[NADPH-diaphorase topochemistry in human and rat knee joint structures and its changes in experimental arthritis]. / Topokhimiia NADPH-diaforazy v strukturakh kolennogo sustava cheloveka i krys i ee izmeneniia pri éksperimental'nom artrite.

Using the histochemical demonstration of NADPH-diaphorase (NADPH-d) activity, the distribution of constitutive nitric oxide synthase (NOS) activity was studied in the cartilage and synovium of healthy man and rats and in experimental arthritis. Arthritis was induced in rats by the injection of complete Freund's adjuvant; the material was studied at days 7, 14, 30 and 60 of inflammation. NADPH-d activity was different in man and rat. In human cartilage NADPH-d-positive chondrocytes were absent, whereas in synovium synovial lining cells and endothelial cells of microvessels were marked. In rats of control group and in those treated with adjuvant, high activity of the enzyme was demonstrated in the cytoplasm of proliferating chondroblasts situated in the marginal zone of the cartilage, and in chondrocytes, that formed the isogenous groups in its deep portions. In rat synovium, besides synovial lining and endothelial cells, fibroblasts were also stained. At experimental days 7 and 14 the number of the marked cells with predominantly high enzyme activity increased on the average by approximately 30.4%. This parameter decreased significantly, approaching the control level at day 30, while at day 60 of adjuvant arthritis NADPH-d positive cells were not detected. Heterotopic localization of constitutive NOS indicates that NO is unequally involved in arthritis development in man and rat. The dynamics of enzyme activity depends on the stage of inflammation and determines the specific effect of NO on target cells.

[Effect of epidural morphine analgesia on the NO-forming activity of nociceptive neurons of the spinal ganglia and bone marrow]. / Vliianie épidural'noi anal'gezii morfinom na NO-obrazuiushchuiu aktivnost' notsitseptivnykh neironov spinal'nykh gangliev i spinnogo mozga.

The effect of epidural morphine analgesia on NO mechanisms was studied during the primary and secondary sensitizations. The nitro-oxide synthase (NOS) activity was evaluated by the method of Home and Vincent, 1989, (identification of NADPH diaphorase). NOS and NADPH diaphorases are two double enzymes in cells with a synchronously changing activity. The NOS activity is in line with the level of the NO secretion. The enzyme was measured on a microdensitometer. The findings evidence an active involvement of NO mechanisms in the formation of primary and secondary hyperalgesia, and the injection of morphine caused a significant decrease in the count of NO-expressing neurons in the total number of cells with high and average activities of the enzyme.

[Effect of epidural morphine anesthesia on NO-ergic activity of nociceptive neurons of spinal ganglia and spinal cord]. / Vliianie épidural'noi analgezii morfinom na NO-obrazuiushchuiu aktivnost' notsitseptivnykh neironov spinal'nykh gangliev i spinnogo mozga.

The effect of epidural morphine anesthesia on NO-ergic mechanisms was studied during primary and secondary sensitization. Nitro-oxide synthase (NOS) activity was evaluated by the method of Home and Vincent (1989) (identification of NADPH diaphorase). NOS and NADPH diaphorase are two double enzymes in cells with synchronously changing activity. NOS activity corresponds to the level of NO secretion. The enzyme was measured on a microdensitometer. The findings evidence active involvement of NO-ergic mechanisms in the formation of primary and secondary hyperalgesia, and injection of morphine caused a significant decrease in the count of NO-expressing neurons in the total number of cells with high and average activities of the enzyme.

[Nitric oxidergic neurons of the respiratory organs]. / Nitrooksidergicheskie neirony organov dykhaniia.

Using histochemical NADPH-diaphorase reaction NO-ergic neurons distribution in human tracheobronchial tree was studied in man and mammals. Psedounipolar sensory neurons forming microganglia mark in adventitia of the trachea posterior surface from superior border up to trachea bifurcation. It was suggested that protoneurons are the constant structural formations of respirator organs. Their capacity to exert effector influence on the tissue through axon-reflex mechanism, apart from their sensory function is under discussion.

[Nitric oxide production by normal and injured neurons of Nodose ganglion and Nucleus dorsalis of Vagus nerve]. / Obrazovanie oksida azota normal'nymi i povrezhdennymi neironami uzlovatogo gangliia i dorsal'nogo iadra bluzhdaiushchego nerva.

Nodosum ganglion and nucleus dorsalis contain from 19.2 to 22.65% of NO-positive neurons. Their amount increases up to almost 77.4 and 68.8% in the ganglion and the nucleus dorsalis, respectively, in the damaged nervus vagus due to i-NOS transcription. It has been shown that NO participates in desorganization and recovery of the traumatic neuron, as auto- and paracrine regulator.

[Staged treatment and follow-up of patients with yersinia infections]. / Etapnoe lechenie i dispanserizatsiia bol'nykh iersiniozami.

Basing on the data obtained on immunopathogenetic and lipid metabolism features of intestinal yersiniosis and pseudotuberculosis in children, the authors offer variants of etiotropic and pathogenetic treatment specific for certain stages of the above diseases which are able to reduce the rate of aggravations, recurrences and complications by 10-12%. Long-term follow-up showed that unfavourable outcomes of yersiniosis are not infrequent this urging the necessity of 6-12-month follow-up of the convalescents.