[Cytophilic immunoglobulins on the surface of neutrophilic granulocytes in mice perorally immunized with a live vaccine made from the supressor revertant S. typhimurium Rev 8]. / Tsitofil'nye immunoglobuliny na poverkhnosti neitrofil'nykh granulotsitov u myshei, immunizirovannykh peroral'no zhivoi vaktsinoi iz supressornogo revertanta S. typhimurium Rev 8.

The reaction of the spleen cell migration inhibition in the presence of monospecific antisera against mouse G, A and M immunoglobulins was used to detect cytophilic antibodies on the surface of mouse granulocytes. The oral administration of ACR live vaccine from suppressor revertant Salmonella typhimurium Rev. 8 protected the mice against infection induced by virulent species of mouse. Salmonella typhimurium. The immunized mice showed an increase in cytophilic IgG on the surface of neutrophile granulocytes.

[Morphological changes using live vaccine from the suppressor S. typhimurium revertant in model experiments]. / Morfologicheskie ismeneniia pri ispol'zovanii zhivoi vaktsiny iz supressornogo revertanta S. typhimurium v model'nykh opytakh

The work of was aimed at the morphological study of the harmlessness and protective activity of the live salmonella vaccine prepared form the suppressor S. typhimurium revertant intended for the prophylaxis and treatment of carrier state after the sustained food poisoning. It was shown to model experiments on mice that oral immunization caused no development of pathological changes, and at the same time prevented the appearance of the disease in infection of the animals by the virulent strain--S. typhimurium No. 415 in a dose of 100 LD50. A marked immunomorphological reaction developed in the small intestine and the lymphoid organs.

[Experimental study of the stability of avirulent properties and the immunogenicity of suppressor revertants of streptomycin-dependent salmonella mutants]. / Eksperimental'noe izuchenie stabil'nosti avirulentnykh svoistv i immunogennosti supressornykh revertantov streptomitsinzavisimykh mutantov sal'monell

The authors aimed at the experimental confirmation of avirulent properties of suppressor revertants of streptomycin-dependent salmonella mutants and also at the study of their immunogenicity. Stability of the loss of virulence by the suppressor revertants was established by studying the virulence of the subcultures isolated from them, differing by the genotype. Repeated passage of suppressor revertants of S. typhimurium Rev8, S. enteritidis Rev5, S. pullorum Rev through the biological objects highly sensitive to salmonella infection failed to lead to elevation of the virulence of the cultures under study. Live salmonella vaccines prepared from the suppressor revertants proved to be highly immunogenic in single oral and parenteral immunization of albino mice and chicks. Analogous immunization with killed vaccines was much less effective, streptomycin-dependent vaccines occupying the intermediate position.

[Live enteric typhoid vaccine consisting of a Vi-negative double-dependent mutant of S. typhi and a Vi-positive strain of Citrobacter]. / Eksperimental'nye issledovaniia zhivoi énteral'noi vaktsiny protiv briushnogo tifa, sostoiashchei iz Vi-negativnogo dvuzavisimogo mutanta S. typhi i Vi-pozitivnogo shtamma Citrobacter

The authors present experimental data on the study of the living enteral vaccine against the typhoid infection from the Vi-negative strain of salmonella with a double-dependence by streptomycin and purine, and from the Vi-positive strain -- citrobacter 5396/38. The method of immunoelectrophoretic analysis showed an indenticity of the O- and H-antigens of the doubledependent mutant with the O- and H-antigens of the typhoid strains of bacteria (Ty2-4446 and 5501). A sufficiently marked immunological reaction was revealed in the tests of antibody formation and in the study of the preventive activity of the sera of the immunized rabbits. The efficacy of the enteral immunization with the associated vaccine consisting of a doubledependent mutant of typhoid bacilli and the citrobacter strain in the doses tested (a 6-fold immunization) was demonstrated in experiments on albino mice. Association in one preparation of the cultures under study did not lead to any changes in the immunogenic properties of these strains. The cells of the mutant strain administered per os gave a positive culture (from the mouse organism) only in the course of the first 24 hours, in difference from the citrobacter strain which gave a positive culture in the course of 14 days.

[Influence of mutation in the gene-suppressor su-str on the virulence of Salmonella enteritidis]. / Vliianie mutatsii v gene-supressore su-str na virulentnost' Salmonella enteritidis

Virulent and avirulent cells resistant to streptomycin were revealed in the population of avirulent streptomycin-resistant mutant (obtained from the supressor revertant str-d S. enteritidis mutant) in seeding on a medium with streptomycin. Transduction analysis of the isolated avirulent and virulent strains demonstrated that mutation in the gene-suppressor su-str+ led to the loss of virulence. In connection with the presence in the genom of suppressor revertants of the streptomycin-dependent mutants two mutations (su-su+ and str-d), which irrespective of one another led to the loss of virulence, a conclusion was drawn on the stability of avirulent properties of such strains and on future prospects for their use in the capacity of living vaccines.