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1.
J Radiol Prot ; 34(3): 675-98, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25125455

ABSTRACT

Data related to radioactivity released from the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident on 15 March 2011 gathered by residents of Miharu, Fukushima Prefecture, and by Tohoku University are presented. These data sets consist of (1) the earliest radiation monitoring by a Geiger counter in the town, (2) ratios of radioactivity between (132)Te and (137)Cs for a wide area between Fukushima and Tokyo, (3) radiation measurement of soil samples collected from 18 school grounds, and (4) external radiation exposure of 1400 students using OSL badges. By combining and analysing these various data sets, a curve for the cumulative total external exposure as a function of time, with 16 : 00 h on 15 March 2011 being time zero, is obtained. The average cumulative external dosage is estimated to be 10 mSv (σ = 4.2 mSv) over 10 years. In addition, the initiative that the residents of Miharu took in response to the FDNPP accident, which became known as The Misho Project (MP), is documented; in particular, the time at which the municipality instructed the immediate ingestion of iodine tablets by those under the age of 40, 13 : 00 h on 15 March 2011, is assessed.


Subject(s)
Fukushima Nuclear Accident , Nuclear Power Plants , Radiation, Ionizing , Radiometry/statistics & numerical data , Japan
2.
Appl Radiat Isot ; 65(1): 32-5, 2007 Jan.
Article in English | MEDLINE | ID: mdl-16908177

ABSTRACT

A technique for preparing nuclear reaction targets of various thicknesses was developed by using common filtration technique of hydroxide precipitates with a porous Al(2)O(3) membrane filter. Uniformity was found to be within a few % in each thickness. Durability for beam irradiation was also confirmed. The preparation procedure is convenient and the method is appropriate for several target materials, including not only precious materials but also radioactive materials with low contamination.


Subject(s)
Aluminum Oxide/chemistry , Aluminum Oxide/radiation effects , Fractional Precipitation , Heavy Ions , Nuclear Physics/methods , Ultrafiltration/methods , Materials Testing
3.
Int J Gynecol Cancer ; 15(1): 155-62, 2005.
Article in English | MEDLINE | ID: mdl-15670311

ABSTRACT

Loss of heterozygosity (LOH) was analyzed in four patients with endometrial hyperplasia (EH) with atypia (two patients) and without atypia (two patients) and in five patients with endometrial adenocarcinoma (EAC) to clarify the clinicopathologic relationship between genetic alterations and hormone therapy. Each patient was initially administered high-dose medroxyprogesterone acetate (MPA) as a uterine-sparing treatment. The five microsatellite markers used to analyze LOH were at chromosomal loci 8p22.1, 8p21, 8p21.3, 8p22, and 8p22. DNA was extracted from paraffin-embedded sections before, during, and after MPA therapy using laser capture microdissection. As a result, LOH was more frequently detected after MPA therapy (overall ratios were 16, 17, and 29% before, during, and after MPA therapy, respectively). LOH is more easily detected in EH loci than in EAC loci before MPA. For EAC, initial LOH detection on chromosome 8 may be related to an incomplete response to MPA, but negative LOH does not guarantee a favorable treatment outcome. For EH or atypical endometrial hyperplasia, it is unknown whether LOH alteration associated with MPA therapy is related to atypia of the disease.


Subject(s)
Adenocarcinoma/genetics , Endometrial Hyperplasia/genetics , Endometrial Neoplasms/genetics , Loss of Heterozygosity/drug effects , Adenocarcinoma/drug therapy , Adult , Antineoplastic Agents, Hormonal/therapeutic use , Endometrial Hyperplasia/drug therapy , Endometrial Neoplasms/drug therapy , Female , Humans , Medroxyprogesterone/therapeutic use
4.
Bone Marrow Transplant ; 33(6): 589-95, 2004 Mar.
Article in English | MEDLINE | ID: mdl-14743198

