ABSTRACT
Experimental modeling of oxidative stress was conducted by creating circulatory brain hypoxia, accompanied by development of free radical processes leading to genome instability through an example of anterior epithelial layer of rat cornea. We studied the influence of infrared low-level laser radiation (ILLLR) in the therapeutic dose and mode (pulse power 8 W, λ=0.89 µm). Exposure of animals with circulatory brain hypoxia (ischemia) to ILLLR reduced the level of chromosome aberrations in corneal epitheliocytes and led to change in the mitotic index. Correction of the damaging effects of ischemia in the experiment in the absence of mutagenic effect of ILLLR in therapeutic dose and mode of exposure indicates the possibility of applying this physical method for correcting after-effects of oxidative stress during progression of degenerative eye diseases in ophthalmic practice.
Subject(s)
Cornea , Hypoxia, Brain , Animals , Cornea/radiation effects , DNA Damage , Hypoxia, Brain/radiotherapy , Lasers , Oxidative Stress/radiation effects , RatsABSTRACT
The physiological stress modeled by circulatory hypoxia activates LPO processes in various tissues. In posthypoxic period, the infrared low-intensity laser irradiation significantly decreased the chemiluminescence parameters in blood plasma, normalized the retinal levels of diene and triene conjugates, and decreased MDA in the rat brain attesting to the correcting effect of this irradiation during various types of physiological stresses.
Subject(s)
Hypoxia/radiotherapy , Infrared Rays/therapeutic use , Lipid Peroxidation/radiation effects , Phototherapy , Retina/radiation effects , Animals , Antioxidants/metabolism , Antioxidants/radiation effects , Free Radicals/metabolism , Free Radicals/radiation effects , Hypoxia/metabolism , Hypoxia/pathology , Laser Therapy/methods , Lasers , Male , Oxidative Stress/physiology , Oxidative Stress/radiation effects , Phototherapy/methods , Rats , Retina/metabolismABSTRACT
The results of the screening study of the acuity of schoolchildren between 7 and 17 years old living in Rostov Region of the Russian Federation are discussed in the article. The method of computer optometry was used to measure acuity. 93772 pupils, 48621 girls and 45151 boys, from 150 schools participated in this examination. It was found that there is sustained growth of those with low acuity (0,2 and less) among children of both sexes from junior group (7 years) to the senior (17). The signs of the decline in visual acuity among girls (14%) are manifested earlier than in boys (8%). The decline in visual acuity among 7-8-year-old children is about 3%. While comparing children from rural areas with those from big cities a true dependence of the parameter (acuity) on the factors (city and sex) was found.
Subject(s)
Learning/physiology , Sex Factors , Visual Acuity/physiology , Adolescent , Child , Female , Humans , Male , Russia , Urban PopulationABSTRACT
Influence of infrared cold laser emission (IRCL) on the dynamic equilibrium between lipid peroxidation and tension of the antioxidant defense system in rat's tissues (blood, brain, retina, cornea) was evaluated in animals with circulatory cerebral hypoxia induced by occlusion of the left carotid artery. Tissues of white rats were examined for IRCL effects on hemiluminescence, malonic dialdehyde, SOD and catalase activities on the background of circulatory cerebral hypoxia. Data of the experiment evidenced an antioxidant effect of posthypoxic IRCL therapy as it reduces intensity of the free radical processes in plasma, cerebral tissues and retina. The experiment demonstrated the IRCL ability to modulate LPO, to stiffen the antioxidant defense system in the event of eye diseases originated from circulatory hypoxia of the ocular analyzer.
