ABSTRACT
Histoplasma capsulatum is an infrequent but serious cause of endocarditis. The definitive diagnosis requires culture, which may require a long incubation. We demonstrated the ability of the Histoplasma capsulatum AccuProbe to accurately identify this organism when applied directly on an excised valve that contained abundant yeast forms consistent with H. capsulatum.
Subject(s)
DNA Probes , Endocarditis/diagnosis , Heart Valve Prosthesis/microbiology , Histoplasma/isolation & purification , Histoplasmosis/diagnosis , Aortic Valve , DNA, Ribosomal/analysis , Endocarditis/microbiology , Endocarditis/surgery , Histoplasma/classification , Histoplasma/genetics , Histoplasmosis/microbiology , Histoplasmosis/surgery , Humans , Male , Middle Aged , RNA, Ribosomal/genetics , Reagent Kits, DiagnosticABSTRACT
Mycobacterium triplex was first named in 1996 as an acid-fast bacillus with features that most resemble Mycobacterium simiae and Mycobacterium avium-intracellulare complex but which possesses a distinct mycolic acid pattern as well as a distinctive 16S rRNA hypervariable region. It has been isolated from lymph node, sputum, and cerebrospinal fluid specimens, but to date only rare clinical cases of this organism have been reported in the literature. The following is a case report of M. triplex that was isolated from the pericardial and peritoneal fluid of a 13-year-old female liver transplant patient.
