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1.
Vestn Khir Im I I Grek ; 161(4): 47-51, 2002.
Article in Russian | MEDLINE | ID: mdl-12577553

ABSTRACT

Cryopreservation is the method of choice in solution of the problem of the done in good time reservation of the components of blood and bone marrow for autotransfusion in patients referred to the contingent of particular risk. Freezing of autologous hemocomponents increases the infection safety and efficiency of therapy with hemocomponents. The wide introduction of using autologous hemocomponents into the everyday surgical practice and in the arsenal of means and methods of the medicine of extreme situations is an indispensable condition for increasing the effects of curative and medico-evacuational measures.


Subject(s)
Blood Banks , Blood Component Transfusion , Blood Preservation , Blood Transfusion, Autologous , Bone Marrow , Cryopreservation , Organ Preservation , Emergencies , Humans , Risk Factors , Time Factors
2.
Med Tekh ; (1): 18-9, 2001.
Article in Russian | MEDLINE | ID: mdl-11244848

ABSTRACT

A new device for preparing washed red cell components was proposed by the Sintez Co., Kurgan. The disposable containers were evaluated at the Center of Blood and Tissues, Military Medical Academy. The new system was found to be more effective, time-saving, cost-effective and decreased the risk of infectious complications as compared to the traditional methods for washing red cell components. Thus, the new device can be recommended for wide use in transfusion medicine.


Subject(s)
Blood Preservation , Cryopreservation , Erythrocyte Transfusion , Erythrocytes , Cost-Benefit Analysis , Costs and Cost Analysis , Cytological Techniques , Erythrocyte Transfusion/economics , Erythrocyte Transfusion/instrumentation , Erythrocyte Transfusion/methods , Humans
3.
Med Tekh ; (1): 45-7, 2000.
Article in Russian | MEDLINE | ID: mdl-10740789

ABSTRACT

The first Russian solution Krimolit designed for cryopreservation of platelet concentrates down -196 degrees C was clinically tested. The therapeutical efficacy of the cryopreserved platelets was evaluated on the basis of clinical and laboratory monitoring of transfusions in 20 cancer hematological patients. They were found to have the same therapeutical action as fresh cells. The introduction of cryopreserved platelets into clinical practice allows one to form stores and to transfuse autological cells. Krimolit is recommended for clinical application.


Subject(s)
Blood Platelets , Blood Preservation , Cryopreservation , Platelet Transfusion , Blood Preservation/methods , Blood Transfusion, Autologous , Cryopreservation/methods , Evaluation Studies as Topic , Humans , Solutions , Time Factors
7.
Med Tekh ; (3): 34-6, 1998.
Article in Russian | MEDLINE | ID: mdl-9720017

ABSTRACT

A new system for manual-2 plasmocytapheresis from a medical polymer plant ready for use (Medpolymer, St. Petersburg) was evaluated in 15 donors. It makes manual-2 plasmapheresis more efficient, time-saving, and cost-effective. Its also decreases the contamination of blood components and the risk of air embolic complications. Thus, the new system may be recommended for use in transfusion medicine.


Subject(s)
Plasmapheresis/instrumentation , Adolescent , Adult , Equipment Design , Female , Humans , Male , Plasmapheresis/methods , Russia
8.
Klin Lab Diagn ; (11): 15-7, 1998 Nov.
Article in Russian | MEDLINE | ID: mdl-10205652

ABSTRACT

Laboratory testing of donor blood is the key point in prevention of hemotransmissive syphilis. The probability of using diagnostic EIA kit RecombiBest antipallidum-strip (AO Vektor-Best, Novosibirsk) is assessed. Ninety-two donor sera were tested. For assessing the sensitivity of the test system, 11 sera of syphilis patients were titrated by double dilutions 1:10 to 1:1280 for each strip. In parallel with this, antibodies to T. pallidum were assessed in the precipitation microtest. EIA proved to be not only highly specific, but more sensitive than precipitation microtest. Antibodies to T. pallidum are detected by EIA in serum dilutions at least two times higher than the minimal titers in the precipitation test. Thus, the above diagnostic kit is recommended for screening of donors of blood and its components.