ABSTRACT

Repeated high-dose chemotherapy (HDC) with stem cell support is advocated for curative treatment of epithelial ovarian cancer patients, requiring large quantities of progenitor cell harvest. Although the switchover to peripheral blood stem cell transplantation has generally made possible the harvest of large quantities of progenitor cells, the minimum threshold is still pertinent for planning the safe conduct of HDC. However, as the minimum threshold for safe peripheral blood stem cell transplantation (PBSCT) is not yet established, this study was designed to clarify the minimum amount of progenitor cells required for prompt recovery of hematopoietic. Retrospective analysis was performed on 52 HDCs administered in 37 ovarian cancer patients. After autologous bone marrow aspiration (10 patients) or peripheral blood stem cell harvest (27 patients), colony-forming unit granulocyte macrophage (CFU-GM) were enumerated prior to cryopreservation. Numbers of CFU-GM were again calculated before reinfusion and the patients were divided into eight groups: 0.13-<0.4, 0.4-<0.7, 0.7-<1.0, 1.0-<3.5, 3.5-<5.0, 5.0-<10.0, 10.0-<20.0 and >20.0 (x 10(5)/kg). The minimum CFU-GM threshold (x 10(5)/kg) was found to be 1.0-<3.5 for platelets and 3.5-<5.0 for white blood cells. Higher infusion doses did not lead to significant benefits in hematopoietic reconstruction. These results indicate that preservation of a minimum of 7-10 x 10(5)/kg CFU-GM is recommended for the safe conduct of tandem HDCs.


Subject(s)
Ovarian Neoplasms/therapy , Stem Cell Transplantation/methods , Female , Hematopoietic Stem Cells/cytology , Humans , Leukocyte Count , Platelet Count , Platelet Transfusion , Retrospective Studies , Transplantation, Autologous
5.
J Forensic Sci ; 47(6): 1391-6, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12455670

ABSTRACT

A rare case of colchicine poisoning resulting from accidental ingestion of meadow saffron (Colchicun Autumnale) is reported. The plant can frequently be found in the woods of the Northern Hemisphere (1), also in Japan. A 48-year-old male was admitted to hospital complaining of vomiting, nausea and diarrhea following ingestion of the plant and died in four days. The most striking histological findings were metaphasic mitotic figures in the mucosa of the large intestine and the liver. Colchicine was detected in the bile with high-performance liquid chromatography/sonicspray ionization mass spectrometry (HPLC/SSI-MS).


Subject(s)
Colchicine/poisoning , Colchicum/poisoning , Accidents , Chromatography, High Pressure Liquid , Colon/pathology , Diarrhea/chemically induced , Fatal Outcome , Forensic Medicine , Humans , Intestinal Mucosa/pathology , Liver/pathology , Male , Middle Aged , Nausea/chemically induced , Spectrometry, Mass, Electrospray Ionization , Vomiting/chemically induced
6.
Int J Legal Med ; 115(2): 82-4, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11724436

ABSTRACT

We previously reported the polymorphism of the high density lipoprotein-associated enzyme paraoxonase (PON1), in the 10 sarin poisoning victims in the Tokyo subway terrorist attack. Arg192 PON1, which has low sarin hydrolysing activity, was found to be more common in the Japanese population than in people of other races. However, from our analyses seven of the victims expressed the PON1 phenotype with high sarin hydrolysing activity and three with low sarin hydrolysing activity. These results indicate that the main factor contributing to the tragedy of the Tokyo subway terrorist attack was the high toxicity of sarin rather than the race-dependent genetic difference in the Arg192 PON1 polymorphism.


Subject(s)
Chemical Warfare Agents/poisoning , Esterases/genetics , Isoenzymes/genetics , Sarin/poisoning , Terrorism , Aged , Aged, 80 and over , Alleles , Aryldialkylphosphatase , Base Sequence , DNA Primers , Female , Gene Frequency , Humans , Male , Middle Aged , Poisoning/genetics , Tokyo
7.
Tokai J Exp Clin Med ; 24(1): 21-7, 1999 Apr.
Article in English | MEDLINE | ID: mdl-10530622

ABSTRACT

PURPOSE: To assess the effects of vaginal applicators (ovoids) on dose distribution, local control and rectal complications in cervical cancers treated by brachytherapy. METHODS: From 1984 to 1992, 41 patients (15 stages I+II; 26 stages III+IV) were treated by high-dose-rate brachytherapy (20 Gy in 4 fractions) after external beam irradiation (36-56 Gy). Twenty-three patients were treated by standard application of both intrauterine tandem and ovoids, and 18 patients were treated by non-standard application. RESULTS: The five-year, local-relapse-free rates by standard application and tandem alone were 69 and 65% overall; 83 and 100% for stages I+II; and 62 and 49% for stages III+IV. Local control was related to tumor response following the external beam irradiation and initial tumor size by multivariate analysis. Rectal complications seen in patients followed for more than 1 year were 33% after standard application and 22% for tandem use alone. The rectal doses given for standard application (24 Gy) and tandem use (16 Gy) were significantly different. CONCLUSIONS: The ovoid sources did not always contribute to local control, and occasionally led to rectal complications. The optimization of brachytherapy was dependent on patients' anatomy, tumor size, and tumor response.


