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1.
Zhong Yao Cai ; 37(1): 66-9, 2014 Jan.
Article in Chinese | MEDLINE | ID: mdl-25090707

ABSTRACT

OBJECTIVE: To study the chemical constituents of Paeonia lactiflora flowers. METHODS: [corrected] The chemical constituents were isolated and purified by various chromatography methods,and the structures were identified by physicochemical and modem spectroscopic. RESULTS: 11 compounds were identified as gallic acid(1),methyl gallate(2),ethyl gallate(3),1,2,3,6-tetragalloyl-beta-D-glucopyranoside(4), 1,2,3,4,6-pentagalloyl-beta-D-glucopyranoside(5), quercetin-3-O-glucoside-6"-gallate(6), kaempferol-3-O-glucoside-6"-gallate(7), 1-O-galloyl-beta-D-glucose (8), kaempferol-3, 7-di-O-beta-D-glucoside(9), paeoniflorin(10) and albiflorin(11). CONCLUSIONS: Compounds 1-8, 10 and 11 are obtained from the flowers of Paeonia lactiflora for the first time,compounds 6 and 7 are obtained from Paeonia genus for the first time.


Subject(s)
Drugs, Chinese Herbal/chemistry , Flowers/chemistry , Paeonia/chemistry , Plants, Medicinal/chemistry , Bridged-Ring Compounds/chemistry , Bridged-Ring Compounds/isolation & purification , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal/isolation & purification , Glucosides/chemistry , Glucosides/isolation & purification , Kaempferols/chemistry , Kaempferols/isolation & purification , Molecular Structure , Monosaccharides/chemistry , Monosaccharides/isolation & purification , Monoterpenes/chemistry , Monoterpenes/isolation & purification
2.
J Sep Sci ; 36(16): 2680-5, 2013 Aug.
Article in English | MEDLINE | ID: mdl-23784883

ABSTRACT

This study aimed to seek an efficient method to extract and purify yunaconitine and 8-deacetylyunaconitine from Aconitum vilmorinianum Kom. by accelerated solvent extraction combined with pH-zone-refining counter-current chromatography. The major extraction parameters for accelerated solvent extraction were optimized by an orthogonal test design L9 (3)(4). Then a separation and purification method was established using pH-zone-refining counter-current chromatography with a two-phase solvent system composed of petroleum ether/ethyl acetate/methanol/water (5:5:2:8, v/v) with 10 mM triethylamine in the upper phase and 10 mM HCl in the lower phase. From 2 g crude extract, 224 mg of 8-deacetylyunaconitine (I) and 841 mg of yunaconitine (II) were obtained with a purity of over 98.0%. The chemical structures were identified by ESI-MS and (1)H and (13)C NMR spectroscopy.


Subject(s)
Aconitine/analogs & derivatives , Aconitum/chemistry , Countercurrent Distribution/methods , Plant Extracts/isolation & purification , Aconitine/chemistry , Aconitine/isolation & purification , Molecular Structure , Plant Extracts/chemistry , Spectrometry, Mass, Electrospray Ionization
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