A new chronology for tsunami deposits prior to the 1700 CE Cascadia earthquake from Vancouver Island, Canada.

Coastal deposits at Tofino, Ucluelet, and Port Alberni in Vancouver Island along the Cascadia subduction zone were re-examined to improve the earthquake history of the southwest coast of Canada. We found sand sheets interbedded within peat and mud, suggesting deposition by strong flows in a low-energy environment. Based on limiting maximum and minimum ages derived from plant macrofossils, the age of one of the sand sheets below the tsunami deposits of the great Cascadia earthquake in 1700 CE was estimated to be 1330-1430 CE. Onshore paleoseismic evidence has been documented in Vancouver Island, northern Washington, and northern Oregon during this period. However, the newly constrained age is between those of coseismic subsidence Y and W events in southern Washington, which have been recognized as the 1700 CE and the penultimate Cascadia earthquakes, respectively. Moreover, the new age partly overlaps with the age of offshore paleoseismic evidence for T2, interpreted to have originated from the penultimate Cascadia earthquake, based on offshore turbidite records. The new chronology prior to the 1700 CE Cascadia tsunami deposit from Vancouver Island contributes to a better understand of the timing of the penultimate Cascadia earthquake.

Erosion and sedimentation during the September 2015 flooding of the Kinu River, central Japan.

Erosional and sedimentary features associated with flooding have been documented in both modern and past cases. However, only a few studies have demonstrated the relationship between these features and the corresponding hydraulic conditions that produced them, making it difficult to evaluate the magnitude of paleo-flooding. This study describes the characteristics associated with inundation depth and flow direction, as well as the erosional and sedimentary features resulting from the disastrous flooding of the Kinu River, central Japan, in September 2015. Water levels rose rapidly due to heavy rainfall that eventually overtopped, and subsequently breached, a levee in Joso City, causing destructive flooding on the surrounding floodplain. Distinctive erosional features are found next to the breached levee, while depositional features, such as a sandy crevasse-splay deposit are found further away from the breach. The deposit can be divided into three units based on sedimentary facies. The vertical and lateral changes of these sedimentary facies may be the result of temporal and spatial changes associated with flow during the single flooding event. These observations and quantitative data provide information that can be used to reveal the paleohydrology of flood deposits in the stratigraphic record, leading to improved mitigation of future flooding disasters.

Immunolocalization of water channel aquaporins in human knee articular cartilage with intact and early degenerative regions.

Aquaporins (AQPs), a family of water channel proteins expressed in various cells and tissues, serve as physiological pathways of water and small solute transport. Articular cartilage is avascular tissue with unique biomechanical structure, a major component of which is "water". Our objective is to investigate the immunolocalization and expression pattern changes of AQPs in articular cartilage with normal and early degenerative regions in the human knee joint, which is the joint most commonly involved in osteoarthritis (OA). Two isoforms (AQPs 1 and 3) of AQPs were examined by immunohistochemical analyses using isoform-specific antibodies with cartilage samples from OA patients undergoing total knee arthroplasty. AQP 1 and AQP 3 were expressed in human knee articular cartilage and were localized in chondrocytes, both in the intact and early degenerative cartilage regions. Compared to the intact cartilage, both AQP 1 and AQP 3 immunopositive cells were observed at the damaged surface area in the degenerative region. These findings suggest that these AQPs play roles in metabolic water regulation in articular cartilage of load bearing joints and that they are responsible for OA onset.

Tartrate-Resistant Acid Phosphatase 5b is a Useful Serum Marker for Diagnosis and Recurrence Detection of Giant Cell Tumor of Bone.

