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[Bacterial luciferase in reactions with catalytically reduced flavin mononucleotide]. / Bakterial'naia liutsiferaza v reaktsiiakh s kataliticheski vosstanovlennym flavinmononukleotidom.

Shumikhin, V N; Danilov, V S; Egorov, N S.

Biofizika ; 26(1): 130-2, 1981.

Article in Russian | MEDLINE | ID: mdl-7225439

Subject(s)

Flavin Mononucleotide , Luciferases , Photobacterium/enzymology , Aldehydes , Catalysis , Luminescence , Oxidation-Reduction , Oxygen

[Isolation and purification of bacterial luciferase from Photobacterium fischeri for analytical purposes]. / Vydelenie i ochistka bakterial'noi liutsiferazy iz Photobacterium fischeridlia analiticheskih tselei.

Shumikhin, V N; Danilov, V S; Malkov, Iu A; Egorov, N S.

Biokhimiia ; 45(9): 1576-81, 1980 Sep.

Article in Russian | MEDLINE | ID: mdl-7248358

ABSTRACT

A procedure resulting in a highly purified preparation of bacterial luciferase with a high specific activity towards FMNH2 and NADH was developed. Using SDS-electrophoresis in polyacrylamide gel, it was shown that the enzyme has a subunit composition and that its monomers do not contain the luciferase activity. The use of the obtained preparation for determining the content of NADH and FMN by the bioluminescent method allowed to increase its sensitivity up to 10(-18) and 10(-17) moles, respectively.

Subject(s)

Luciferases/isolation & purification , Photobacterium/enzymology , Flavin Mononucleotide , Kinetics , Luciferases/metabolism , Luminescent Measurements , NAD , Oxidation-Reduction

[Use of the method of the ion-exchange paper precipitation of protein receptors to estradiol from the cytosol of the rat hypothalamus and uterus for the study of their physicochemical characteristics]. / Ispol'zovanie metoda osazhdeniia belkov-retseptorov k éstradiolu iz tsitozola gipotalamusa i matki krys na ionoobmennoi bumage dlia izucheniia ikh fiziko-khimicheskikh kharakteristik.

Peryshkova, T A; Shishkina, I V; Shumikhin, V N.

Lab Delo ; (9): 542-7, 1978.

Article in Russian | MEDLINE | ID: mdl-81344

Subject(s)

Estradiol/isolation & purification , Hypothalamus/analysis , Receptors, Estrogen/isolation & purification , Uterus/analysis , Animals , Chemical Phenomena , Chemistry, Physical , Chromatography, DEAE-Cellulose/methods , Cytosol/analysis , Female , Rats

Subject(s)

ABSTRACT

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