Subject(s)
Blood Donors , Syphilis Serodiagnosis/methods , Antibodies, Bacterial/blood , Biomarkers/blood , Humans , Immunoenzyme Techniques/instrumentation , Immunoenzyme Techniques/statistics & numerical data , Reagent Kits, Diagnostic/statistics & numerical data , Sensitivity and Specificity , Syphilis Serodiagnosis/instrumentation , Syphilis Serodiagnosis/statistics & numerical data , Treponema pallidum/immunology
9.
Vopr Virusol ; 42(6): 283-4, 1997.
Article in Russian | MEDLINE | ID: mdl-9499244

ABSTRACT

The Avicenna test system (St. Petersburg) for detecting antibodies to hepatitis C is assessed. Two-hundred and ten sera with increased SGPT activity and 73 samples seropositive in screening by the Aquapast test system (St. Petersburg) were examined. Avicenna system with 3 synthetic peptides as the test objects is characterized by a higher sensitivity and specificity and is recommended for practical use at blood service institutions.


Subject(s)
Blood Donors , Hepatitis C Antibodies/blood , Adolescent , Adult , Alanine Transaminase/blood , Humans , Immunoenzyme Techniques , Male , Sensitivity and Specificity
10.
Voen Med Zh ; 318(10): 12-6, 79, 1997 Oct.
Article in Russian | MEDLINE | ID: mdl-9446280

ABSTRACT

In the work the factors, rendering essential influence on organization of donorship in modern army conditions are systematized. The organization of donorship should be connected to functioning of territorial system of medical maintenance of the Armed Forces of RF. It assumes presence of four organizational levels: central, district, zone and garrison. Perfecting the system of organization of donorship, we should be guided by the following conducting principles: maximum maintenance of safety of donorship; wide introduction of a method of plasmacytopheresis in work of establishments and subdivisions of blood supply service; organization of autodonorship and quota of the donors of an emergency reserve in each military-medical establishment; reliable maintenance of revealing of markers of hemotransmissible infections at inspection of the donors.


Subject(s)
Blood Donors , Military Personnel , Adaptation, Physiological , Blood Donors/psychology , Humans , Military Medicine/organization & administration , Military Personnel/psychology , Naval Medicine/organization & administration , Russia , Safety
11.
Vopr Virusol ; 41(4): 185-7, 1996.
Article in Russian | MEDLINE | ID: mdl-8999677

ABSTRACT

An original methodological approach to the diagnosis of Epstein-Barr virus (EBV) infection is proposed, consisting in the detection of viral DNA in peripheral blood lymphocytes using in situ DNA hybridization. EBV DNA was found in 6 (50%) patients with lymphomas and in 1 (33%) with infective endocarditis out of 26 examined recipients of blood components. No EBV DNA was found in peripheral blood lymphocytes of 14 regular donors. Normalization of the immune status of a patient and use of blood components containing no EBV is the key factor in the prevention of EBV infection. Biohit test system for in situ hybridization of EBV DNA may be used for diagnostic monitoring of EBV infection and screening of blood and tissue donors.


Subject(s)
DNA, Viral/analysis , Herpesviridae Infections/diagnosis , Herpesvirus 4, Human/isolation & purification , Tumor Virus Infections/diagnosis , Herpesviridae Infections/blood , Herpesvirus 4, Human/genetics , Humans , In Situ Hybridization , Leukocytes, Mononuclear/virology , Sensitivity and Specificity , Tumor Virus Infections/blood
12.
Klin Lab Diagn ; (3): 29-30, 1996.
Article in Russian | MEDLINE | ID: mdl-8925209

ABSTRACT

A new two-staged method for identifying the rhesus appurtenance of the blood is proposed. During the first stage anti-D-super (IgM monoclonal anti-D-antibodies) are used, during the second stage rhesus appurtenance is identified in a tube using standard universal reagent for determining rhesus factor RhO(D) or it is done using gelatin test with Anti-D (IgG monoclonal anti-D-antibodies). A negative result at stage 1 and positive at stage 2 is a sign of partial antigen D, most probably of the VIth category. Subjects with partial D are qualified as rhesus-positive donors and rhesus-negative recipients.


