[An efficiency study of transplantation of allogenic fibroblasts cultivated in collagen gel for the treatment of corneal burn defects in experiment]. / Izuchenie éffektivnosti transplantatsii kul'tivirovannykh allogennykh fibroblastov v kollagenovom gele pri lechenii ozhogovykh defektov rogovitsy v éksperimente.

The paper contains the results of using a mew method applicable to regulation of corneal regeneration, i.e. transplantation of allogenic fibroblasts cultivated in collagen gel within an experimental model of acute alkali corneal burns in rabbits. Two experimental stages, involving 120 animals, were undertaken. The above method was used, at stage 1, on day 2 after trauma and, at stage 2, it was applied in two weeks after ocular burns with profound corneal defects were inflicted. The clinical, morphological and immune-histochemical examinations were used to show a high efficiency of the method in healing the corneal burn wounds; the mechanism of the therapeutic action of the transplant components exerted on regeneration of the affected cornea was investigated; finally, good outlooks for using the discussed method in clinical practice were proven.

[The role of cell proliferation in tubulogenesis of human keratinocytes]. / Rol' proliferatsii v tubulogeneze keratinotsitov cheloveka.

We studied kinetics and role of keratinocyte proliferation in the process of cysto- and tubulogenesis of human postnatal epidermal keratinocytes in collagen gel. The influence of media conditioned by embryonic and postnatal fibroblasts on morphogenesis has been comparatively analyzed. We studied the influence of proliferation inhibitors and inductors on tubulogenesis. The obtained data indicate partial dissociation of migration, proliferation, and differentiation of keratinocytes during morphogenetic processes in culture. We propose a new model considering proliferation of epithelial cells during cysto- and tubulogenesis.

[Proliferation of primary human keratinocytes during histogenesis in culture]. / Proliferatsiia pervichnykh keratinotsitov cheloveka v protsesse tsistogeneza v kul'ture.

We studied formation of epithelial cysts during cultivation of the primary keratinocytes in a collagen gel. Two stages--epithelial spheroid and cysts--can be recognized in the histogenesis process. Keratinocyte migration prevails at the initial stages of morphogenesis. The stage of spheroid can be described by active cell proliferation. Stratification of spheroid epithelium takes place at the stage of epithelial cysts, while the rate of keratinocytes proliferation decreases. Formation of epithelial cysts is induced by morphogene(s) of mesenchymal origin. The obtained data indicate partial dissociation of keratinocyte proliferation and migration during cytogenesis.

[Induction of invasion and epithelial tubulogenesis in primary human keratinocyte culture]. / Induktsiia invazii i épitelial'nogo tubulogeneza v kul'ture pervichnykh keratinotsitov cheloveka.

In this study we used invasion and tubulogenesis assays to measure the morphogenetic potential of primary human keratinocytes in vitro. We also used human embryonic and dermal fibroblasts in coculture with keratinocytes and compared their influence on keratinocyte behavior in these different cell culture systems. The keratinocytes retained morphogenetic potential in vitro. In the presence of embryonic fibroblasts, the keratinocytes formed tubes and invaded collagen gel. A three-dimensional architecture of invasion clusters was reconstructed. We conclude that the cellular mechanisms of invasion and tubulogenesis are similar.

[Morphological and immunohistochemical characteristics of repair processes in nonhealing wounds]. / Morfologicheskaia i immunogistokhimicheskaia kharakteristika reparativnykh protsessov v dlitel'no ne zazhivaiushchikh ranakh.

Sluggish wounds (SW) are characterized by chronic inflammation, abnormal proportion of extracellular matrix (ECM) components, appearance of ECM components that do not occur under normal conditions, this preventing formation of basal membrane (BM) and reepithelization of the wound. 89 biopsies from 25 patients with SW were studied before treatment and in different intervals after transplantation of allogenic cryopreserved epithelium on the wound. Decreased collagen type III content and accumulation of tenastcin in combination with low number of myofibroblasts were observed in ECM before the transplantation. Normalization of quantitative and qualitative composition of ECM components, BM formation, wound reepithelization without scarring were noted after the transplantation; myofibroblast number remained very low. Wound healing without contraction and scarring is a distinctive feature of reparative process in SW when allogenic cryopreserved epidermis is used.