ABSTRACT
The association process of chlorpromazine with bovine serum albumin by both onefactoral passive and multifactoral active experiments were investigated in model systems. The dependence of absolute concentration of the free fraction of chlorpromazine on the total concentration of chlorpromazine is described by the increasing function, but the dependence of the relative concentration (free/total) on this factor is influenced by external characteristics. The concentrations of chlorpromazine, bovine serum albumin and Tritons' mixture, as principal factors of a multifactoral experiment, influence the absolute and relative values of the free fraction of chlorpromazine non-additively, ie the interaction of factors is significant.
Subject(s)
Antipsychotic Agents/metabolism , Chlorpromazine/metabolism , Serum Albumin, Bovine/metabolism , Animals , Cattle , Chromatography, High Pressure Liquid , Protein Binding , Spectrophotometry, UltravioletABSTRACT
A method for the determination of the free fraction of antiepileptic drugs in plasma and saliva was developed. The separation of free and protein-bound fractions was obtained by cloud-point extraction under optimum conditions of pH, surfactant type, and concentration. It is shown that the free fraction of carbamazepine and of phenobarbital in plasma coincides with the drug concentration in saliva. The dependence of the free fraction in plasma on the administered dose was studied. The influence of the simultaneous administration of the two drugs on their free concentrations was revealed: In the presence of carbamazepine the free fraction of phenobarbital is decreased markedly whereas phenobarbital does not influence the behavior of carbamazepine.