Subject(s)
Blood Donors/classification , Rh-Hr Blood-Group System/immunology , Antibodies, Monoclonal , Erythroblastosis, Fetal/diagnosis , Female , Gelatin , Humans , Immunoglobulin G/immunology , Indicators and Reagents , Infant, Newborn , Male , Pregnancy , Rh-Hr Blood-Group System/classification , Rh-Hr Blood-Group System/genetics
13.
Voen Med Zh ; 317(4): 25-6, 79, 1996 Apr.
Article in Russian | MEDLINE | ID: mdl-8754054

ABSTRACT

The article describes the new two-stage method of determining of Rh-factor belonging. At the first stage tsoliclon "Anti-D-super" (monoclonic anti-D-antibody class IgM) was used. At the second stage Rh-factor belonging is determined in test-tube, using standard universal reagent for definition Rh-factor RhO (D). Combination of negative results at the first stage with positive ones at the second stage is a sign of partial antigen D, most probably category VI. The persons with partial D are qualified as Rh-positive donors and Rh-negative recipients.


Subject(s)
Blood Grouping and Crossmatching/methods , Rh-Hr Blood-Group System/blood , Antibodies, Monoclonal , Erythrocytes/immunology , Female , Humans , Immunoglobulin M , Indicators and Reagents , Rh-Hr Blood-Group System/classification
14.
Vopr Virusol ; 41(2): 61-3, 1996.
Article in Russian | MEDLINE | ID: mdl-8686274

ABSTRACT

The sensitivities and specificities of enzyme immunoassay (EIA) kits for the detection of antibodies to hepatitis C virus (HCV) were compared in donor blood screening. A total of 3308 donors were examined using Akvapast immunodiagnosticum (St. Petersburg), lot 12. Anti-HCV antibodies were detected in 43 (1.3%) subjects. Blood sera of these donors were additionally investigated using other lots of Akvapast, Hepascan (Bioservice, Moscow), Blot-0.5 (Bioservice, Moscow), and immunoComb (France). The studies demonstrated that none of the agents used can be acknowledged as ideally sensitive and specific for the detection of anti-HCV antibodies. Maximally sensitive diagnostic kits should be preferred for the screening of donors of blood components and for other mass surveys. Highly-specific test systems are advisable for profound examinations and diagnostic monitoring. The creation of a system of reference and expert diagnosis of hepatitis C remains a pressing task.


Subject(s)
Hepatitis C Antibodies/blood , Reagent Kits, Diagnostic , Adolescent , Adult , Blood Donors , Evaluation Studies as Topic , Humans , Male , Sensitivity and Specificity
17.
Voen Med Zh ; (6): 44-5, 80, 1994 Jun.
Article in Russian | MEDLINE | ID: mdl-7941454

ABSTRACT

The article contains data on viral hepatitis C, its etiological factor, epidemiology, methods of diagnosis and prophylaxis. It's high time to conduct 100% screening control of donors for viral hepatitis C antibody, using national test system of fermental analysis. The authors worked out the method of "dry drop" to study the serum for hepatitis C virus antibody.


Subject(s)
Hepatitis C/prevention & control , Military Personnel , Transfusion Reaction , Blood Donors , Hepacivirus/immunology , Hepatitis Antibodies/blood , Hepatitis C/diagnosis , Hepatitis C/transmission , Humans , Immunoenzyme Techniques , Russia
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