[Design of a chromosomal DNA probe species specific for Yersinia pestis]. / Konstruirovanie vidospetsifichnogo dlia Yersinia pestis khromosomnogo DNA-zonda.

A 14.8 kb DNA fragment from the chromosome of Yersinia pestis TWJ was cloned and the restriction map constructed. The fragment designated as T16 and its subfragments were tested in dot-hybridization with strains of Yersinia genus and other members of Enterobacteriaceae. A species-specific DNA probe (designated MK) was constructed on the basis of the T16 fragment. As judged from restriction analysis, blot-hybridization experiments and, partially, sequencing, significant homology exists between the MK DNA probe and this one developed by Bardarov et. al. (1990). A repeated sequence in two copies was discovered in the MK fragment.

[Alternative localization of the determinant of pathogenicity coded by the Yersinia pseudotuberculosis pVM82 plasmid]. / Al'ternativnaia lokalizatsiia determinant patogennosti, kodiruemykh plazmidoi pVM82 Yersinia pseudotuberculosis.

The chromosomal DNA regions in Yersinia pseudotuberculosis strains occur that are homologous to 25 Md DNA segment of the plasmid pVM82 encoding the bacterial capability of immunosuppression. The character of the chromosomal DNA regions dispersion reacting with the 25 Md segment probes is different in epidemiologically hazardous and nonvirulent strains of Yersinia pseudotuberculosis. The specific DNA regions occur as well as identical ones. The suppression of antibody formation to a number of main Yersinia pseudotuberculosis antigens by epidemiologically hazardous strain is demonstrated. The suppression is analogous to the one previously described for Yersinia pseudotuberculosis strains harbouring the plasmid pVM82.

[Structural-functional characteristics of Yersinia pseudotuberculosis plasmid pVM82]. / Strukturno-funktsional'naia kharakteristika plazmidy Yersinia pseudotuberculosis pVM82.

The restriction map of Yersinia pseudotuberculosis plasmid pVM82 was established using the "chromosome walking" method. According to transpositional mutagenesis, the plasmid pVM82 appeared to be conjugative and was able to be transmitted from Y. pseudotuberculosis to the E. coli K-12 cells.

[Integration with the chromosome--an alternative state of calcium-dependency plasmids in Yersinia]. / Integratsiia s khromosomoi--al'ternativnoe sostoianie plazmid kal'tsiizavisimosti u vozbuditelei iersiniozov.

Using the technique of the genes probes the naturally occuring strains Y. pseudotuberculosis and Y. enterocolitica of epidemiological importance were shown to contain pCad plasmid integrated with the chromosome. The strains having integrated plasmid express all pathogenicity determinants necessary for realization of infectious process.

[Localization of insertion into E. coli chromosome of Tn7-like transposons controlling resistance to trimethoprim and streptothricin]. / Lokalizatsiia vnedreniia v khromosomu E. coli Tn7-podobnykh transpozonov, kontroliruiushchikh ustoichivost' k trimetoprimu i streptotritsinu.

To localize the insertion sites for Tn7-like transposons Tn1824, Tn1825 and Tn1826 the EcoRI-cleaved fragments of E. coli recA+ and recA- strains chromosome carrying the transposons were hybridized. It was shown that transposition of Tn7-like elements takes place in the strictly defined region of E. coli chromosome, like it had been previously described for transposon Tn7.

[A new pathogenic trait encoded by Yersinia pseudotuberculosis pVM82 plasmid]. / Novyi priznak patogennosti, kodiruemyi plazmidoi pVM82 Yersinia pseudotuberculosis.

The strains of Yersinia pseudotuberculosis isolated from patients in the course of outbreaks of infection (epidemic strains) were found to possess at least two plasmids with molecular masses of 45 and 82 MD. In contrast, the strains obtained in sporadic cases harbored different sets of plasmids, but never the 82 MD plasmids. These plasmids designated pVM82 and isolated from strains of different geographic regions of the country were identical. pVM82 have no homology with Y. pestis plasmids of the similar size coding for the FraI antigen. The pVM82 DNA was found to be composed of the 57 MD plasmid DNA and the 25 MD fragment of Y. pseudotuberculosis DNA. Using Western blot hybridization technique it was shown that the presence of pVM82 suppressed formation of antibody against some major antigenic determinants of Y. pseudotuberculosis. Immunosuppression took place when the animals were infected with bacteria grown below 20 but not at 37 degrees C. The 57 MD plasmid failed to produce immunosuppression. It was concluded that the 25 MD fragment of pFN82 encoded a novel pathogenic factor responsible for immunosuppression.