Subject(s)
Brachytherapy/adverse effects , Rectal Diseases/prevention & control , Uterine Cervical Neoplasms/radiotherapy , Vagina , Adult , Aged , Aged, 80 and over , Brachytherapy/methods , Female , Follow-Up Studies , Humans , Middle Aged , Prognosis , Radiation Dosage , Rectal Diseases/etiology , Survivors , Uterine Cervical Neoplasms/complications
8.
Clin Chim Acta ; 285(1-2): 143-54, 1999 Jul.
Article in English | MEDLINE | ID: mdl-10481930

ABSTRACT

We herein report a case of a 5-month-old Japanese female (patient AN) with fasting hyperchylomicronemia due to a primary lipoprotein lipase (LPL) deficiency. Patient AN was compound heterozygous for a missense mutation (GG818G-->GAG/Gly188-->Glu; G188E) in exon 5 and a nonsense mutation (TGG1401-->TGA/Trp382-->Stop; W382X) in exon 8 of the LPL gene. This resulted in less than 10% of the control levels for both the LPL activity and immunoreactive LPL mass in the postheparin plasma. A G188E mutation was thus identified for the first time in a Japanese, and the haplotype of this G188E allele was different from that of the G188E alleles identified in other ethnic groups. This additional mutation might be useful for early diagnosis of LPL gene aberrations in Japanese patients with fasting hyperchylomicronemia.


Subject(s)
Chylomicrons/blood , Heterozygote , Hyperlipidemias/genetics , Mutation , Base Sequence , DNA Primers , Female , Haplotypes , Humans , Hyperlipidemias/enzymology , Infant , Japan , Lipoprotein Lipase/metabolism , Male , Pedigree
9.
J Biomater Sci Polym Ed ; 10(3): 271-82, 1999.
Article in English | MEDLINE | ID: mdl-10189096

ABSTRACT

To improve the surface blood compatibility on a cellulose hemodialysis membrane, 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers with a phospholipid polar group were immobilized on the surface through covalent bonding. The MPC polymers had a carboxylic group, which can react with hydroxyl groups on the cellulose membrane, and were synthesized by conventional radical polymerization. The reaction between the MPC polymers and the cellulose membrane was carried out in a heterogeneous system using a condensation reagent. Surface analysis of the modified membrane by X-ray photoelectron spectroscopy revealed the immobilization of the MPC polymer on the surface. The mechanical strength and permeability for a solute of the membrane did not change even after the modification. The modified cellulose membrane was blood-compatible, as determined by the prevention of adhesion, deformation, and aggregation of platelets after contact with platelet-rich plasma. Based on these results, it is concluded that the MPC polymers may be a useful material for improving the blood compatibility of cellulose hemodialysis membranes.


Subject(s)
Biocompatible Materials , Membranes, Artificial , Methacrylates/chemistry , Phosphorylcholine/analogs & derivatives , Renal Dialysis/instrumentation , Adsorption , Animals , Blood Proteins/chemistry , Cellulose/chemistry , Complement System Proteins/chemistry , Complement System Proteins/metabolism , Complement System Proteins/physiology , Humans , Materials Testing , Permeability , Phosphorylcholine/chemistry , Platelet Adhesiveness , Polymethacrylic Acids , Rabbits , Surface Properties
10.
Cancer ; 85(7): 1555-64, 1999 Apr 01.
Article in English | MEDLINE | ID: mdl-10193946