Serum tartrate-resistant acid phosphatase (TRACP) 5b was investigated for use as a marker for diagnosis of giant cell tumor (GCT) of bone and for detection of its recurrence.Four patients with GCT of bone who were initially referred to our hospital were classified as a primary group. Three patients who had local recurrence following curettage were classified as a local recurrence group. Five with no recurrence were classified as a no-recurrence group. Eighteen patients with primary and metastatic malignant bone tumors were also enrolled in the study as a control group. Serum TRACP 5b was measured before the biopsy in all patients and was measured periodically after the operation in patients with GCT of bone. Student t-tests were used for statistical analyses.TRACP 5b was greater than 1500 Um/dL in all primary group patients. Mean TRACP 5b values decreased gradually with post-operative time, showing lower values until local recurrence. The mean value of TRACP 5b of the local recurrence group (753 ± 68.7 mU/dL) was significantly higher than that of the no-recurrence group (340.6 ± 78.3 mU/dL). The mean value of TRACP 5b of the control group (466.9 ± 130.3 mU/dL) was much lower than that of the primary group and markedly lower than that of the local recurrence group. However, no significant difference was found between the no-recurrence group and the control group.Serum TRACP 5b is a useful and convenient marker for diagnosing GCT of bone and for predicting its recurrence.

Solid variant of aneurysmal bone cyst in the tibia treated with simple curettage without bone graft: a case report.

The solid variant of aneurysmal bone cyst (solid ABC) is rarely encountered in long bones and appropriate treatment for this disease remains unclear. We experienced a 13-year-old boy suffering from pain in his left knee caused by solid ABC. Simple curettage of the bone lesion without any adjuvant therapy and a bone graft gave immediate pain relief. Histological examination of the surgical specimen showed typical features of solid ABC, and cycloxygenase-2 (COX-2) expression was confirmed in giant cells with a background of spindle cells by immunohistochemistry. Magnetic resonance imaging showed that soft tissue edema surrounding the lesion was improved two months after surgery and there was no indication of recurrence two years after surgery. If COX-2 secreted from the tumor induces soft tissue edema, simple curettage of the bone lesion seems to be a reasonable treatment for solid ABC and is able to minimize invasive treatment of the patients.

Vascular endothelial growth factor-D is a key molecule that enhances lymphatic metastasis of soft tissue sarcomas.

Studies on lymph node metastasis of soft tissue sarcomas are insufficient because of its rarity. In this study, we examined the expressions of vascular endothelial growth factor (VEGF)-C and VEGF-D in soft tissue sarcomas metastasized to lymph nodes. In addition, the effects of the two molecules on the barrier function of a lymphatic endothelial cell monolayer against sarcoma cells were analyzed. We examined 7 patients who had soft tissue sarcomas with lymph node metastases and who had undergone neither chemotherapy nor radiotherapy before lymphadenectomy. Immunohistochemistry revealed that 2 of 7 sarcomas that metastasized to lymph nodes expressed VEGF-C both in primary and metastatic lesions. On the other hand, VEGF-D expression was detected in 4 of 7 primary and 7 of 7 metastatic lesions, respectively. Interestingly, 3 cases that showed no VEGF-D expression at primary sites expressed VEGF-D in metastatic lesions. Recombinant VEGF-C at 10(-8) and VEGF-D at 10(-7)and 10(-8)g/ml significantly increased the random motility of lymphatic endothelial cells compared with controls. VEGF-D significantly increased the migration of sarcoma cells through lymphatic endothelial monolayers. The fact that VEGF-D induced the migration of fibrosarcomas through the lymphatic endothelial monolayer is the probable reason for the strong relationship between VEGF-D expression and lymph node metastasis in soft tissue sarcomas. The important propensities of this molecule for the increase of lymph node metastases are not only lymphangiogenesis but also down-regulation of the barrier function of lymphatic endothelial monolayers, which facilitates sarcoma cells entering the lymphatic circulation.

Characteristics of neck and shoulder pain (called katakori in Japanese) among members of the nursing staff.

