Cellulose membrane coated Mo-doped TiO2nanotube sheets for sustained oxidation of biomolecules.

Titanium dioxide nanotubes (TNT) are widely researched materials for the photocatalytic generation of free radicals, which are useful in wastewater treatment. We aimed to prepare Mo-doped TNT sheets, covered with a cellulose membrane to avoid TNT surface inactivation by protein adsorption. We studied the susceptibility of serum albumin (SA) bound to different molar ratios of palmitic acid (PA) to denaturation and fibrillation by this system, which is meant to mimic oxidative stress conditions such as non-alcoholic fatty liver disease. The results demonstrated that cellulose membrane-covered TNT successfully oxidized the SA, identified by structural changes to the protein. Increasing the molar ratio of PA to protein-enhanced thiol group oxidation while protecting the protein against structural changes. Finally, we propose that in this photocatalyzed oxidation system, the protein is oxidized by a non-adsorptive mechanism mediated by H2O2. Therefore, we suggest that this system could be used as a sustained oxidation system to oxidize biomolecules as well as potentially in wastewater treatment.

A New Mediterranean Flour Moth-Derived Chitosan: Characterization and Co-electrospun Hybrid Fabrication.

In this study, the chitin of adult Mediterranean flour moth (Ephestia kuheniella) (Cht) was extracted and then converted to chitosan by deacetylation process to achieve the chitosan derived from E. kuheniella (Chsfm). The new chitosan-based scaffold was produced using the polyvinyl alcohol (PVA) co-electrospinning technique. The degree of deacetylation was obtained using the distillation-titration and Fourier transform infrared spectroscopy. The surface morphology and crystallinity index of Chsfm were observed using scanning electron microscopy and X-ray diffraction analysis, respectively, and compared with the commercial chitosan (Chsc). Thermogravimetric analysis was used to estimate two chitosans' water content and thermal stability. The average molecular mass analysis was performed using viscometry. Moreover, the minimum inhibitory concentration and DPPH assay were used to study the antimicrobial activity and antioxidant potential of the Chsfm, respectively. Accordingly, Chsfm was smoother with fewer pores and flakes than Chsc, and its crystallinity index was higher than Chsc. The water content and thermal stability were lower and similar for Chsfm compared to Chsc. The average molecular mass of Chsfm was ~ 5.8 kDa, making it classified as low molecular weight chitosan. The antimicrobial activity of Chsfm against a representative Gram-negative bacteria; E. coli resulted to be the same as Chsc. However, less effective than Chsc against a representative Gram-positive bacteria is S. aureus. The Chsfm/PVA ratio scaffold was optimized at 30:70 to fabricate a uniform nanofiber scaffold.

Human serum albumin in neurodegeneration.

Serum albumin (SA) exists in relatively high concentrations, in close contact with most cells. However, in the adult brain, except for cerebrospinal fluid (CSF), SA concentration is relatively low. It is mainly produced in the liver to serve as the main protein of the blood plasma. In the plasma, it functions as a carrier, chaperon, antioxidant, source of amino acids, osmoregulator, etc. As a carrier, it facilitates the stable presence and transport of the hydrophobic and hydrophilic molecules, including free fatty acids, steroid hormones, medicines, and metal ions. As a chaperon, SA binds to and protects other proteins. As an antioxidant, thanks to a free sulfhydryl group (-SH), albumin is responsible for most antioxidant properties of plasma. These functions qualify SA as a major player in, and a mirror of, overall health status, aging, and neurodegeneration. The low concentration of SA is associated with cognitive deterioration in the elderly and negative prognosis in multiple sclerosis (MS) and amyotrophic lateral sclerosis (ALS). SA has been shown to be structurally modified in neurological conditions such as Alzheimer's disease (AD). During blood-brain barrier damage albumin enters the brain tissue and could trigger epilepsy and neurodegeneration. SA is able to bind to the precursor agent of the AD, amyloid-beta (Aß), preventing its toxic effects in the periphery, and is being tested for treating this disease. SA therapy may also be effective in brain rejuvenation. In the current review, we will bring forward the prominent properties and roles of SA in neurodegeneration.