ABSTRACT

BACKGROUND: In this study, the authors reviewed long term results and prognostic factors of high dose chemotherapy (HDC) with autologous stem cell support administered to 105 patients with epithelial ovarian carcinoma. METHODS: Prior to HDC, platinum-based chemotherapy was given to optimize (n = 94) and/or to mobilize peripheral blood stem cells (n = 33). After maximum debulking surgery, HDC with stem cell support was given; it consisted of cyclophosphamide, doxoribicin, and cisplatin (Regimen A, administered to 58 patients) or cyclophosphamide and carboplatin (Regimen B, administered to 47 patients). RESULTS: Five-year overall and disease free survival (OS, DFS) rates (%) according to stage were IC: 92.3, 92.3; II: 73.3, 73.3; III: 58.1, 35.7; IV: 33.7, 22.6; relapsed: 37.5, 31.0; OS, DFS at 8 years were IC: 92.3, 92.3; II: 73.3, 73.3; III: 48.8, 31.7; IV: 33.7, 22.6; relapsed: 37.5, 31.0. There was no difference in survival between patients who received Regimens A and B despite an increase in the total dose of platinum and an increase of more than 1.5- to 3.5-fold in the platinum course of HDC in Regimen B from the equivalence ratio of 4:1 between carboplatin and cisplatin. Among 65 Stage III and IV patients, the best results were obtained for 35 patients with small volume disease: 5-year OS, DFS rates were 74.3, 51.6, and 8-year OS, DFS rates were 66.4, 46.3. CONCLUSIONS: Good long term results were obtained with HDC. Small volume residual disease, platinum sensitivity, and histology excluding mucinous and clear cell adenocarcinoma were important factors for better survival. However, because the results were obtained for selected patients, a prospective, randomized study comparing HDC and standard chemotherapy is necessary if any definitive conclusions are to be drawn.


Subject(s)
Hematopoietic Stem Cell Transplantation , Ovarian Neoplasms/therapy , Adenocarcinoma, Clear Cell/pathology , Adenocarcinoma, Clear Cell/therapy , Adenocarcinoma, Mucinous/pathology , Adenocarcinoma, Mucinous/therapy , Adult , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Carboplatin/administration & dosage , Cisplatin/administration & dosage , Combined Modality Therapy , Cyclophosphamide/administration & dosage , Doxorubicin/administration & dosage , Female , Humans , Middle Aged , Ovarian Neoplasms/mortality , Survival Rate , Transplantation, Autologous , Treatment Outcome
11.
Int J Gynecol Pathol ; 17(1): 75-8, 1998 Jan.
Article in English | MEDLINE | ID: mdl-9475196

ABSTRACT

An epithelial ovarian tumor with estrogenic manifestations (vaginal bleeding, vaginal softening) was encountered in a 70-year-old woman. The preoperative serum levels of estrone, estradiol, and testosterone were elevated. A right ovarian serous papillary tumor of borderline malignancy was removed and found to have a cellular, focally luteinized stroma. Steroidogenic enzymes, including 3beta-hydroxysteroid dehydrogenase and P450 cytochromes specific for cholesterol side-chain cleavage (P450scc), 17alpha-hydroxylation (P45017alpha), and aromatization (P450arom), were immunohistochemically demonstrated in the stromal cells. Immunohistochemical evaluation of these enzymes suggest significant functional differences among the enzymatically-activated stromal cells. That only rare stromal cells were immunoreactive for P450arom suggests that most of the testosterone synthesized in the tumor is extragonadally converted to estrogen, resulting in tumor-associated estrinism.


Subject(s)
Ovarian Neoplasms/blood , Ovarian Neoplasms/pathology , 3-Hydroxysteroid Dehydrogenases/metabolism , Aged , Biomarkers, Tumor/blood , Cytochrome P-450 Enzyme System/metabolism , Epithelium/enzymology , Epithelium/pathology , Estradiol/blood , Estrone/blood , Female , Humans , Immunohistochemistry , Ovarian Neoplasms/enzymology , Postmenopause , Stromal Cells/pathology , Testosterone/blood
12.
Biol Pharm Bull ; 20(11): 1141-6, 1997 Nov.
Article in English | MEDLINE | ID: mdl-9401720

