ABSTRACT
OBJECTIVE: To explore the difference of expression of proteins between the serum and hippocampus after brain injury in rats. METHODS: Male SD rats were used to establish brain injury model. The changes of proteins expression profile in serum and hippocampus at different time after brain injury were analyzed using weak cationic exchanger (WCX2) chips and immobilized metal affinity capture arrays-Cu (IMAC-Cu) chips by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. RESULTS: A total of 436 protein peaks were detected in serum and 346 protein peaks were detected in hippocampus using WCX2 chips. A total of 229 protein peaks were detected in serum and 345 protein peaks were detected in hippocampus using IMAC-Cu chips. The same 10 protein peaks were respectively detected in serum and hippocampus using WCX2 chips. The same 13 protein peaks were respectively detected in serum and hippocampus using IMAC-Cu chips. CONCLUSION: The changes of protein expression profile in serum and hippocampus are obvious after closed brain injury and show a significant difference. The different proteins detected in serum and hippocampus using the same chip could be biochemical markers for determining brain injury.
Subject(s)
Head Injuries, Closed/metabolism , Hippocampus/metabolism , Protein Array Analysis/methods , Proteins/metabolism , Animals , Blood Proteins/analysis , Brain/metabolism , Brain/pathology , Disease Models, Animal , Head Injuries, Closed/blood , Head Injuries, Closed/diagnosis , Male , Predictive Value of Tests , Proteins/analysis , Proteomics , Rats , Rats, Sprague-Dawley , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
OBJECTIVE: To observe the surface ultrastructure of different tumor cells in vivo using atomic force microscope (AFM) and analyze their common characteristics. METHODS: We selected 60 specimens of each of normal liver cells, liver cancer, cervical squamous cells, cervical cancer cells, ductal epithelial cells and breast cancer cells for scanning using AFM. The cell surface scan images were analyzed using image analysis software to identify their common morphological features. RESULTS: From normal cervical squamous epithelial cells, intermediate cells, and basal cells to HPV-infected cells, CIN2-3 cells and cervical cancer cells, the membrane surface roughness became gradually increased (P<0.05). Similarly, the surface roughness increased significantly in the order of normal liver cells, hepatitis B cirrhosis liver cells, and hepatocellular carcinoma cells (P<0.05). The average surface roughness also tended to increase from normal mammary gland cells to mammary gland hyperplasia cells and breast cancer cells (P<0.05). CONCLUSION: Normal cells and tumor cells show different cell membrane morphologies, and such morphological features provide a reliable basis for clinical pathological diagnosis and differential diagnosis of malignancies.
Subject(s)
Breast Neoplasms/ultrastructure , Liver Neoplasms/ultrastructure , Microscopy, Atomic Force , Uterine Cervical Neoplasms/ultrastructure , Epithelial Cells/ultrastructure , Female , Hepatocytes/ultrastructure , Humans , Image Processing, Computer-Assisted , Male , Membrane Proteins/ultrastructureABSTRACT
OBJECTIVE: To study the alteration in cortex protein fingerprinting of cerebral cortex after closed brain injury in rat. METHODS: Seventy-two male Sprague-Dawley (SD) rats were randomly divided into sham operation group, 4, 8, 12, 24 and 48 hours postinjury groups. Eight rats in each group were used for WCX-2 protein chip research, and 4 rats in each group for pathological examination. Marmarou's weight-dropping model was reproduced, and brain cortex was harvested for study with hematoxylin-eosin (HE) staining, and Bradford method was adopted for WCX-2 protein chip research, and protein chip reading was obtained for protein fingerprinting analysis. RESULTS: (1) The pathological observation showed different degree of injury could be seen in all the injury groups. (2) The WCX-2 experiment found that 3 protein expressions had changed in cortex after brain injury compared with the sham operation group. The differential protein with molecular weight of 5,639 protein expression was found to be upregulated at 8 hours after injury (P<0.05). The 3,212 protein did not expressed in sham operation or 4, 8, 12 and 24 hours groups, but upregulated at 48 hours after injury (P<0.05). The expression of 7,536 protein was upregulated at 24 hours after injury (P<0.05), but not in sham operation or 4, 8, 12, and 48 hours groups. CONCLUSION: Alterations in protein expression in cerebral cortex could be induced after brain injury.
Subject(s)
Brain Injuries/metabolism , Cerebral Cortex/metabolism , Protein Array Analysis , Proteins/metabolism , Animals , Brain Injuries/pathology , Cerebral Cortex/pathology , Disease Models, Animal , Male , Rats , Rats, Sprague-DawleyABSTRACT
AIM: To explore the alteration of serum protein fingerprinting of rats subjected brain injury. METHODS: Male Sprague-Dawley rats were randomly divided into seven groups, ie, normal control group, sham operation group, injury 4 h, 8 h, 12 h, 24 h and 48 h groups. The change of protein pattern in serum was monitored with weak cationic exchanger chip and surface-enhanced laser desorption ionization-time of flight-mass spectrometry. RESULTS: The reduction of 5648 Da protein expression was detected at 4 h, 8 h or 12 h after injury (P < 0.01, compared with control and sham operation) and recovery at 24 h or 48 h. The increase of 9681 Da protein expression was detected at 4 h, 8 h or 12 h after injury (P < 0.05, compared with control and sham operation) and recovery at 24 h or 48 h. CONCLUSION: The alteration of protein expression in serum could be induced after brain injury.
