ABSTRACT
The levels of interferon-y (IFN-gamma), tumor necrosis factor-gamma (TNF-alpha), and interleukin-8 (IL-8) and their changes during chemotherapy and laser chemotherapy were studied in 97 patients with active infiltrative pulmonary tuberculosis, by taking into account Mycobacterium tuberculosis (MBT) sensitivity to chemical agents. The studies have indicated that the levels of IFN-gamma, TNF-alpha, and IL-8 are increased and may be markers of an active process. Chemotherapy causes a reduction in increased cytokinemia. Complex laser chemotherapy affects the cytokine profile in active tuberculosis caused by not only drug-sensitive, but also drug-resistant MBT.
Subject(s)
Cytokines/blood , Tuberculosis, Pulmonary/blood , Adolescent , Adult , Aged , Biomarkers/blood , Disease Progression , Female , Humans , Immunoassay , Male , Middle Aged , Severity of Illness Index , Tuberculosis, Pulmonary/diagnosis , Young AdultABSTRACT
The use of gas-liquid chromatography based on the determination of the fatty acid composition of bacterial cell makes it possible to rapidly identify Nocardia isolated from the patients' smears. Examining the fatty acid composition of museum and fresh Nocardia cultures suggests that they are largely homogeneous, The major fatty acids in Nocarida are hexadecenoic, octadecenoic, and tuberculostearic acids. Criteria for differentiating bacteria of the genus Nocarida via gas chromatographic analysis of their fatty acid spectrum from Mycobacteria and other nonmycobacterial acid-resistant microbes.
Subject(s)
Chromatography, Gas/methods , Fatty Acids/analysis , Nocardia/chemistry , Humans , Sensitivity and Specificity , Species SpecificityABSTRACT
By determination of fatty acid composition of bacterial cells, gas-liquid chromatography can quickly identify Nocardia microorganisms isolated from the infected material. The study of fatty acid spectrum of the reference and fresh cultures Nocardia has found their homogeneity. Such fatty acids as hexadecenoic, hexadecanoic, octadecenoic, octadecanoic and tuberculostearic acids occurred most frequently. Differential criteria are proposed for Nocardia microorganisms basing on gas-liquid chromatography data on fatty acid spectra from mycobacteria to other nonmycobacterial acid-fast microorganisms.