorders. In contrast, genes up-regulated in rx3-/- mutants are centered on Wnt signaling pathways, associated with brain development and disorders. The temporal expression pattern of Rx3-regulated genes was further profiled during early development from maternal stage until visual function is fully mature. Rx3-regulated genes exhibited synchronized expression patterns, and a transition of gene expression during the early segmentation stage when Rx3 was highly expressed. Furthermore, most of these deregulated genes are enriched with multiple RAX-binding motif sequences on the gene promoter. CONCLUSIONS: Here, we assembled a comprehensive model of Rx3-regulated genes during early eye morphogenesis. Rx3 promotes optic vesicle morphogenesis and represses brain development through a highly correlated and modulated network, exhibiting repression of genes mediating Wnt signaling and concomitant enhanced expression of homeodomain transcription factors and retinoid-signaling genes.


Subject(s)
Retina/metabolism , Zebrafish Proteins/genetics , Zebrafish/genetics , Animals , Animals, Genetically Modified/genetics , Binding Sites , Embryonic Development/genetics , Gene Expression Regulation, Developmental , Gene Library , Gene Regulatory Networks , Homeodomain Proteins/chemistry , Homeodomain Proteins/genetics , Homeodomain Proteins/metabolism , Morphogenesis/genetics , Sequence Analysis, RNA , Transcriptome , Wnt Signaling Pathway , Zebrafish Proteins/metabolism
3.
Adv Exp Med Biol ; 801: 97-103, 2014.
Article in English | MEDLINE | ID: mdl-24664686

ABSTRACT

Vacuolar ATPases (v-ATPases) hydrolyze adenosine triphospate (ATP) to pump protons across cell membranes. Mutations in v-ATPase subunits are implicated in three human disorders: distal renal tubular acidosis, osteopetrosis, and cutis laxa type II. In the eye, the role of v-ATPases is only emerging. Mutations in v-ATPase subunits are not linked to human blindness, but altered proton pump function may underlie ocular pathologies. For example, inhibition of v-ATPase by A2E may accentuate age-related macular degeneration (AMD). In animal models, v-ATPase mutations perturb the retinal pigment epithelium (RPE) and photoreceptor outer segment (OS) phagocytosis, an event linked to retinal degeneration. As the RPE plays essential roles in eye development and vision, the study of v-ATPase-induced RPE dysfunction may improve our understanding of RPE diseases.


Subject(s)
Phagocytosis/physiology , Retinal Photoreceptor Cell Outer Segment/enzymology , Retinal Pigment Epithelium/enzymology , Vacuolar Proton-Translocating ATPases/physiology , Vision, Ocular/physiology , Animals , Humans , Vacuolar Proton-Translocating ATPases/genetics , Vacuolar Proton-Translocating ATPases/metabolism
4.
PLoS One ; 7(12): e52177, 2012.
Article in English | MEDLINE | ID: mdl-23300608

ABSTRACT

Our objective was to profile genetic pathways whose differential expression correlates with maturation of visual function in zebrafish. Bioinformatic analysis of transcriptomic data revealed Jak-Stat signalling as the pathway most enriched in the eye, as visual function develops. Real-time PCR, western blotting, immunohistochemistry and in situ hybridization data confirm that multiple Jak-Stat pathway genes are up-regulated in the zebrafish eye between 3-5 days post-fertilisation, times associated with significant maturation of vision. One of the most up-regulated Jak-Stat genes is the proto-oncogene Pim1 kinase, previously associated with haematological malignancies and cancer. Loss of function experiments using Pim1 morpholinos or Pim1 inhibitors result in significant diminishment of visual behaviour and function. In summary, we have identified that enhanced expression of Jak-Stat pathway genes correlates with maturation of visual function and that the Pim1 oncogene is required for normal visual function.


Subject(s)
Gene Expression Regulation, Developmental , Morpholinos/pharmacology , Proto-Oncogene Proteins c-pim-1/antagonists & inhibitors , Vision Disorders/etiology , Zebrafish/metabolism , Animals , Blotting, Western , Electroretinography , Larva/drug effects , Larva/growth & development , Larva/metabolism , Phylogeny , Proto-Oncogene Proteins c-pim-1/genetics , Proto-Oncogene Proteins c-pim-1/metabolism , RNA, Messenger/genetics , Real-Time Polymerase Chain Reaction , Reverse Transcriptase Polymerase Chain Reaction , STAT3 Transcription Factor/genetics , STAT3 Transcription Factor/metabolism , Signal Transduction/drug effects , Suppressor of Cytokine Signaling 1 Protein , Suppressor of Cytokine Signaling 3 Protein , Suppressor of Cytokine Signaling Proteins/genetics , Suppressor of Cytokine Signaling Proteins/metabolism , Vision Disorders/drug therapy , Vision Disorders/pathology , Zebrafish/genetics , Zebrafish/growth & development , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism
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