Subject(s)
Brain/metabolism , Cerebrovascular Circulation/radiation effects , Cornea/metabolism , Free Radical Scavengers/metabolism , Hypoxia, Brain/radiotherapy , Low-Level Light Therapy/methods , Retina/metabolism , Animals , Brain/radiation effects , Cornea/radiation effects , Disease Models, Animal , Disease Progression , Free Radical Scavengers/radiation effects , Hypoxia, Brain/metabolism , Male , Rats , Retina/radiation effectsABSTRACT
This work was designed to study effect of low-intensity infrared laser radiation (LIIRR) on orbital circulation in children with progressive progressive short-handedness. Parameters of the blood flow in the orbital artery, central retinal artery, and posterior ciliary artery were evaluated in a total of 88 patients at the age from 6 to 14 years. The study group (group 1) comprised 66 children with moderately severe progressive myopia (112 eyes), the control one (group 2) included 22 patients without visual disturbances (44 eyes). Patients of group 1 received physiotherapy for the treatment of significantly deteriorated hemodynamics in the orbital region. Indirect action of LIIRR on the orbital region promoted stabilization of short-handedness in 78.8% of the treated patients. It is concluded that the method described in this paper is clinically efficient and may be recommended for the prevention of progressive myopia in children.
Subject(s)
Infrared Rays/therapeutic use , Low-Level Light Therapy/methods , Myopia/radiotherapy , Orbit/blood supply , Regional Blood Flow/radiation effects , Adolescent , Child , Diagnostic Techniques, Ophthalmological , Female , Humans , Male , Myopia/diagnosis , Myopia/etiology , Orbit/radiation effects , Treatment OutcomeABSTRACT
Effects of infrared cold laser radiation (IRCLR) on mutagenesis and proliferation of the corneal epithelium were studied with laboratory white mice subjected to instigated circulatory hypoxia of the brain. The experiment was to reveal whether IRCLR influences the frequency of chromosomal rearrangements and to allow calculation of the corneal cells mitotic index for circulatory brain hypoxia. Laser radiation was shown to reconstitute the normal frequency of chromosomal aberrations as well as the mitotic cycle in epithelial cells of the mice cornea. Data of the experiment are promising from the standpoint of antihypoxic use of IRCLR in ophthalmology.
Subject(s)
Brain Ischemia/genetics , Chromosome Aberrations/radiation effects , Epithelium, Corneal/radiation effects , Low-Level Light Therapy/methods , Mutagenesis/radiation effects , Animals , Brain Ischemia/pathology , Brain Ischemia/radiotherapy , Epithelium, Corneal/pathology , Male , RatsABSTRACT
The paper deals with the impact of infrared low-intensity laser radiation (IRLILR) on a mutation process and the proliferative activity of the animal cornea during stimulation of circulatory brain hypoxia. During an experiment on laboratory albino rats, IRLILR was studied for its impact on the level of chromosomal rearrangements and the mitotic index in the corneal cells was calculated in circulatory brain hypoxia. Laser exposure during stimulation of circulatory brain hypoxia favors normalization of the level of chromosomal aberrations and a mitotic cycle in the rat corneal epithelial cells. The experimental findings suggest that IRLILR may be used in ophthalmological care for antihypoxic purposes.
Subject(s)
Cornea/pathology , Corneal Diseases/radiotherapy , Hypoxia, Brain/complications , Low-Level Light Therapy/methods , Mutation/radiation effects , Oxidative Stress/genetics , Animals , Cell Proliferation/radiation effects , Chromosome Aberrations/radiation effects , Cornea/radiation effects , Corneal Diseases/etiology , Corneal Diseases/genetics , Disease Models, Animal , Hypoxia, Brain/genetics , Hypoxia, Brain/metabolism , Male , Oxidative Stress/radiation effects , Rats , Treatment OutcomeABSTRACT
In order to determine the eyeball shape, the authors have carried out ultrasonic biometry of its three major parameters, the anteroposterior axis (APA), horizontal diameter (HD), and vertical diameter (VD), and estimated the ratios of these values (APA/HD and APA/VD) in children with emmetropia (234 eyes) and those with slight and medium-grave myopia (660 eyes), aged 7 to 14. The findings evidence a compressed ellipsoidal shape of the eyeball, presenting as a vertical oval, in all subjects with emmetropic refraction, whatever their age. In myopia the eyeball shape transforms, and all the eyeball sizes are increased, but the APA size is growing more rapidly than the rest sizes, and the eyeball acquires the ball shape with a trend to an elongated ellipsoidal shape. The mean APA length in 7-14-year-old children with emmetropia was up to 23 +/- 0.15 mm, whereas in those with the ball shape of the eyeball it was distended.