ABSTRACT

Three sialoglycoprotein fractions having reactivities against influenza A and B viruses (IV-A and IV-B) and influenza virus-hemagglutinin (IV-HA), were obtained by gel filtration using a Sephacryl S-300 column and an Asahipak GS-710 HPLC column from water-soluble fraction prepared from human meconium (ME-WSF). Molecular weights of sialoglycoproteins in these fractions were estimated as 746 kDa (P-Fr. 1-3), 4470 kDa (P-Fr. 1-2) and a more than 10,000 kDa (P-Fr. 1-1), respectively. The other in P-Fr. 2, with molecular weight estimated as 92 kDa, revealed positive but weak reactivity against IV-HA; however, the fraction did not show positive reactivity against either IVs-A or -B. All these fractions contained sialoglycoproteins with a high content of carbohydrates (31.0-59.3%, w/w). From their carbohydrate compositions, it can be suspected that sialoglycoproteins having O-glycosidically linked carbohydrate chains were predominating in the three fractions. In conclusion, it is suggested that at least three sialoglycoproteins with influenza virus-receptor activity showing different chemical properties to each other exist in human meconium.


Subject(s)
Meconium/chemistry , Orthomyxoviridae/metabolism , Receptors, Virus/metabolism , Sialoglycoproteins/isolation & purification , Amino Acids/analysis , Carbohydrates/analysis , Chromatography, Gel , Chromatography, High Pressure Liquid , Humans , Sialoglycoproteins/chemistry , Sialoglycoproteins/metabolism
13.
Acta Cytol ; 41(5): 1451-5, 1997.
Article in English | MEDLINE | ID: mdl-9305383

ABSTRACT

OBJECTIVE: To ascertain the usefulness of endometrial cytology with ovarian cancers when examining extension of the disease and to analyze significant factors associated with migration of ovarian cancer cells into the uterine cavity. STUDY DESIGN: Cytologic results on ascites and the endometrium were analyzed in 87 patients with primary ovarian cancer in the absence of metastasis to the endometrium or cervicovagina. RESULTS: Positive results for cytology were found in 62/87 of ascites cases (71.3%) and in 20/87 endometrium cases (23.0%). The 15 cases (15/62 or 24.2%) positive for ascitic and endometrial cytology, divided clinically into stage III (6 cases) and stage IV (9 cases), were classified histologically as serous, 7 cases; mucinous, 2 cases; clear cell, 4 cases; endometrioid, 1 case; and unclassified, 1 case. Half the clear cell carcinomas (4/8 or 50.0%) were positive in the ascites and endometrium. The ascitic volume at surgery exceeded 500 mL in 9/15 cases (60.0%). CONCLUSION: Papillae with basement membrane material in the cores may be structurally associated with migration of ovarian cancer cells into the uterine cavity, especially in clear cell carcinomas. Cytologic positivity of the endometrium and ascites significantly correlated with ascitic volume.


Subject(s)
Ascites/pathology , Carcinoma/pathology , Endometrium/pathology , Ovarian Neoplasms/pathology , Adenocarcinoma, Clear Cell/pathology , Adenocarcinoma, Mucinous/pathology , Adult , Aged , Biopsy, Needle , Carcinoma, Endometrioid/pathology , Cystadenocarcinoma, Serous/pathology , Evaluation Studies as Topic , Female , Humans , Middle Aged , Vaginal Smears
14.
Pathol Int ; 47(5): 336-8, 1997 May.
Article in English | MEDLINE | ID: mdl-9143033

ABSTRACT

An epithelial ovarian tumor with steroid production was examined in a 70-year-old postmenopausal female. The stromal cells of this tumor were rather dense and occasionally characterized by luteinization or hyperthecosis, which has been associated with steroidogenesis. Subcellular visualization using confocal laser scanning microscopy (CLSM) successfully led to the identification of 3 beta-hydroxysteroid dehydrogenase (HSD) in both mitochondria-like small particles and endoplasmic reticulum-like linear profiles, and P450-aromatase also in endoplasmic reticulum-like linear profiles, on the three-dimensional images.


Subject(s)
Carcinoma/ultrastructure , Organelles/ultrastructure , Ovarian Neoplasms/ultrastructure , Steroids/biosynthesis , Aged , Carcinoma/metabolism , Female , Humans , Microscopy, Confocal , Organelles/metabolism , Ovarian Neoplasms/metabolism
15.
J Anal Toxicol ; 20(5): 318-22, 1996 Sep.
Article in English | MEDLINE | ID: mdl-8872242

ABSTRACT

A sensitive method for simultaneous determination of ester-type local anesthetic drugs (procaine, tetracaine, and T-caine) has been developed using wide-bore capillary gas chromatography with nitrogen-phosphorus detection (GC-NPD). The extraction procedure, the experimental conditions for heptafluorobutyryl (HFB) derivative formation, and the percentage of the ester-type local anesthetic drugs from the human serum are described. The HFB derivatives of ester-type local anesthetic drugs showed sensitivity of approximately 2-3 fold higher than that without derivatization. The detection limits of HFB derivatives of the ester-type local anesthetic drugs were approximately 60-70 pg on column. Recoveries from the human serum were 85-94%. This method could be used to determine concentrations as low as 24-28 ng/mliters of the ester-type local anesthetic drugs.


