[Photodynamic damage of yeast subcellular fraction induced by elevated levels of endogenous protoporphyrin IX]. / Fotodinamicheskoe povrezhdenie subkletochnykh struktur drozhzhei s indutsirovannym nakopleniem éndogennogo protoporfirina IX.

The 2,2'-dipyridyl-induced accumulation of protoporphyrin IX in Saccharomyces cerevisiae cells was shown to be accompanied by the photoinhibition of cell respiration and the enhancement of the photoinduced permeability of plasma membranes to the fluorescent dye primuline. The visible-light illumination (at 400-600 nm) of the mitochondria and plasma membranes isolated from yeast cells with a high level of endogenous protoporphyrin IX intensified lipid peroxidation in these subcellular organelles. Comparative studies showed that the rad 52 mutant cells, which are deficient in the postreplicative recombinational DNA repair system, are considerably more sensitive to the inactivating action of visible light than are the wild-type cells and the rad 3 mutant cells, which are deficient in the excision DNA repair system. The contribution of photodynamic damage to the yeast subcellular organelles to the lethal photodynamic effect is discussed.

[Fluorescent photobleaching of endogenous protoporphyrin IX in yeast cells]. / Fotovytsvetanie fluorestsentsii éndogennogo protoporfirina IX v drozhzhevykh kletkakh.

Yeast Saccharomyces cerevisiae accumulate in the presence of chelator 2,2'-dipyridyl, two fractions of photosensitizer protoporphyrin IX, which fluoresce with maxima at 625 and 635 nm. The two fractions were found to differ from each other in the dynamics and character of fluorescence photobleaching. In contrast to the protoporphyrin IX that fluoresces with a maximum at 625 nm, the protoporphyrin IX fraction that fluoresces with a maximum at 635 nm is more photolable; in addition, upon photobleaching of its fluorescence, a new maximum at 675 nm appears.

Endogenous porphyrin accumulation and photosensitization in the yeast Saccharomyces cerevisiae in the presence of 2,2'-dipyridyl.

The chelator 2,2'-dipyridyl (0.2 mM) induces a remarkable increase of protoporphyrin IX concentration as well as of its Zn-containing complex in the yeast Saccharomyces cerevisiae. Endogenous porphyrin accumulation results in five- to six-fold cell sensitization to visible light (400-600 nm). Mitochondria isolated from the cells grown in the presence of 2,2'-dipyridyl accumulate protoporphyrin IX and Zn-protoporphyrin IX, while plasma membranes besides that exhibit porphyrin-type fluorescence at 670-675 nm in chloroform extract. The protoporphyrin IX content increases more than four-fold in mitochondria and two-fold in plasma membranes isolated from chelator-treated cells. The relative contribution of subcellular structure photodestruction to photoinduced cell inactivation is discussed.