BACKGROUND: The characteristics of neck and shoulder pain (NSP), called katakori in Japanese, have not been well documented to date. The aim of this study was to clarify the characteristics of NSP through a questionnaire survey of members of the nursing staff. METHODS: The study population consisted of 484 nursing staff members of Gunma University Hospital in Japan. The questionnaire involved information on age, body mass index (BMI), gender, psychological stress at work, musculoskeletal pain at other anatomic sites (elbow/wrist, lumbar and knee), smoking history, and hypertension. If subjects had NSP, they were asked about any coexisting symptoms, the utilization of health services, and the precise location of NSP. RESULTS: The total study population included 393 persons after 91 persons were excluded for various reasons. The point prevalence of NSP was 68.1% (268 of 393). Age, BMI, smoking history, and hypertension showed no significant trend for the prevalence of NSP in the univariate analyses. The occurrence of NSP was significantly higher in subjects with psychological stress, elbow/wrist pain, lumbar pain, and knee pain, respectively. A multivariate logistic regression analysis showed that gender, psychological stress, elbow/wrist pain, and lumbar pain were significantly associated with the occurrence of NSP. One hundred fifty-eight of those with NSP (58.9%) reported coexisting symptoms, and the most common was headache. Fifty-seven (21.2%) of the subjects with NSP had consulted medical or health practitioners, and bone setting was the most common service provider. The most common area of NSP was the superior part of the trapezius. CONCLUSIONS: This study confirmed that NSP, katakori in Japanese, is a prevalent problem in a nursing staff, and several factors associated with NSP were identified.

CT fluoroscopy-guided radiofrequency ablation of osteoid osteoma with double nidi.

Osteoid osteoma (OO) is a common benign bone tumor, usually found in children and young adults. Fewer than 30 cases of multicentric OO in a single bone have been reported in the world literature. We present the case of a 17-year-old boy with OO in the left tibia, containing double nidi. Plain radiography and computed tomography (CT) revealed two adjacent nidi surrounded by bony sclerosis within the distal left tibia. The tumor was successfully treated using two separate CT fluoroscopy-guided percutaneous radiofrequency ablations during a single session. The patient achieved complete pain relief the next day, and has remained asymptomatic for 9 months since treatment.

Percutaneous radiofrequency ablation of osteoid osteoma using cool-tip electrodes without the cooling system.

PURPOSE: The aim of this study was to evaluate the efficacy of percutaneous radiofrequency ablation (RFA) for osteoid osteoma (OO) using cool-tip electrodes without the cooling system. MATERIALS AND METHODS: A total of 17 patients (13 males, 4 females; mean age 19.1 years; range 7-49 years) with OO (tibia, n = 7; femur, n = 5; acetabulum, n = 2; radius, n = 1; talus, n = 1; lumbar spine, n = 1) underwent RFA. Using a cool-tip electrode without the cooling system, the lesion was heated to 90°C for 4 or 5 min. Procedures were considered technically successful if the electrode was placed into the nidus and the target temperature was reached and maintained for at least 4 min. Clinical success of the treatment was defined as complete or partial pain relief after RFA. RESULTS: All procedures were considered technically successful, although two patients encountered complications (pes equinus contracture, skin burn). Altogether, 16 of the 17 patients (94.1%) achieved complete or partial pain relief after primary RFA. Two patients had pain recurrence, with one of them treated successfully with a second RFA. The overall clinical success rate was 88.2%. Histological findings confirmed the presence of OO in 13 patients (76.5%). CONCLUSION: Percutaneous RFA of OO using cool-tip electrodes without the cooling system is a safe, effective procedure.

Solitary osteochondroma of the trapezium: case report.

Osteochondroma of the carpal is rare. We found only 1 case of osteochondroma of the trapezium in the literature. We present a case of a 52-year-old woman with an osteochondroma of the left trapezium and trapeziometacarpal arthritis.

Clinical and biochemical characteristics after intra-articular injection for the treatment of osteoarthritis of the knee: prospective randomized study of sodium hyaluronate and corticosteroid.

