Ethnomedicine of Dharwad district in Karnataka, India--plants used in oral health care.

The present ethnomedicine survey covers the Dharwad district of Karnataka in southern India. It was revealed that 35 plants belonging to 26 families are being used to treat different types of oral ailments like toothache, plaque and caries, pyorrhea and aphthae. Sixteen of these plants were new claims for the treatment of oral ailments not previously reported in the ethnomedicinal literature of India. Basella alba, Blepharis repens, Capparis sepiaria, Oxalis corniculata and Ricinus communis are used for the treatment of aphthae; Azima tetracantha, Caesalpinia coriaria, Cleome gynandra, Gossypium herbacium, Leucas aspera, Merremia chryseides, Pergularia daemia, Prosopis juliflora and Solanum nigrum are used to treat tooth ache and Cassia hirsuta and Cassia tora are used in the treatment of plaque and caries.

Ethnomedicobotany of Uttara Kannada District in Karnataka, India--plants in treatment of skin diseases.

Present paper deals with the herbal remedies for skin diseases in Uttara Kannada district of Karnataka--a Southern State in India, which is located in the hearts of Western Ghats. In an ethnobotanical exploration of this area 52 herbal preparations from 31 plants belonging to 21 families have been recorded. This also includes 17 new claims to the ethnomedical knowledge. The parts used and methods of preparation are discussed along with the family and local name for all the plants.

Ethnomedical knowledge of plants used by Kunabi Tribe of Karnataka in India.

Therapeutic effects and medicinal efficacy of the wild herbs were identified and administered by the tribal people to cure various ailments. Recently, the practice of herbal medicine has been diminishing, which may lead to the loss of valuable information about healing herbs. The Uttara Kannada District of Karnataka in India is one of the rich biodiversity centers of Western Ghats. Many tribes like Gowlis, Siddis, Halakki Okkaligas and Kunabis inhabit the semi-evergreen forests of the District. The mixed marathi speaking Kunabis have migrated from Goa and settled here a long time back and are even today without modern facilities. Thus, in the way of revitalization of traditional herbal medicines, it is an effort to record the valuable ethnomedical knowledge of the tribe Kunabis of Uttara Kannada District in Karnataka. A total of 45 species of plants used by Kunabi community people are described here based on an ethnomedical field survey. These plants belong to 26 families and are used to treat a wide range of discomforts like fever, cough, skin diseases, rheumatism, snakebite, jaundice, dysentery, etc.

Triacontanol inhibits both enzymatic and nonenzymatic lipid peroxidation.

The effect of the plant growth regulator, triacontanol (TRIA) on lipid peroxidation was studied in three different systems: (i) isolated chloroplasts of spinach (Spinacea oleracea L.) leaves; (ii) egg lecithin liposomes; and (iii) soybean lipoxygenase (LOX) system. The nonenzymatic lipid peroxidation in isolated chloroplasts and egg lecithin liposomes was measured as the amount of thiobarbituric acid reactive substances (TBARS) formed. Inhibition of Fe2+ and/or light-induced lipid peroxidation by TRIA was observed in both isolated chloroplasts and egg lecithin liposomes. The kinetics of soybean lipoxygenase-1 (LOX-1) was studied using linoleic acid as the substrate. The enzyme was competitively inhibited by TRIA. The Ki for TRIA inhibition of the enzyme was estimated to be 3.2-5.0 microM according to different methods of estimation. TRIA has been known to exhibit anti-inflammatory action in animals and this anti-inflammatory effect of TRIA might be mediated through inhibition of lipid peroxidation. Since LOX inhibitors have been extensively used as therapeutic agents, TRIA, being a natural compound has been suggested to be an effective anti-inflammatory drug.

Microviscosity of cucumber (Cucumis sativus L.) fruit protoplast membranes is altered by triacontanol and abscisic acid.

Cucumber (Cucumis sativus L.) fruit protoplast membranes were probed with diphenylhexatriene (DPH) and pyrene, and also with two different plant growth regulators, triacontanol (TRIA) and abscisic acid (ABA). Fluorescence anisotropies of DPH and pyrene were measured after incorporating them into the membranes. The fluorescence lifetime of membrane-bound pyrene was also measured by using neodymium-doped yttrium aluminium garnet (Nd:YAG) laser of 35 ps pulses. The microviscosities of the membranes were calculated using the values of fluorescence anisotropy and lifetime. In the presence of TRIA and ABA, there was a sharp decrease in the fluorescence lifetime of pyrene. Similarly, there was also a decrease in the microviscosities of the membranes and increase in the rate of rotation of membrane-bound fluorophore, induced by the plant growth regulators. Furthermore, TRIA or TRIA + ABA could reduce the fluorescence anisotropy of both the fluorophores whereas, ABA decreased the anisotropy of only pyrene. This property of ABA may be due to its confinement to a specific spacial facet in the membrane. Fatty acid analysis indicated that membrane microviscosity fluctuations were not due to altered fatty acid composition alone as it is known that change in lipid-protein interaction would also alter the physical status of the membrane.