ABSTRACT
The antibiotic resistance group B streptococcal plasmids, pIP501 and pVA797, were conjugally transferred from Streptococcus faecalis to Lactobacillus plantarum. The Escherichia coli-Streptococcus shuttle plasmids, pVA838 and pSA3, were mobilized from S. sanguis to L. plantarum by pVA797 via cointegrate formation. pVA838 readily resolved from pVA797 and was present in L. plantarum as deletion derivatives. The pVA797::pSA3 cointegrate failed to resolve in L. plantarum.
Subject(s)
Conjugation, Genetic , Lactobacillus/genetics , R Factors , Streptococcus/genetics , Transfection , DNA, Bacterial/analysis , Electrophoresis, Agar Gel , Enterococcus faecalis/genetics , Escherichia coli/genetics , Genetic Vectors , Nucleic Acid Hybridization , Streptococcus agalactiae/genetics , Streptococcus sanguis/geneticsABSTRACT
The streptococcal plasmid pAM beta 1 (erythromycin resistance) was transferred via conjugation from Streptococcus faecalis to Lactobacillus plantarum and was transferred among L. plantarum strains. Streptococcus sanguis Challis was transformed with pAM beta 1 isolated from these transconjugants, and transformants harboring intact pAM beta 1 could conjugate the plasmid back to L. plantarum.
