[Selection of thermophilic lactose-fermenting yeast strains].

The screening and selection of lactose-fermenting yeasts among 97 collection yeast strains belonging to different taxonomic groups has been conducted to obtain ethanol from whey. The strains (n=18) (1 strain of K. lactis. 16 strains of K. marxianus and 1 strain of C. kefyr) fermented lactose at 48 degrees C and 15 selected strains rapidly consumed lactose within 24-48 h of cultivation. The presence of 6% of ethanol in the medium resulted in a considerable growth inhibition (more than 80%) of the selected strains.

[Activity of oxidative enzymes in plant cells under conditions of experimental mycoplasmosis].

Changes in activity of oxidative enzymes was shown when infecting the callus culture of sugar beet cells by phytopathogenic mollicute Acholeplasma laidlawii var granulum 118. Maximum indices of the enzyme activity were fixed 3 hours after infecting with regard to control: it was 49% for peroxidase, 38% for catalase and 45% for polyfenoloxidase. The decrease of enzymatic activity to 22%, 12% and 19%, respectively, was registered 5 hours after infecting, and after that all these indices became stabilized. Changes in the activity of components of antioxidative protection were probably connected with induction of plants' protective mechanisms in response to penetration of pathogenic mollicutes.

[Comparative study of the monosaccharide glycocalyx composition of Bacillus--Lactobacillus--Streptococcus bacteria group by biochemical methods and electron microscopy]. / Porivnial'ne vyvchennia skladu monotsukriv hlikokaliksu bakterii hrupy Bacillus--Lactobacillus--Streptococcus biokhimichnymy ta elektronnomikroskopichnym metodamy.

Results of comparison of the data on monosaccharide composition of glycocalix in group Bacillus--Lactobacillus--Streptococcus bacteria using biochemical and electronmicroscopic methods prove that the composition and quantity of monosaccharides varied depending on the genus and peculiarities of microorganisms strains (biological activity in respect of people and warm-blooded animals). At the same time, representatives of this group of microorganisms in the glycocalix had common monosaccharides: glucosamine, sialic acids, galactosamine, galactose, glucose, mannose, fucose, that is the phenotypical display of genetic relations.

[Analysis of monosaccharides of bacterial glycocalix of Bacillus-Lactobacillus-Streptococcus group by gas-chromatography]. / Hazokhromatohrafichnyi analiz monosakharidnoho skladu hlikokaliksu bakterii hrupy Bacillus-Lactobacillus-Streptococcus.

The glycocalix of some bacteria of the Bacillus--Lactobacillus--Streptococcus (Bacillus subtilis 3, B. subtilis 668, B. licheniformis 31, Lactobacillus plantarum 11/16, Enterococcus faecium K-50) group were isolated and composition of neutral monosaccharides was investigated. Such sugars as rhamnose, ribose, fucose, arabinose, xylose, mannose, galactose, and glucose were found in the investigated representatives of genus Bacillus--Lactobacillus--Streptococcus. Presence of significant quantity of glucose and galactose did not depend on cultivation conditions. This fact testifies to its conservatism and a possibility to use it as phylogenetic marker.

[Monosaccharide composition of some Mollicutes]. / Monosakharydnyi sklad poverkhnevykh struktur deiakykh Molikutiv.

When studying a small group of mollicutes which interact in different way with the host organism (Mycoplasma fermentans PG18--is considered a commensal of the human urogenital tract, and sometimes manifests as AIDS cofactor, Acholeplasma laidlawii PG8 is saprophytic but it is connected with various pathologies in human organism, A. laidlawii var. granulum 118--agent of the yellow of cereals--palegreen dwarfness) the author has found the carbohydrate composition of their cell surface. Glycocalix of all three representatives proved to be rather various. Biochemical investigation methods were used to establish the presence of galactosamine, glucosamine, minor quantity of rhamnose, ribose, arabinose, xylose, mannose as well as considerable share of fucose, galactose, glucose. Qualitative and quantitative content of monosaccharide composition of the glycocalix of certain species of mollicutes which have different ecological niches is studied in the work and the role of some sugars in performing certain important functions is discussed.

[The effect of antibiotic preparations and their combinations with probiotics on the intestinal microflora of mice]. / Vplyv antybiotychnykh preparativ ta ïkh kombinatsii z probiotykamy na mikrofloru kyshechnyka myshei.

Antibiotic drugs (biseptol, polymyxin, canamycin) as well as their combinations with probiotics biosporin and subalin have been studied for their effect on mice intestine microflora. It has been established that under peroral administration of the above drugs dysbacteriosis of the 2-3rd degrees arises in animals; under their combined use with probiotics one can prevent the development of dysbacteriosis to considerable extent. It has been shown that dysbacteriosis of the 2-3rd degrees can be liquidated for 5-7 days, using probiotics biosporin and subalin. No self-recovery of normal intestinal microbiocenosis is observed in this period. Inconsiderable variations in the composition of normoflora under combined use of antibiotics with probiotics are renewed on the 3-5th day after stopping the drugs intake.