ABSTRACT
Extracellular concentration of heat shock protein (Hsp) with a molecular weight of 70 kDa (Hsp70) rapidly increases in the serum in response to stress and returns to the basal level during recovery. Further regulation of its blood concentration is unclear. A possible regulator is HspBP1, a protein binding Hsp70. Binding to ATPase domain of Hsp70, HspBP1 inactivates it, thus acting as a factor of nucleotide exchange. Blood sera from athletes were examined at the beginning and end of the last mesocycle of the training period by two-staged immunoaffinity test system. The concentration of HspBP1 increased with decreasing Hsp70 concentration under conditions of long-term training. Presumably, the dynamics of Hsp70 and HspBP1 concentrations can serve as the test for evaluating the adaptation potential.
Subject(s)
Adaptor Proteins, Signal Transducing/blood , HSP70 Heat-Shock Proteins/metabolism , Molecular Chaperones/metabolism , Physical Fitness/physiology , Adolescent , Exercise Test , HumansABSTRACT
The mechanisms of early activation of natural killers were studied in volunteers of different athletic qualification. The initial levels of expression of KIR2DL2, PRF1, and GZMB genes regulating the functions of natural killer differ in athletes and untrained subjects. Moderate exercise stimulates transcription activities of these genes. In athletes, the expression increases more intensely than in controls. Stimulation of inhibitory (KIR2DL3) and activation (KIR2DS2) receptors was revealed. This indicated nonspecific stimulation of natural killers, probably mediated by an increase in serum concentration of heat shock protein with a molecular weight of 70 kDa.
Subject(s)
Exercise , Receptors, KIR2DL3/genetics , Receptors, KIR/genetics , Receptors, Natural Killer Cell/metabolism , HumansABSTRACT
Study by means of expression profiling with Human GeneChip ST1.0 microchips (Affymetrix) revealed 10 early immune response genes, whose expression was modified after short-term intense physical exercise. They include genes for immunoglobulin-like receptors of natural killer cell, genes for IL-2Rß and IL-18RAP receptors, and two genes for functional proteins (perforin 1 and granzyme B) that provide the cytotoxic potential of killer cells. Possible mechanisms of stimulation, activation of the receptor apparatus, and cytotoxic effect on natural killer cells are evaluated under conditions of physical exercise.
Subject(s)
Exercise/physiology , Gene Expression Regulation/physiology , Receptors, Natural Killer Cell/metabolism , Gene Expression Regulation/immunology , Granzymes/metabolism , Humans , Interleukin-2 Receptor beta Subunit/metabolism , Male , Oligonucleotide Array Sequence Analysis , Perforin , Pore Forming Cytotoxic Proteins/metabolism , Receptors, Interleukin-18/metabolism , Receptors, Natural Killer Cell/genetics , Young AdultSubject(s)
Antineoplastic Agents/pharmacology , Doxorubicin/pharmacology , Drug Resistance, Neoplasm/genetics , Gene Expression Profiling , Lung Neoplasms/genetics , Lung Neoplasms/pathology , Biomarkers, Tumor/genetics , Cell Line, Tumor , Gene Expression Regulation, Neoplastic/drug effects , Gene Expression Regulation, Neoplastic/genetics , Humans , Signal TransductionABSTRACT
Association of brain processes presumably underlying aggression with serotonin transporter gene polymorphism in men was studied. Carriers of more active gene variant are characterized by higher aggression index, increased component of brain potential mismatch negativity responsible for automatic difference detection, and decreased P300 component characterizing involuntary attention and cognitive control.
Subject(s)
Aggression/physiology , Brain/physiology , Polymorphism, Genetic , Protein Isoforms/genetics , Serotonin Plasma Membrane Transport Proteins/genetics , Adolescent , Adult , Cognition/physiology , Electroencephalography , Evoked Potentials, Auditory/physiology , Humans , Male , Neuropsychological Tests , Protein Isoforms/metabolism , Serotonin Plasma Membrane Transport Proteins/metabolism , Young AdultABSTRACT
We studied the effect of angiotensin-converting enzyme gene polymorphism on human emotional state in humans (189 athletes and 212 volunteers not engaged in sport activity). The distribution of angiotensin-converting enzyme genotypes was estimated. The dependence of aggression on age, sex, and professional activity was evaluated. This polymorphism was associated with physical aggression in female synchronized swimmers. Physical aggression in II genotype carriers was lower than in D allele carriers. Our results indicate that individual differences in aggression depend on professional activity and are genetically determined.