BACKGROUND: Intra-articular injections of sodium hyaluronate (Na-HA) and corticosteroid (CS) are often used for the treatment of osteoarthritis (OA), and the clinical usefulness of such treatment has been reported. Some studies have discussed the effectiveness of intra-articular drug injection therapy in terms of the clinical results, but no cohort studies have performed evaluations of effectiveness based on changes in joint biomarkers. This prospective randomized study compared the efficacy of Na-HA and CS injections based on clinical scores and levels of biochemical markers for osteoarthritis. METHODS: A total of 51 patients with knee osteoarthritis received intra-articular injections of either Na-HA or CS and were followed for 6 months after treatment. Pain and inflammatory scores were evaluated at the baseline, at 5 weeks, and at 6 months. We also measured joint fluid levels of hyaluronan (HA), chondroitin 6-sulfate, chondroitin 4-sulfate, matrix metalloproteinase (MMP)-9, and tissue inhibitor of MMP (TIMP)-1 at the baseline and at 5 weeks. RESULTS: In both groups, injection therapy significantly improved pain/inflammation scores and visual analog scale scores with time (P < 0.01). HA levels were significantly increased after injection only in the Na-HA group (P < 0.05); and the MMP-9 level decreased significantly after injection only in the Na-HA group (P < 0.01). Other marker levels did not differ significantly between groups. CONCLUSIONS: The results of this prospective randomized study suggest that the clinical effects of Na-HA and CS as local therapies for OA are comparable and that both drugs are useful. Considering the results of the measurement of biomarkers, compared with CS injection therapy Na-HA injection therapy may have protective effects on the articular cartilage by increasing the HA concentration in synovial fluid, as well as inhibitory effects on the catabolism of articular cartilage by reducing the MMP-9 concentration.

Fibroma of tendon sheath of the infrapatellar fat pad in the knee.

A 69-year-old patient with unexplained pain of the right knee reported no prior trauma. A soft tissue tumor was identified using magnetic resonance imaging (MRI). The tumor showed low signal intensity on T1-weighted images, high signal intensity on T2, and peripheral enhancement on gadolinium-enhanced MRI. Perioperative observation revealed that the tumor was partly adhesive to the anterior knee joint capsule, but not to the patellar tendon.

Konjac glucomannan-based hydrogel with hyaluronic acid as a candidate for a novel scaffold for chondrocyte culture.

Chondrocytes were cultured using konjac glucomannan (KGM) and hyaluronic acid (HA) as a scaffold for cartilage regeneration. They were subsequently compared with scaffolds produced using agarose hydrogels. Chondrocytes derived from Japanese white rabbits were cultured: 2.0 x 10(5) cells were seeded on KGM containing hyaluronic acid (KGM/HA) and agarose and cultured for 5 days. Their viability was assayed using WST-8 procedures; the ultimate stress and modulus of elasticity of each construct was calculated. After 3 days of cultivation, mRNA in chondrocytes, such as collagen types I and II and aggrecan, were measured using RT-PCR. Both chondrocyte-seeded constructs were stained with safranin O/fast green and were evaluated histologically. Chondrocyte viability decreased concomitantly with increasing KGM/HA or agarose concentration and with culture time. Cell viability in 2% agarose was significantly lower than that in 2% KGM/HA on the third and fifth days (p < 0.05). The primary elastic modulus increased concomitantly with increasing polysaccharide concentration. Elastic moduli of 2% KGM/HA with chondrocytes (0.389 +/- 0.119 N/mm(2)) showed little difference from those without chondrocytes (0.283 +/- 0.243 N/mm(2)), although those of 2% agarose with chondrocytes (0.403 +/- 0.094 N/mm(2)) were significantly lower than those without chondrocytes (0.736 +/- 0.227 N/mm(2); p < 0.05). Collagen type II mRNA expression was higher in KGM/HA and agarose than in monolayer cultures, although KGM/HA had lower aggrecan mRNA expression levels than did agarose. Histological tests of KGM/HA-chondrocyte constructs revealed chondrocyte aggregation and proteoglycan production in the pericellular region. The results show that KGM/HA might be useful for chondrocyte culture.

Involvement of the proximal tibial epiphysis by monostotic fibrous dysplasia.

We report a case of monostotic fibrous dysplasia (FD) solely demonstrating epiphyseal lesion. A 13-year-old boy was referred to our hospital complaining of pain in his left knee joint. Plain radiographs showed an osteolytic lesion with slightly sclerotic margin at the proximal epiphysis of the left tibia. The lesion was shown with low intensity on T(1)-weighted and high on T(2)*-weighted magnetic resonance imaging. Contrast-enhanced T(1)-weighted images showed marginal enhancement of the lesion. Histological examination of the surgical specimens showed FD.

