ABSTRACT
Ten different pig breeds with different genetic origin (they are eight Chinese Domestic breeds, one recently Developed breed and one Introduced breed respectively) were utilized in this study to analyze the phylogeny of MyoG gene. 63 individuals were sequenced to get their complete nucleotide sequences of MyoG gene. Different methods and software were used and 6 phylogenetic trees were got based on the complete nucleotide sequence. To test the validation of these phylogenetic trees, the topologic structure differences between different trees were compared. The results indicate that the Neighbour Joining tree of the MyoG gene (Figure 2, NJ tree) has the best topological structure. In this NJ tree, 6 Chinese Domestic breeds cluster in one branch, Dahe and Wuzhishan pig constructed a new branch, Dahewu pig, Duroc and Standard MyoG gene sequence (Genbank accession number X89007) are clustered in one branch, the results are concord with the breeding history of these breeds and can reflect their true genetic background. Same topologic structure and parameters were found between different phylogenetic trees got by different methods. The same parameters of these trees confirmed the fact that the MyoG gene is very conservative and there are very few mutation sites between different breeds.
Subject(s)
Myogenin/genetics , Myogenin/metabolism , Animals , China , Computational Biology/methods , Gene Frequency , Models, Genetic , Mutation , Oligonucleotides/genetics , Phylogeny , Polymerase Chain Reaction , Sequence Analysis, DNA , Software , Species Specificity , SwineABSTRACT
We obtained the complete mitochondrial genome of U.thibetanus mupinensis by DNA sequencing based on the PCR fragments of 18 primers we designed. The results indicate that the mtDNA is 16,868 bp in size, encodes 13 protein genes, 22 tRNA genes, and 2 rRNA genes, with an overall H-strand base composition of 31.2% A, 25.4% C, 15.5% G and 27.9% T. The sequence of the control region (CR) located between tRNA-Pro and tRNA-Phe is 1422 bp in size, consists of 8.43% of the whole genome, GC content is 51.9% and has a 6bp tandem repeat and two 10bp tandem repeats identified by using the Tandem Repeats Finder. U. thibetanus mupinensis mitochondrial genome shares high similarity with those of three other Ursidae: U. americanus (91.46%), U. arctos (89.25%) and U. maritimus (87.66%).
