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1.
Plant Cell Environ ; 47(5): 1797-1812, 2024 May.
Article in English | MEDLINE | ID: mdl-38314665

ABSTRACT

As the most abundant form of methylation modification in messenger RNA (mRNA), the distribution of N6-methyladenosine (m6A) has been preliminarily revealed in herbaceous plants under salt stress, but its function and mechanism in woody plants were still unknown. Here, we showed that global m6A levels increased during poplar response to salt stress. Methylated RNA immunoprecipitation sequencing (MeRIP-seq) revealed that m6A significantly enriched in the coding sequence region and 3'-untranslated regions in poplar, by recognising the conserved motifs, AGACU, GGACA and UGUAG. A large number of differential m6A transcripts have been identified, and some have been proved involving in salt response and plant growth and development. Further combined analysis of MeRIP-seq and RNA-seq revealed that the m6A hypermethylated and enrich in the CDS region preferred to positively regulate expression abundance. Writer inhibitor, 3-deazaneplanocin A treatment increased the sensitivity of poplar to salt stress by reducing mRNA stability to regulate the expression of salt-responsive transcripts PagMYB48, PagGT2, PagNAC2, PagGPX8 and PagARF2. Furthermore, we verified that the methyltransferase PagFIP37 plays a positively role in the response of poplar to salt stress, overexpressed lines have stronger salt tolerance, while RNAi lines were more sensitive to salt, which relied on regulating mRNA stability in an m6A manner of salt-responsive transcripts PagMYB48, PagGT2, PagNAC2, PagGPX8 and PagARF2. Collectively, these results revealed the regulatory role of m6A methylation in poplar response to salt stress, and revealed the importance and mechanism of m6A methylation in the response of woody plants to salt stress for the first time.


Subject(s)
Adenosine/analogs & derivatives , Populus , RNA Methylation , Salt Stress/genetics , Methyltransferases/genetics , Populus/genetics , RNA, Messenger/genetics
2.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-248248

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the clinical effect of indirect cast post core by two different impression methods between silicone rubber impression and agar/alginate combination impression.</p><p><b>METHODS</b>389 massive destruction teeth after root canal therapy were randomly divided into two groups (group A and group B). The teeth of group A was treated by silicone rubber impression material for making indirect post core impression. The teeth of group B was treated by agar/alginate combination impression for making indirect post core impression. The effect of two different impression methods was evaluated.</p><p><b>RESULTS</b>The success rate of making indirect post core impression by silicone rubber impression method was higher than that of making impression by agar/alginate combination impression method. Significant difference was found in making indirect post core impression between premolars and molars by two different impression methods (P<0.05), while there was no significant difference in making indirect post core impression of anterior teeth by two different impression methods (P>0.05).</p><p><b>CONCLUSION</b>The clinical effect of making indirect post core impression by silicone rubber impression method is better than that of making impression by agar/alginate combination impression method.</p>


Subject(s)
Humans , Bicuspid , Crowns , Post and Core Technique , Root Canal Therapy
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