Subject(s)
Anesthetics, Local/analysis , Procaine/analysis , Tetracaine/analysis , para-Aminobenzoates , 4-Aminobenzoic Acid/analysis , 4-Aminobenzoic Acid/blood , Anesthetics, Local/blood , Humans , Nitrogen/chemistry , Phosphorus/chemistry , Procaine/blood , Sensitivity and Specificity , Solvents/chemistry , Tetracaine/blood
16.
Mech Ageing Dev ; 86(1): 27-37, 1996 Jan 05.
Article in English | MEDLINE | ID: mdl-8866734

ABSTRACT

The human red blood cells with phenotype En(a-) were characterized by the lack of MN antigens. The red blood cells with phenotype En(a-) which were found in a Japanese family were tested to clarify the changes in membrane surfaces of the red blood cells during in vivo ageing. The contents of sialic acid, glucose, mannose, galactose, fucose, N-acetylglucosamine and N-acetylgalactosamine of the red blood cell membranes obtained from the old red blood cells with phenotype En(a-) were significantly lower than those of the young red blood cell membranes. Neither the young nor the old red blood cells with phenotype En(a-) showed the agglutination with Arachis hypogaea (PNA) which was capable of binding to T agglutinogen. It is presumed that En(a-) red blood cells are not exposed to sialidase in vivo. In comparison with the young En(a-) red blood cell membranes, the number and the distribution density of lectin receptor sites on the old ones for Limulus polyphemus (LPA), Canavalia ensiformis (Con A), Triticum vulgaris (WGA) and Bauhinia purpurea (BPA) were significantly lower. It is thought that En(a-) red blood cell ageing is accompanied by elimination of some sialoglycoconjugates which have affinity for LPA, Con A, WGA and BPA, whereas En(a-) red blood cells lack glycophorin A.


Subject(s)
Erythrocyte Membrane/physiology , Erythrocytes/physiology , Adult , Carbohydrates/blood , Cellular Senescence , Centrifugation, Density Gradient , Erythrocytes/metabolism , Humans , Lectins/metabolism , Male , Peanut Agglutinin , Phenotype , Receptors, Mitogen/metabolism
17.
J Obstet Gynaecol (Tokyo 1995) ; 21(3): 305-12, 1995 Jun.
Article in English | MEDLINE | ID: mdl-8590371

ABSTRACT

OBJECTIVES: To clarify the relationship between the expression of GST-pi and the therapeutic effects of high-dose combination chemotherapy with support by autologous bone-marrow transplantation (ABMT). METHODS: Expression of GST-pi in tissue specimen were analyzed by indirect immunoperoxidase methods using anti-GST-pi polyclonal antibody. RESULTS: (1) Eighteen of 36 cases (50%) tested prior to administration of any chemotherapeutic agents were found to be positive in terms of immunohistochemical expression of GST-pi in the cancer cells. (2) The rate of GST-pi expression was elevated following administration of cisplatin, adriamycin, and cyclophosphamide, which suggests that administration of such anti-cancer agents is capable of inducing expression of GST-pi in cancer cells. (3) GST-pi negative patients exhibit better survival rates than do GST-pi positive patients (Kaplan Meier method: p < 0.001). CONCLUSIONS: Analysis of GST-pi obtained at primary surgery can serve as a valid marker in anticipating chemotherapeutic effects and long-term prognoses.