Curettage of benign bone tumors without grafts gives sufficient bone strength.

BACKGROUND AND PURPOSE: The defect that results after curettage of a bone tumor is usually filled in the same way. We report the outcome in patients with benign bone tumors that were treated with curettage but no filling. PATIENTS AND METHODS: We retrospectively studied 78 patients (mean age at the time of operation was 27 (6-73) years, 44 men) who had had a benign bone tumor curetted with no filling of the defect. The commonest tumor types were giant cell tumor of bone (27), fibrous dysplasia (13), enchondroma (9), and simple bone cyst (7). The mean size of the lesions was 35 (2-196) cm(3). Normal activities, including full weight bearing for lower extremity lesions, were allowed after 3 months or less. The patients were followed for an average of 10 (1.2-21) years. RESULTS: A postoperative fracture with a minor displacement occurred in 3 patients, in 2 of them because of local recurrence. All fractures healed. Local recurrence occurred in 9 patients; 7 of them had a giant cell tumor. Repeated local recurrences necessitated above-knee amputation in 1 patient. All other patients had unrestricted activities of daily living. INTERPRETATION: Routine filling of curetted bone lesions does not appear to be necessary from a mechanical point of view.

Stimulatory role of lysophosphatidic acid in cyclooxygenase-2 induction by synovial fluid of patients with rheumatoid arthritis in fibroblast-like synovial cells.

While inflammatory cytokines are well-recognized critical factors for the induction of cyclooxygenase-2 (COX-2) in activated fibroblast-like synovial cells, the roles of biologically active components other than inflammatory cytokines in synovial fluid remain unknown. Herein, we assessed the role of lysophosphatidic acid (LPA), a pleiotropic lipid mediator, in COX-2 induction using synovial fluid of patients with rheumatoid arthritis (RA) in fibroblast-like RA synovial cells. Synovial fluid from RA patients stimulated COX-2 induction, which was associated with prostaglandin E(2) production, in RA synovial cells. The synovial fluid-induced actions were inhibited by G(i/o) protein inhibitor pertussis toxin and LPA receptor antagonist 3-(4-[4-([1-(2-chlorophenyl)ethoxy]carbonyl amino)-3-methyl-5-isoxazolyl] benzylsulfanyl) propanoic acid (Ki16425). In fact, LPA alone significantly induced COX-2 expression and enhanced IL-1alpha- or IL-1beta-induced enzyme expression in a manner sensitive to pertussis toxin and Ki16425. RA synovial cells abundantly expressed LPA(1) receptor compared with other LPA receptor subtypes. Moreover, synovial fluid contains a significant amount of LPA, an LPA-synthesizing enzyme autotaxin, and its substrate lysophosphatidylcholine. In conclusion, LPA existing in synovial fluid plays a critical role in COX-2 induction in collaboration with inflammatory cytokines in RA synovial cells. Ki16425-sensitive LPA receptors may be therapeutic targets for RA.

Pre- and postoperative evaluation of the metabolic activity in muscles associated with ruptured rotator cuffs by F-FDG PET imaging.

We examined uptake of 2-[(18)F]-fluoro-2-deoxy-D-glucose (FDG) using positron emission tomography (PET) into the bilateral shoulder muscles of four patients before and after unilateral rotator cuff repair. The relative ratio of the subtracted standardized uptake value on the supraspinatus with a torn supraspinatus tendon to that of the subscapularis on the ipsilateral side was significantly lower than that on the normal side (0.69 +/- 0.05 versus 1.06 +/- 0.36). The relative ratio of the infraspinatus on the rupture side was also significantly lower than that on the normal side (0.91 +/- 0.09 versus 1.41 +/- 0.35). However, by 6 months postoperative, the reduced glucose metabolism in these muscles had recovered to the same levels as those on the normal side. As indicated by FDG PET, muscle metabolism in rotator cuff tears was reduced, subsequently recovering to the level of the normal side after operative repair.