ABSTRACT
OBJECTIVE@#To investigate the expression and significance of miRNA-324-3p and its target gene WNT2B in tissue specimens of nasopharyngeal carcinoma (NPC) specimens.@*METHOD@#qRT-PCR was used to detect the expression of miRNA-324-3p and WNT2B mRNA, and Western blot was applied to assay the expression of WNT2B protein in 39 cases of NPC specimens and 21 cases of non-carcinoma epithelium. The relationship between their expression levels and clinicopathological characteristics and their correlation with clinical pathological parameters was analyzed.@*RESULT@#The expression of miRNA-324-3p was significantly down-regulated decreased but WNT2B mRNA/protein increased obviously in NPC specimens (P < 0.01). A negative correlation between miRNA-324-3p and WNT2B was spotted (P < 0.05). The expression levels of these markers were closely correlated with T stage, clinic stage and cervical lymph node metastasis (P < 0.05).@*CONCLUSION@#The loss of miRNA-324-3p and ectopic WNT2B might co-induce the initiation and progression of NPC.
Subject(s)
Humans , Carcinoma , Glycoproteins , Genetics , Metabolism , Lymphatic Metastasis , MicroRNAs , Metabolism , Nasopharyngeal Carcinoma , Nasopharyngeal Neoplasms , Metabolism , Neoplasm Proteins , Metabolism , RNA, Messenger , Metabolism , Wnt Proteins , Genetics , MetabolismABSTRACT
Purpose The aim is to immobilize the thrombin and to increase the stability of immobilized enzyme.Methods Thrombin was immobilized on chitosan and crosslinked with glutaraldehyde, and the optimum conditions for immobilization and stability of immobilized enzyme were studied.Results This preparation of immobilized thrombin was practicable,and the stability of this immobilized enzyme was obviously improved than that of thrombin.Conclution The conditions for immobilization were good, and reliable.Stability of immobilized thrombin to light, high temperature,low temperature and observation on reserving at room temperature was good.
