ABSTRACT
DNA typing of nine short tandem repeat (STR) loci was carried out on unrelated Israeli Jewish and Arab individuals. All loci were highly polymorphic and the distribution of the obtained genotypes did not deviate from Hardy-Weinberg equilibrium. A comparison between Jewish and Arab population data revealed statistically significant differences in allele frequency distributions for some of the loci. The results presented in this study enable the use of these nine STR loci for forensic, identification and paternity cases in the Jewish and the Arab populations of Israel.
Subject(s)
Arabs/genetics , Gene Frequency , Jews/genetics , Tandem Repeat Sequences/genetics , Computer Simulation , DNA Fingerprinting , Forensic Medicine/methods , Humans , IsraelABSTRACT
Chorionic villus sampling (CVS), prior to pregnancy termination (pre-termination CVS), is suggested as a tool for forensic paternity testing. Unlike the abortion material, which consists of ruptured tissues of fetal and maternal origin, extra-embryonic membranes obtained through CVS can provide an uncontaminated source of fetal tissue for genotyping. We discuss the possibility of confined placental mosaicism (CPM) and its implications on the polymerase chain reaction (PCR) based analyses of short tandem repeats (STRs) and the D1S80 loci.
Subject(s)
Abortion, Legal , Chorionic Villi Sampling , Paternity , Rape , Adolescent , Alleles , Child Abuse, Sexual , DNA/analysis , Female , Genotype , HLA-DQ Antigens/analysis , Humans , Male , Minisatellite Repeats/genetics , Polymerase Chain Reaction , PregnancyABSTRACT
Pre-transfusion and post-transfusion blood samples from eight individuals were typed at 10 PCR amplified loci. In no case did the PCR DNA profile of the post-transfusion blood sample differ from that of the pre-transfusion profile.
