ABSTRACT
We studied the expression of activation markers CD25 and CD69 by blood lymphocytes in white mice vaccinated with Brucella abortus 19 BA in antigen-specific tests in vitro. During incubation of blood lymphocytes with brucellosis polysaccharide-protein antigen, a statistically significant increase in the expression of CD25 by B cells and CD69 by T cells was observed; brucellin increased the expression of CD25 by B and T cells. Comparative analysis of the action of antigen preparations B. abortus showed that only brucellin has antigen-specific activity against CD19+CD25+ cells. The used method can be considered as a promising test for evaluation of the effectiveness of brucellosis immunoprophylaxis, which substantiates the need for further research.
Subject(s)
Antigens, Bacterial/administration & dosage , B-Lymphocytes/drug effects , Brucella abortus/immunology , Brucellosis/immunology , T-Lymphocytes/drug effects , Animals , Antigens, Bacterial/immunology , Antigens, CD/genetics , Antigens, CD/immunology , Antigens, Differentiation, T-Lymphocyte/genetics , Antigens, Differentiation, T-Lymphocyte/immunology , B-Lymphocytes/immunology , B-Lymphocytes/microbiology , Brucellosis/diagnosis , Brucellosis/microbiology , Female , Flow Cytometry , Gene Expression , Immunophenotyping , Interleukin-2 Receptor alpha Subunit/genetics , Interleukin-2 Receptor alpha Subunit/immunology , Lectins, C-Type/genetics , Lectins, C-Type/immunology , Lymphocyte Activation/drug effects , Mice , T-Lymphocytes/immunology , T-Lymphocytes/microbiology , VaccinationABSTRACT
We studied immunotropic properties of synthetic selenium-organic preparation 2,6-dipyridinium-9-selenabicyclo[3.3.1]nonyl dibromide (974zh). The experimental preparation reduced the cAMP/cGMP ratio, which indicated an increase in proliferative activity of cells of immunocompetent organs (thymus and spleen) in experimental animals. It was shown that 974zh intensified the immune response to Yersinia pestis EV thereby increasing the resistance to the plague agent.
Subject(s)
Immunity, Innate/drug effects , Selenium Compounds/pharmacology , Adjuvants, Immunologic/chemistry , Adjuvants, Immunologic/pharmacology , Adjuvants, Immunologic/therapeutic use , Animals , Combined Modality Therapy , Cyclic AMP/metabolism , Cyclic GMP/metabolism , Female , Male , Mice , Organic Chemicals/chemistry , Organic Chemicals/pharmacology , Organic Chemicals/therapeutic use , Plague/drug therapy , Plague/immunology , Plague/prevention & control , Plague Vaccine/administration & dosage , Selenium/chemistry , Selenium/pharmacology , Selenium/therapeutic use , Selenium Compounds/chemistry , Selenium Compounds/therapeutic use , Spleen/drug effects , Spleen/immunology , Spleen/metabolism , Thymus Gland/drug effects , Thymus Gland/immunology , Thymus Gland/metabolism , Vaccine Potency , Virulence/drug effects , Yersinia pestis/drug effects , Yersinia pestis/immunology , Yersinia pestis/pathogenicityABSTRACT
We studied the effect of the organoselenium compound 2,6-dipyridinium-9-selenium-bicyclo[ 3,3,1]nonan dibromide (974zh) on the severity of pathological changes in the organs of experimental animals immunized with live tularemia and brucellosis vaccines. It was found that 974zh reduced reactogenicity of vaccines for experimental animals. Our findings indicate the prospects for further studies of the effects of 974zh on the functional state of experimental animals.
Subject(s)
Bacterial Vaccines/immunology , Brucellosis/immunology , Organoselenium Compounds/pharmacology , Synthetic Drugs/pharmacology , Tularemia/immunology , Animals , Magnetic Resonance Spectroscopy , MiceABSTRACT
The dynamics of LPO marker malondialdehyde formation and peroxidase-destroying activity was studied in homogenized organs of guinea pigs, immunized with thermoextracts from S and L forms Brucella abortus I-206. The L form brucella thermoextract exhibited a lower reactogenicity and adequately activated the antioxidant system, due to which the destructive effects of ROS could be partially neutralized during the vaccinal process.
Subject(s)
Animal Structures/drug effects , Antioxidants/metabolism , Brucella Vaccine/pharmacology , Brucella abortus/chemistry , Lipid Peroxidation/drug effects , Vaccines, Attenuated/pharmacology , Animal Structures/metabolism , Animals , Animals, Laboratory , Brucella Vaccine/chemistry , Brucella abortus/immunology , Brucella abortus/pathogenicity , Female , Guinea Pigs , L Forms/physiology , Liver/drug effects , Liver/metabolism , Liver/pathology , Male , Oxidative Stress/drug effects , Spheroplasts/physiology , Temperature , Vaccines, Attenuated/chemistryABSTRACT
There is evidence that the facultatively intracellular pathogen affects the functional capacity of phagocytes, which is associated with their bactericidal against a tularemic microbe with varying phenotypic properties. The tularemic microbe of the subspecies Francisella subsp. tularensis and F. tularensis subsp. mediaasiatica is shown to influence the phagocytic capacity of immunocompetent cells, resulting in incomplete phagocytosis. This is corroborated by the lower functional activity of neutrophils and macrophages in the interaction with tularemic microbe of the subspecies tularensis and mediaasiatica to a greater extent than in that with the representatives of other studied F. tularensis subspecies. The low content of cationic protein and the suppressed activity of NO-synthase may be associated with the loss of activity of the enzyme and cationic protein during phagocytosis of the tularemic microbe. The findings are of prognostic value in characterizing the severity of a pathological process caused by F. tularensis and in designing diagnostic and prophylactic agents.
Subject(s)
Francisella tularensis/pathogenicity , Tularemia/immunology , Tularemia/metabolism , Animals , Antimicrobial Cationic Peptides/metabolism , Francisella tularensis/classification , Guinea Pigs , Macrophages, Peritoneal/immunology , Macrophages, Peritoneal/metabolism , Neutrophils/immunology , Neutrophils/metabolism , Nitric Oxide Synthase/metabolism , Phagocytosis , VirulenceABSTRACT
Cholera epidemics in South and Central America in the period 1991-1999 are characterized. The mechanisms of existence of Vibrio eltor in environmental objects have been substantiated. The causes and conditions of endemic foci formation which determine the infection spread not only within the countries of the subcontinent, but also far beyond its borders are shown. The effectiveness of cholera control interventions is evaluated.
