1.
Zhong Yao Cai
; 29(5): 448-9, 2006 May.
Article
in Chinese
| MEDLINE
| ID: mdl-16981455
ABSTRACT
OBJECTIVE: To determine the content of hypoxanthine in Pheretima aspergillum from different habitats. METHOD: A RP-HPLC method was established. The chromatographic column was Inertsil ODS-EP. The mobile phase was H2O-CH3OH-C4H8O(93:: 7: 0.05). The flow rate was 1.0 ml/min, and the detection wavelength was 254 nm. RESULTS: The average recoveries for hypoxanthine was 98.6% , precision of the method was 0. 50% (RSD, n = 6). CONCLUSION: The method can be used to determine the content of hypoxanthine in Pheretima aspergillum from diffrent habitats.