Electrowetting: A Consideration in Electroadhesion.

With the commercialization of haptic devices, understanding behavior under various environmental conditions is crucial for product optimization and cost reduction. Specifically, for surface haptic devices, the dependence of the friction force and the electroadhesion effect on the environmental relative humidity and the finger hydration level can directly impact their design and performance. This article presents the influence of relative humidity on the finger-surface friction force and the electroadhesion performance. Mechanisms including changes to Young's modulus of skin, contact angle change and capillary force were analyzed separately with experimental and numerical methods. Through comparison of the calculated capillary force in this paper and the electroadhesion force calculated in published papers, it was found that electrowetting at high voltage could contribute up to 60% of the total friction force increase in electroadhesion. Therefore, in future design of surface haptic devices, the effect of electrowetting should be considered carefully.

The Application of Tactile, Audible, and Ultrasonic Forces to Human Fingertips Using Broadband Electroadhesion.

We report an electroadhesive approach to controlling friction forces on sliding fingertips which is capable of producing vibrations across an exceedingly broad range of tactile, audible, and ultrasonic frequencies. Vibrations on the skin can be felt directly, and vibrations in the air can be heard emanating from the finger. Additionally, we report evidence from an investigation of the electrical dynamics of the system suggesting that an air gap at the skin/surface interface is primarily responsible for the induced electrostatic attraction underlying the electroadhesion effect. We developed an experimental apparatus capable of recording friction forces up to a frequency of 6 kHz, and used it to characterize two different electroadhesive systems, both of which exhibit flat force magnitude responses throughout the measurement range. These systems use custom electrical hardware to modulate a high frequency current and apply surprisingly low distortion, broadband forces to the skin. Recordings of skin vibrations with a laser Doppler vibrometer demonstrate the tactile capabilities of the system, while recordings of vibrations in the air with a MEMS microphone quantify the audible response and reveal the existence of ultrasonic forces applied to the skin via electronic friction modulation. Implications for surface haptic and audio-haptic displays are briefly discussed.

Examining the occurrence of residues of flunixin meglumine in cull dairy cows by use of the flunixin cull cow survey.

OBJECTIVE: To determine whether cull dairy cows with signs of certain clinical conditions, termed suspect, are more likely than healthy-appearing cull dairy cows to have violative concentrations of flunixin meglumine in their tissues at slaughter. DESIGN: Cross-sectional study. ANIMALS: 961 cull dairy cows. PROCEDURES: Suspect cull dairy cows were selected from 21 beef slaughter establishments with a high production volume of dairy cows, and kidney and liver tissues were collected for screening. Kidney tissues were screened for antibiotics and sulfonamides with the fast antimicrobial screening test (FAST). Liver tissues were screened for flunixin meglumine with an ELISA, and quantitative analysis of ELISA-positive samples was performed with high-performance liquid chromatography. During the same time period, liver tissues from 251 healthy-appearing cull dairy cows were collected for the Food Safety and Inspection Service National Residue Program Scheduled Sampling Plan, but were screened only for flunixin meglumine. RESULTS: Of 710 suspect cull dairy cows, 50 (7.04%) had liver tissue flunixin concentrations higher than the flunixin tolerance concentration (0.125 ppm). Thirty-one of 168 (18.45%) FAST-positive and 19 of 542 (3.51%) FAST-negative suspect cull dairy cows had violative tissue flunixin concentrations. Two of the 251 (0.80%) healthy-appearing cull dairy cows had violative tissue flunixin concentrations. CONCLUSIONS AND CLINICAL RELEVANCE: Suspect cull dairy cows, especially those that were also FAST positive, had a significantly higher incidence of violative tissue flunixin concentrations than healthy-appearing cull dairy cows at slaughter. Targeted sampling plans for flunixin meglumine in suspect dairy cows can help to support more efficient use of resources and further safeguard the nation's food supply.

Comparison of screening methods for antibiotics in beef kidney juice and serum.

Rapid screening tests can be used as part of an efficient program designed to monitor veterinary drug residues in cattle. In this work, three rapid tests designed to screen samples for the presence of antibiotic residues, the Fast Antimicrobial Screen Test (FAST), Premi and Kidney Inhibition Swab (KIS) tests, were compared using beef kidney juice and serum samples. In order to provide a realistic assessment, potentially incurred samples of beef kidney juice and serum were obtained from 235 carcasses which had been retained by inspectors in a processing plant for further testing. In addition, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was conducted on these samples to identify what antibiotics were present, if any, and their levels. The comparison of the three rapid screening test results with those from LC-MS/MS analysis allowed for a more complete comparison of the relative sensitivity of these analytical methods, as well as valuable information on false positive and negative response rates.

Use of bovine single nucleotide polymorphism markers to verify sample tracking in beef processing.

OBJECTIVE: To determine whether a selected set of 20 single nucleotide polymorphism (SNP) markers derived from beef cattle populations can be used to verify sample tracking in a commercial slaughter facility that processes primarily market (ie, culled) dairy cows. DESIGN: Prospective, blinded validation study. ANIMALS: 165 cows and 3 bulls from 18 states (82% Holstein, 8% other dairy breeds, and 10% beef breeds). PROCEDURE: Blood was collected by venipuncture from randomly chosen animals just prior to slaughter. The purported corresponding liver samples were collected during beef processing, and genotype profiles were obtained for each sample. RESULTS: On the basis of SNP allele frequencies in these cattle, the mean probability that 2 randomly selected individuals would possess identical genotypes at all 20 loci was 4.3 x 10(-8). Thus, the chance of a coincidental genotype match between 2 animals was 1 in 23 million. Genotype profiles confirmed appropriate matching for 152 of the 168 (90.5%) purported blood-liver sample pairs and revealed mismatching for 16 (9.5%) pairs. For the 16 mismatched sample pairs, 33% to 76% of the 20 SNP genotypes did not match (mean, 52%). Discordance that could be attributed to genotyping error was estimated to be < 1% on the basis of results for split samples. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that this selected set of 20 bovine SNP markers is sufficiently informative to verify accuracy of sample tracking in slaughter plants that process beef or dairy cattle. These or similar SNP markers may facilitate high-throughput, DNA-based, traceback programs designed to detect drug residues in tissues, control of animal diseases, and enhance food safety.