Subject(s)
Glutathione Transferase/metabolism , Ovarian Neoplasms/drug therapy , Ovarian Neoplasms/enzymology , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Female , Humans , Immunoenzyme Techniques , Ovarian Neoplasms/mortality , Ovarian Neoplasms/pathology , Prognosis , Survival Analysis , Treatment Outcome
18.
Free Radic Biol Med ; 18(6): 1003-12, 1995 Jun.
Article in English | MEDLINE | ID: mdl-7628726

ABSTRACT

The formation of phospholipid hydroperoxides was monitored in human red blood cell (RBC) membranes that had been peroxidized with an azo initiator. Peroxidation of RBC membranes caused a profound decrease in the amount of polyunsaturated fatty acids and concomitantly hydroperoxides, as primary products of peroxidation, appeared in the phospholipids. Hydroperoxides were predominantly generated in choline glycerophospholipid (CGP), while the extent of formation of ethanolamine glycerophospholipid (EGP) hydroperoxides was low and their presence was transient. Hydroxy and hydroperoxy moieties in CGP were identified as 9-hydroxy and 13-hydroxy octadecanoic acid, derived from linoleic acid, by gas chromatography-mass spectrometric analysis. No consistent generation of hydroperoxide from arachidonic acid was evident in CGP. The CGP-hydroperoxide accounted for approximately 76% of linoleic acid consumed during peroxidation of RBC membranes. The prominent generation of phospholipid hydroperoxides was observed in the linoleic acid-rich membranes from rabbit RBC, indicating that the level of linoleic acid in phospholipids determines, in part, the extent of formation of phospholipid hydroperoxides. Aldehydic phospholipids, as secondary products of peroxidation, were detected in oxidized membranes. EGP was the most prominent aldehydic phospholipid, while negligible amounts of aldehydic CGP were formed. This study indicates that the process of oxidation of individual phospholipids clearly differs among phospholipids and depends on the structure of each.


Subject(s)
Amidines/pharmacology , Erythrocyte Membrane/metabolism , Hydrogen Peroxide/blood , Linoleic Acids, Conjugated , Linoleic Acids/blood , Lipid Peroxidation/drug effects , Phosphatidylcholines/blood , Adult , Chromatography, High Pressure Liquid , Fatty Acids, Unsaturated/blood , Humans , Linoleic Acid , Phosphatidylethanolamines/blood
20.
Nihon Sanka Fujinka Gakkai Zasshi ; 46(10): 1041-8, 1994 Oct.
Article in Japanese | MEDLINE | ID: mdl-7814934

ABSTRACT

We investigated the relationship between the progression and regression of cervical dysplasia and the existence of HPV DNA. The materials we analyzed were 80 biopsied specimens obtained from 22 cases of dysplasia which were followed-up for a long time at our out-patient clinic, and 47 specimens of squamous cell carcinoma. Detection of HPV DNA was done by in situ hybridization with biotinylated HPV DNA probes types 6/11, 16/18 and 31/33/35. The analysis of several specimens obtained at long term follow-up showed that the dysplasia-progression group had a higher incidence and more frequent detection of HPV DNA than the regression group. In the progression group, HPV 16/18 were detected more frequently than HPV 31/33/35 at the dysplasia lesion, but HPV 6/11 was detected in neither the dysplasia nor the squamous cell carcinoma. Fourteen of the 47 cases of squamous cell carcinoma (29.8%) were positive for HPV DNA, and 13 of them (92.9%) were detected in the lesions of dysplasia adjacent to carcinoma in situ or invasive cancer. Comparison of the detection rate for HPV DNA was done in the 3 dysplastic areas, i.e. the dysplastic areas in the dysplasia progression group (A), dysplasia regression group (B) and in the area adjacent to squamous cell carcinoma (C). The detection rates for HPV DNA were 43.5% in A, 27.7% in C and 15.0% in B. From these results we drew the following conclusions: 1) The continuous existence of HPV DNA, especially type 16/18 in the dysplasia lesions, may progress from lesions to carcinoma in situ or invasive cancer.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
DNA, Viral/analysis , Papillomaviridae/genetics , Papillomavirus Infections/virology , Tumor Virus Infections/virology , Uterine Cervical Dysplasia/virology , Carcinoma, Squamous Cell/pathology , Carcinoma, Squamous Cell/virology , Disease Progression , Female , Follow-Up Studies , Humans , In Situ Hybridization , Papillomavirus Infections/pathology , Retrospective Studies , Tumor Virus Infections/pathology , Uterine Cervical Dysplasia/pathology , Uterine Cervical Neoplasms/pathology , Uterine Cervical Neoplasms/virology
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