ABSTRACT
Taking into account clinical significance of determination of medium-molecular peptides' concentration in various surgical diseases and limitation of blood sampling in children, modification of the method for determination of medium-molecular peptides in blood serum is proposed, which requires 0.1 ml of serum and is based on deproteinization of cooled ethanol followed by spectrophotometry of peptide solution at 210 nm. Comparison study of the results of examination by this method and routine one was carried out. Specificity and advantages of the suggested modification are discussed.
Subject(s)
General Surgery , Pediatrics , Peptides/blood , Spectrophotometry/methods , Adolescent , Bacterial Infections/blood , Bacterial Infections/surgery , Biomarkers/blood , Child , Child, Preschool , Humans , Severity of Illness Index , Wounds and Injuries/blood , Wounds and Injuries/surgeryABSTRACT
The paper summarizes experimental and clinical findings of neutrophilic responsiveness and the expediency of its measurements by using functional loading tests and the law of initial values. A concept of neutrophilic responsiveness phases at the cellular and tissue levels, the typology of responses to various factors, and the need to individualize the objects of pathophysiological investigations are discussed.
Subject(s)
Granulocytes/pathology , Liver Diseases/pathology , Animals , Clinical Trials as Topic , Evaluation Studies as Topic , HumansABSTRACT
1, 3, 5, 7 days after the 24-hr immobilisation stress, the concentration of nuclear-segmental neutrophils and basic proteins were determined in peripheral blood of white rats. 4 patterns of the neutrophils dynamics were revealed. Individual character of decationisation and granulocytopoiesis potentiation was suggested by the data obtained.
Subject(s)
Granulocytes/physiology , Stress, Psychological/blood , Animals , Blood Proteins/analysis , Bone Marrow/pathology , Female , Leukocyte Count , Male , Neutrophils/physiology , Rats , Restraint, Physical , Time FactorsABSTRACT
Changes in the excretion of tissue-specific inhibitor and myelopoiesis stimulator (chalone and antichalone) by the mature neutrophils in respect to intracellular concentration interactions and the presence of products of autologous cells splitting, were studied on the models in vivo and in vitro.
Subject(s)
Granulocytes/metabolism , Growth Inhibitors/metabolism , Hematopoiesis/physiology , Animals , Bone Marrow/drug effects , Bone Marrow Cells , Cell Communication/physiology , Cells, Cultured/metabolism , Growth Inhibitors/analysis , Growth Inhibitors/pharmacology , Mice , Neutrophils/metabolism , Rats , SwineABSTRACT
The experiments on rats have shown that immobilization stress induces a prolonged increase of the activity in the blood serum of tissue-specific granulocytopoiesis stimulator--granulocytic antichalone.
Subject(s)
Granulocytes/physiology , Growth Inhibitors/physiology , Stress, Physiological/physiopathology , Animals , Growth Inhibitors/antagonists & inhibitors , Male , RatsABSTRACT
Investigation of the blood genetic markers ADB, Rh, Hp and Tr in 186 patients with chronic infectious inflammatory diseases of the lung revealed a significant increase in the blood group B(III), Rh- and phenotype Hp 2-2. It is proposed to consider the association of the blood group B(III), Rh- and Hp 2-2. as an unfavourable prognostic sign of infectious inflammatory diseases of the lung.
Subject(s)
Genetic Markers , Lung Diseases/genetics , ABO Blood-Group System , Adult , Aged , Asthma/blood , Asthma/genetics , Bronchitis/blood , Bronchitis/genetics , Female , Haptoglobins/genetics , Humans , Lung Diseases/blood , Male , Middle Aged , Phenotype , Pneumonia/blood , Pneumonia/genetics , Prognosis , Rh-Hr Blood-Group SystemABSTRACT
Studies of haptoglobin phenotypes (Hp) by disk electrophoresis in 173 patients with various, infectious inflammatory pulmonary diseases have revealed a growing incidence rate of Hp2-2. This has prompted a conclusion that Hp2-2 may be regarded as a serum genetic marker indicating a high risk of an inflammatory pulmonary disease.
Subject(s)
Haptoglobins/genetics , Lung Diseases/genetics , Adolescent , Adult , Aged , Genetic Markers , Humans , Middle Aged , PhenotypeSubject(s)
Granulocytes/physiology , Growth Inhibitors/blood , Homeostasis , Inflammation/blood , Animals , Bone Marrow/drug effects , Bone Marrow Cells , Cell Division/drug effects , Dose-Response Relationship, Drug , Growth Inhibitors/antagonists & inhibitors , Growth Inhibitors/isolation & purification , Growth Inhibitors/pharmacology , Hematopoiesis/drug effects , Homeostasis/drug effects , Inflammation/chemically induced , Mice , Neutrophils/cytology , Neutrophils/drug effects , Rats , Starch , Time FactorsABSTRACT
The normal physiological balance between granylocytic chalone and antichalone is shown to be significantly upset where an inflammatory focus is present, with segmented neutrophils activated by cell breakdown products producing, for the most part, a stimulator of cell proliferation. It is also shown that the production of tissue-specific regulators in inflammation depends on the functional state of the entire mature neutrophil pool. The mechanisms of intrasystemic granulocytopoiesis regulation have been unraveled.
Subject(s)
Granulocytes/physiology , Growth Inhibitors/physiology , Hematopoiesis , Animals , Cell Division , Growth Inhibitors/antagonists & inhibitors , Homeostasis , Inflammation/physiopathology , Mice , Neutrophils/physiology , Rats , Staphylococcal Infections/physiopathologySubject(s)
Bronchi/metabolism , Bronchitis/metabolism , Electrophoresis, Disc/methods , Proteins/analysis , Adult , Female , Humans , Male , Middle AgedABSTRACT
Experiments on 107 rabbits and 76 white rats with the use of electrophysiological, biochemical and electron microscopy research methods have shown that nonachlazine-induced improvement of heart contractility in microfocal myocardial infarction occurs as a result of the shortening of the systolic and lengthening of the diastolic phases of the heart cycle. Nonachlazine improves the processes of oxidative phosphorylation in heart mitochondria in myocardial infarction, which is accompanied by inhibition of the activity of succinate dehydrogenase and transition of oxidation from succinate to NAD-dependent substrates.
