[Detection of antigen-antibody interaction of human adenovirus by the method of surface plasmon resonance].

A possibility to detect adenoviral protein--hexon, using specific antibodies by surface plasmon resonance (SPR) was demonstrated. The hexon of the human adenovirus 2 (Ad2) binds to antibodies immobilized on the sensor surface treated by KNCS and protein A Staphylococcus aureus. The specificity of antihexon antibodies was demonstrated by indirect method of fluorescent antibodies (MFA) and cellular variant of the immunoassay (cELISA).

[Immunospecific detection of the potato virus X by the surface plasmon resonance].

Highly specific and proximal method of laboratory diagnostics of the viral disease, has been developed using the structural protein of the potato virus X, as a model, and monospecific antibodies to it. The immunospecific determination of the potato virus X was carried out by the surface plasmon resonance method using specific IgG-antigen complexes, immobilized on the sensor surface modified by rodanide and protein A of Staphylococcus aureus.

[Use of soybean trypsin inhibitor for modification of gold surface of the sensor chips in surface plasmon resonance spectrometer]. / Zastosuvannia soievoho inhibitora Trypsynu dlia modyfikatsiï poverkhni zolota sensornykh chipiv spektrometra poverkhnevoho plazmonnoho rezonansu.

Kunitz soybean trypsin inhibitor (STI) and glutaraldehyde are used to create an interlayer for proteins immobilization on the gold surface of the sensor chips of surface plasmon resonance spectrometer. Human serum albumin (HSA), goat Ig G and bovine eye lens alpha-crystallin are immobilized via the proposed interlayer. We studied the effects of the duration of storage of the sensor chips before use and pre-treatment by "piranha" solution on the STI adsorption by the gold surface. The influence of STI surface concentration, as well as the effect of the duration of storage of STI-modified sensor chips on the HSA immobilization are investigated. The binding of specific antibodies to the immobilized proteins and non-specific binding to the modified surfaces are studied. HSA immobilization on the bare gold surface is compared to that on the surfaces, modified by different methods.

[Investigation of interaction of immunoglobulins and detection of viral antigens in cell homogenates by the surface plasmon resonance method]. / Doslidzhennia vzaiemodiï imunohlobuliniv ta vyiavlennia virusnykh antyheniv u klitynnykh homohenatakh metodom poverkhnevoho plazmonnoho rezonansu.

Immobilization of immunoglobulins on the unmodified gold surface and the gold surface, modified by thiocyanate and protein A Staphylococcus aureus and their subsequent interaction with complementary antibodies were studied using the surface plasmon resonance method. Viral antigens were detected by surface plasmon resonance method in the cell homogenate of green alga Bracteococcus minor, which was artificially infected by tobacco mosaic virus. It was shown, that the process of interaction between virus and antiviral serum obey the Langmure model.

[Comparative study of horseradish peroxidase immobilization on modification of a gold surface using a surface plasmon resonance method]. / Porivnial'ne doslidzhennia immobilizatsiï peroksydazy khrinu na modyfikovanii poverkhni zolota metodom poverkhnevoho plazmonnoho rezonansu.

Horseradish peroxidase is immobilized by a periodate method on the gold surfaces previously modified with 16-mercapto-hexadecanoic acid or with hydrogen disulfide and soybean trypsin inhibitor. The effect of gold surface modification conditions on the immobilization of the enzyme as well as on the properties of the immobilized glycoprotein are studied using surface plasmon resonance technique. Restoration of the ability to bind specific antibodies is demonstrated for the immobilized enzyme. The low level of non-specific antibody binding to the immobilized glycoprotein is also shown.