[Effect of apparatus plasmapheresis on the bowel barrier and motility function in patients with acute necrotizing pancreatitis].

Influence of therapeutic plasmapheresis on bowel barrier function and evacuation was investigated in 83 patients with severe acute necrotizing pancreatitis. Except standard therapy patient obtained therapeutic plasmapheresis using "Haemonetics" PCS 2 system. Complex treatment of patients with acute necrotizing pancreatitis and dynamic ileus using plasmapheresis increases contractive and propulsive function of stomach and duodenum and prolongs period of activity of these organs on 32%. Intestinal barrier function associates with restoration of bowel evacuation. Addition of plasmapheresis to standard therapy of necrotizing pancreatitis can be effective prevention of dynamic ileus.

[Bile composition in patients with chronic pancreatitis].

There was investigated a hepatic bile in 50 persons, aged 35-58 years old, including 20--practically healthy persons (I group), 20 patients, suffering chronic fibrose-degenerative pancreatitis (CHFDP) without jaundice syndrome (II group) and 10 patients, suffering CHFDP with jaundice syndrome (III group). There were determined the contents of the bile acids, the lipids and electrolytic contents of bile. A trustworthy difference in the bile contents was registered in patients, suffering CHFDP with the jaundice syndrome and without it, comparing with such in healthy persons. This have had permitted to add the complex of medicinal preoperative preparation of these patients substantially, and to apply the electrolytes content of a bile to apply as an additional diagnostic marker.

[Expediency of the liposuction in surgery of abdominal surgery, abdominal diseases and small organs of the pelvis]. / Tselesoobraznost' osushchestvleniia dermatolipéktomii pri vypolnenii operatsii po povodu gryzhi zhivota, zabolevanii organov briushnoi polosti i malogo taza.

The experience of performance of dermatolipectomy in 124 patients while conducting operative intervention for abdominal hernia and for the abdominal cavity and the small pelvis organs diseases was summarized. The risk degree of the simultant operation performance was estimated while conducting the proper preoperative preparation and the surgical correction volume was planned beforehand. The simultant operation performance did not influence the stationary treatment duration significantly, it also permitted to reduce the duration of temporary disability to shorten significantly the treatment expenses, to stop the stress influence on patient's psychological state caused be the reoperation conduction necessity. Low-molecular heparin fraxiparin (produced by Sanofi firm) was applied for the thromboembolic complications prophylaxis under control of the blood coagulation time and of the coagulogramm indices.

[Nucleotide sequence of the fragment of transcriptionally active locus in rat genome and analysis of a putative protein product]. / Nukleotidnaia posledovatel'nost' uchastka transkriptsionno aktivnogo lokusa K51 genoma krysy i analiz predpolagaemogo belkovogo produkta.

Part of the transcriptionally active rat genomic locus, K51, has been sequenced (2,321 b.p.). The coding DNA chains was identified and two long open reading frames (ORF) were found by computer analysis one of which seems to be much less probable. By transcriptional mapping, at least two exons (A - about 110 b.p., B - 549 b.p.) were revealed. Computer search showed that the deciphered nucleotide and amino acid sequence was absent in the data-banks up to the end of 1988. Numerous GC rich direct repeats were noticed in the fragment. At protein level, resemblance with hypervariable N- and C-terminal domains of cytokeratins was visualized. Some regions of the putative protein product possess relatedness with some other gene products, particularly with ATP binding domains of mos oncoprotein.

[Evolutionary variants of the mos gene]. / Evoliutsionnye varianty gena mos.

The occurence of members of mos oncogene family in the vertebrates genome has been studied with the help of highly labeled single-stranded DNA probes. These included subgenic v-mos clones as well as the unique sequence--specific recombinants from mos-related human locus gp5 and the K51 locus from rat genome. The probe from gp5 (mos pseudogene) interacts only with DNA of primates and of rodents. On the other hand, mos gene and the gene from K51 locus are present in all vertberates tested. Recent duplication of the main mos gene in Artiodactyla and Perissodactyla orders of mammals was identified. The persistence of K51 and mos genes during evolution indicates their importance. The segregation of three mos-related genes in human-hamster hybrids points to their location on different human chromosomes.