Subject(s)
Bacterial Vaccines/immunology , Neoplasms, Experimental/prevention & control , Neoplasms/prevention & control , Animals , Bacteria/immunology , Bacterial Vaccines/therapeutic use , Humans , Immunotherapy/methods , Neoplasms/immunology , Neoplasms/therapy , Neoplasms, Experimental/immunology , Neoplasms, Experimental/therapySubject(s)
Immunotherapy/trends , Neoplasms/therapy , Adjuvants, Immunologic/therapeutic use , Animals , Antigens, Neoplasm/immunology , BCG Vaccine/therapeutic use , Burkitt Lymphoma/therapy , Cell Transformation, Neoplastic/immunology , Colonic Polyps/genetics , Female , Humans , Immunization , Laryngeal Neoplasms/therapy , Neuroblastoma/prevention & control , Ovarian Neoplasms/genetics , Papilloma/therapyABSTRACT
It has been shown that the accumulation of lipids and maturation of L strain cells into mature lypocytes can be stimulated by high doses (30-60 mg/ml) of serum albumins, egg albumin lysozyme, and gelatin, or with low doses (200-600 mcg/ml) of serum globulins. The globulins are believed to induce differentiation of the L cell on depressing their proliferation. On the contrary, the low doses of albumins stimulate cell division without inducing differentiation.
Subject(s)
Blood Proteins/pharmacology , L Cells/drug effects , Adipose Tissue/cytology , Adipose Tissue/drug effects , Animals , Cattle , Cell Differentiation/drug effects , Dose-Response Relationship, Drug , Humans , L Cells/cytology , Mice , Serum Albumin/pharmacology , Serum Albumin, Bovine/pharmacology , Serum Globulins/pharmacologyABSTRACT
In a medium supplemented with 60 per cent large cattle serum, part of the cell population of L-transformed culture was seen to be differentiated into mature lipocytes. The formation of structures similar to normal fat tissue took place in the same conditions, provided a reduced quantity of the seeded cells was used. The cell differentiation was accompanied with a decrease in acid phosphatase and non-specific esterase activities. The ultrastructural study has demonstrated a suitable preservation of cytoplasmic structures and a domination of the rough cytoplasmic reticulum compared to the control cell cultures where free ribosomes and polysomes predominated.