1.
Chem Commun (Camb)
; 48(48): 6037-9, 2012 Jun 18.
Article
in English
| MEDLINE
| ID: mdl-22576748
ABSTRACT
Biotinylated amphipol was used to entrap FhuA (an E. coli outer membrane protein) and immobilize the FhuA-amphipol complex on streptavidin surfaces. Using this assembly, we have successfully devised surface-based assays for studying the recognition of FhuA by pb5 (a bacteriophage T5 protein) and determination of the affinity constant.