Impact of antimicrobial wipes compared with hypochlorite solution on environmental surface contamination in a health care setting: A double-crossover study.

OBJECTIVE: Antimicrobial wipes are increasingly used in health care settings. This study evaluates, in a clinical setting, the efficacy of sporicidal wipes versus a cloth soaked in a 1,000 ppm chlorine solution. INTERVENTION: A double-crossover study was performed on 2 different surgical and cardiovascular wards in a 1,000-bed teaching hospital over 29 weeks. The intervention period that consisted of surface decontamination with the preimpregnated wipe or cloth soaked in chlorine followed a 5-week baseline assessment of microbial bioburden on surfaces. Environmental samples from 11 surfaces were analyzed weekly for their microbial content. RESULTS: A total of 1,566 environmental samples and 1,591 ATP swabs were analyzed during the trial. Overall, there were significant differences in the recovery of total aerobic bacteria (P < .001), total anaerobic bacteria (P < .001), and ATP measurement (P < .001) between wards and between the different parts of the crossover study. Generally, the use of wipes produced the largest reduction in the total aerobic and anaerobic counts when compared with the baseline data or the use of 1,000 ppm chlorine. Collectively, the introduction of training plus daily wipe disinfection significantly reduced multidrug-resistant organisms recovered from surfaces. Reversion to using 1,000 ppm chlorine resulted in the number of sites positive for multidrug-resistant organisms rising again. CONCLUSIONS: This double-crossover study is the first controlled field trial comparison of using preimpregnated wipes versus cotton cloth dipped into a bucket of hypochlorite to decrease surface microbial bioburden. The results demonstrate the superiority of the preimpregnated wipes in significantly decreasing microbial bioburden from high-touch surfaces.

Pathogen transfer and high variability in pathogen removal by detergent wipes.

BACKGROUND: The rise in health care-associated infections has placed a greater emphasis on cleaning and disinfection practices. The majority of policies advocate using detergent-based products for routine cleaning, with detergent wipes increasingly being used; however, there is no information about their ability to remove and subsequently transfer pathogens in practice. METHODS: Seven detergent wipes were tested for their ability to remove and transfer Staphylococcus aureus, Acinetobacter baumannii, and Clostridium difficile spores using the 3-stage wipe protocol. RESULTS: The ability of the detergent wipes to remove S aureus, A baumannii, and C difficile spores from a stainless steel surface ranged from 1.50 log10 (range, 0.24-3.25), 3.51 log10 (range, 3.01-3.81), and 0.96 log10 (range, 0.26-1.44), respectively, following a 10-second wiping time. All wipes repeatedly transferred significant amounts of bacteria/spores over 3 consecutive surfaces, although the percentage of total microorganisms transferred from the wipes after wiping was low for a number of products. CONCLUSIONS: Detergent-based wipe products have 2 major drawbacks: their variability in removing microbial bioburden from inanimate surfaces and a propensity to transfer pathogens between surfaces. The use of additional complementary measures such as combined detergent/disinfectant-based products and/or antimicrobial surfaces need to be considered for appropriate infection control and prevention.

Efficacy of "sporicidal" wipes against Clostridium difficile.

BACKGROUND: Hospital-acquired infections associated with Clostridium difficile cause severe morbidity and mortality. The current control of C difficile endospores with liquid sporicides might have limited efficacy in the health care environment. Sporicidal wipes might offer additional control of surface bioburden and are now increasingly used, although there is little information about their efficacy against spores in practice. METHODS: Ten wipes were tested for sporicidal efficacy using a recently developed 3-stage protocol that measures the ability of the wipe to remove microbial bioburden from a surface, the potential for microbial transfer from the wipe to other surfaces, and the sporicidal activity of the wipe. Scanning electron microscopy was used to visualize the association of spores with the wipe fibers, and light scattering was used to measure the size of spore aggregates released from the wipes. RESULTS: The ability of the sporicidal wipes to remove C difficile spores from an inanimate surface ranged from 0.22 to 4.09 log(10) spores removed within 10 seconds. One wipe did not remove any spores. None of the wipes demonstrated high sporicidal activity (ie, >4 log(10) reduction) within 5 minutes of contact time, except for a control wipe soaked in 5,000-ppm sodium hypochlorite. Only one wipe demonstrated some sporicidal activity after 5 minutes, with a 1.50 and a 3.74 log(10) reduction in spore number of C difficile NCTC12727 and R20291 (ribotype 027), respectively. All but one wipe demonstrated that spores could be repeatedly transferred to other surfaces. Light-scattering data provided evidence that some wipes were able to break up spore aggregates, potentially releasing more spores onto the surface. Electron microscopy micrographs showed that spores might be loosely associated with some wipes, explaining the rapid release. CONCLUSION: Although the use of sporicidal wipes might offer additional control of microbial burden on surfaces, current efficacy tests might be inadequate to reflect the activity of these wipes in practice. This can lead to the use of wipes that might not be appropriate for applications in the health care environment. Tighter control of labeling and appropriate efficacy tests are needed before antimicrobial wipes are released to the market.