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Prikl Biokhim Mikrobiol ; 20(3): 420-7, 1984.
Article in Russian | MEDLINE | ID: mdl-6205390

ABSTRACT

A technique for the quantitative determining of nucleic acids after electrophoresis by double-wave densitometry in UV spectrum by scanning agar gel along both axes was developed. Optical characteristics of agar and acrylamide-agar gels are given. The range of quantities measured is 6 divided by 30 micrograms of DNA per gel; the standard deviation adjusted to 1 microgram is less than +/- 3%.


Subject(s)
DNA/analysis , Densitometry/methods , Electrophoresis, Agar Gel/methods , Electrophoresis, Polyacrylamide Gel/methods , Electrophoresis/methods , RNA/analysis , Animals , Gels , Rats , Thymus Gland/analysis , Ultraviolet Rays
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