ABSTRACT
The impact of various methods of plasty, using net implants, on results of umbilical hernias treatment was studied in experimental and clinical investigation. The umbilical hernias plasty was performed in accordance to the IPOM (intraperitoneal on lay mesh) method, application of which have permitted to reduce a hospital stay of the patients as well as their period of social rehabilitation, and to guarantee the best cosmetic effect in comparison with such while making umbilical hernias plasty in accordance to a sub lay method.
Subject(s)
Abdominal Cavity/surgery , Hernia, Umbilical/surgery , Herniorrhaphy/methods , Surgical Mesh , Abdominal Cavity/pathology , Adult , Animals , Female , Hernia, Umbilical/pathology , Hernia, Umbilical/rehabilitation , Herniorrhaphy/instrumentation , Humans , Interleukin-1/blood , Interleukin-10/blood , Interleukin-6/blood , Length of Stay/statistics & numerical data , Male , Materials Testing , Pain Measurement , Pain, Postoperative/blood , Pain, Postoperative/physiopathology , Rabbits , Shear Strength , Tensile Strength , Tumor Necrosis Factor-alpha/bloodABSTRACT
The aim of the study is to examine in the experiment on the animals the mechanical properties of connective tissue complexes formed in alloplasty area using the intraperitoneal on lay mesh and sublay methodologies with further comparison of them. The experiment has been conducted on 12 rabbits of Russian chinchilla breed. Animals were distributed in the following way: the first group--operated by intraperitoneal on lay mesh methodology (n = 6) through implantation of composite grid Proceed with one-side celullose coating produced by "Ethicon" company. The second group--performed modeling of preperitoneal plastic using two-component composite grid with large-pores Ultrapro produced by "Ethicon" company (n = 6). For the tensometric evaluation of the strength of implant integration into the red wall was used entire area of anterior red wall together with the implanted transplant. According to the deflection diagrams and dynamometer rates defined the maximum burden rates which is equivalent of muscular tissue budge against polymeric matrix. Statistically significant distinctions during 14 days were not detected; however strength during 30 days in the first group was 3 times higher than in the second group. We consider that the methodology of intraperitoneal on lay mesh can be considered as operation of choice of surgery treatment of the umbilical hernias.
Subject(s)
Abdominal Wall/surgery , Connective Tissue/surgery , Hernia, Ventral/surgery , Surgical Mesh , Tensile Strength , Allografts/physiology , Animals , Cellulose/chemistry , Female , Male , Materials Testing/instrumentation , Porosity , RabbitsABSTRACT
Bioluminescent analysis has been made of the effect of oxygen supply on the content of ATP in the isolated olfactory epithelium of the frog. It was shown that storage of epithelium preparations in the air increases their ATP content. When preparations are kept in the atmosphere of an inert gas, ATP level in the epithelium rapidly decreases, being recovered after transition of preparations to the air medium. The data obtained indicate the existence of apical type of respiration in the olfactory epithelium of the frog.
Subject(s)
Olfactory Bulb/metabolism , Oxygen Consumption , Rana temporaria/metabolism , Adenosine Triphosphate/metabolism , Animals , Epithelium/metabolism , In Vitro Techniques , Time FactorsABSTRACT
The distribution of ATP in different parts of the frog olfactory organ was investigated. It is demonstrated that the highest macroerg amount is characteristic of the receptor part of olfactory mucosa. The amount of ATP decreased after the stimulation of the olfactory mucosa by odorants. The role of ATP in olfactory receptor process is discussed.
Subject(s)
Adenosine Triphosphate/metabolism , Olfactory Bulb/metabolism , Acetates/pharmacology , Animals , Camphor/pharmacology , Histocytochemistry , In Vitro Techniques , Microscopy, Electron, Scanning , Mucus/metabolism , Odorants , Olfactory Bulb/drug effects , Olfactory Bulb/ultrastructure , Rana temporaria , Stimulation, ChemicalABSTRACT
Generation of electric (delta psi) and chemical (delta pH) components of electrochemical proton gradient delta muH+, in plasma membrane vesicles of Heracleum sosnovskyi phloem cells was investigated. ATP-dependent generation of delta psi at pH 6.0 in the presence of Mg2+ and K+ was established with the help of fluorescent probes AU+ and ANS-. Protonophore CCCP and proton ATPase inhibitor DCCD suppressed generation, whereas oligomycin, the inhibitor of mitochondrial ATPases did not affect it. Measurings of delta psi value indicated its oscillations within the limits from 10 to 60 mV. ATP-dependent generation of delta pH was established by means of fluorescent probe 9-AA. The effect was eliminated by CCCP and stimulated by K+, that may testify to the transformation of a part of delta psi into delta pH at antiport H+/K+. Existence of H+-ATPase in the plasma membranes of higher plant cells insuring generation of delta muH+ is supposed.
