ABSTRACT
Photodynamic therapy is the main technique applied for surface carcinoma treatment. This technique employs singlet oxygen generated via a laser excited photosensitizer as a main damaging agent. However, prolonged sensitivity to intensive light, relatively low tissue penetration by activating light the cost of photosensitizer (PS) administration can limit photodynamic therapy applications. Early was reported singlet oxygen generation without photosensitizer induced by a laser irradiation at the wavelength of 1250-1270 nm. Here, we study the dynamics of oxidative stress, DNA damage, changes of mitochondrial potential, and mitochondrial mass induced by a laser at 1265 nm have been studied in HCT-116 and CHO-K cells. Laser irradiation of HCT-116 and CHO-K cells has induced a dose-dependent cell death via increasing intracellular reactive oxygen species (ROS) concentration, increase of DNA damage, decrease of mitochondrial potential, and reduced glutathione. It has been shown that, along with singlet oxygen generation, the increase of the intracellular ROS concentration induced by mitochondrial damage contributes to the damaging effect of the laser irradiation at 1265 nm.
Subject(s)
Mitochondria/metabolism , Oxidative Stress , Photochemotherapy/adverse effects , Animals , CHO Cells , Cell Death , Cricetinae , Cricetulus , DNA Damage , Glutathione/metabolism , HCT116 Cells , Humans , Lasers , Low-Level Light Therapy/adverse effects , Membrane Potential, Mitochondrial , Mitochondria/radiation effects , Oxidation-Reduction , Singlet Oxygen/metabolismABSTRACT
A waveguide-saturable absorber with low propagation loss is fabricated by femtosecond pulses in YAG:Cr(4+) crystal. Q-switch operation of a Yb fiber laser with the new saturable absorber having absorption saturation parameters similar to the bulk YAG:Cr(4+) crystal is demonstrated.
