[Pharmacological neuroprotection against experimental acute cerebral ischemia of neurons of sensomotor area of frontal cortex and hippocampus in rats].

Emoxypine, thiotriazoline, and NN-103 (a 4-hydrazinoquinasoline derivative) exhibit a pronounced neuroprotective action during acute cerebral stroke. Emoxypine and thiotriazoline are more effective in inhibiting death of neurons in the sensomotor area of the frontal cortex, while NN-103 is more active in the hippocampus. The ischemic death of neurons in the sensomotors area of the frontal cortex with equal probability takes place by karyopyknosis or cytolysis, while in the hippocampus, mainly by cytolysis. All preparations (except for piracetam) with antioxidants properties are powerful membranoprotective agents, which is confirmed by a considerable decrease in the rate of cytolysis both in the cortex and in the hippocampus. The use of piracetam in the case of acute cerebral stroke increases the rate of neuronal death in various areas of the cortex, up to complete destruction of nerve tissues with the formation of cysts (in hippocampus). The tested preparations exhibit different mechanism of neuroprotection: emoxypine and NN-103 (in the sensomotor area of the frontal cortex and hippocampus) and thiotriazoline (in the hippocampus) produce the neuroprotection action on the background of increased activity of transmissions and transcription processes, while thiotriazoline (in the sensomotor area of the frontal cortex) offers neuroprotection on the background of inhibited transcription and transmission activity.

[The role of 5 alpha-dihydrotestosterone in regulating the sexual maturation of male rabbits]. / Rol' 5 al'fa-digidrotestosterona v reguliatsii polovogo sozrevaniia samtsov krolikov.

The data obtained suggest a greater sensitivity of the prepubertal rabbits' hypophysis for small doses of 5 alpha-dihydrotestosterone exerting an inhibitory effect. The data emphasizes the significance of the agent for regulation of sexual maturation.

[Prolonged antibacterial action of polymer coated suture materials]. / Prolongirovannoe antibakterial'noe deistvie shovnykh materialov s polimernym pokrytiem.

The antimicrobial activity of capromed, a surgical polymer-coated sutural material containing dioxidine, quinoxidine or gentamicin was studied in vitro and in vivo. Capromed was shown to be active against the hospital strains of S. aureus, E. coli, Proteus spp., Klebsiella spp. and P. aeruginosa. The antimicrobial activity of ADH and AG threads was preserved for 3 to 4 days after implantation. The DH-2 and G-2 threads preserved their activity for 6 to 7 days. It was concluded that the duration of thread antimicrobial activity depended on the properties of the polymer coating the thread. Capromed was applied to 280 operation wounds in 275 patients. There was no wound suppuration in the group of patients after pure operations (n = 62). In the group of patients after conditionally pure operations (n = 130) suppuration was observed in 2 patients (1.3 per cent). In patients with contaminated wounds (n = 88) suppuration in 5 of them (5.7 per cent) was recorded. The total number of the purulent complications after using capromed in surgical operations amounted to 2.5 per cent. In the control group purulent complications were stated in 8.2 per cent of the cases.

[The dynamics of the wound microflora in the victims of the earthquake in Armenia]. / Dinamika mikroflory ran postradavshikh pri zemletriasenii v Armenii.

The data on the composition of wound microflora, as well as changes in the contamination index over the course of treatment of patients with the syndrome of prolonged compression, who were brought to the institute after the earthquake in Armenia, are presented. The authors characterize the structure of the causative agents of wound infection in the patients treated at the traumatological department of the institute over several years. The epidemiological situation in the hospital in the period when the earthquake victims were treated there is analyzed.

[The microfloral wound dynamics of the victims in the railroad disaster in Bashkiria]. / Dinamika mikroflory ran postradavshikh pri katastrofe na zheleznoi doroge v Bashkirii.

The work deals with the microbial composition of burn wounds in the victims of the railroad catastrophe in Bashkiria during their stay in a hospital. The comparison of the microbial spectrum in the wounds of the burn victims of the catastrophe with the results of the monitoring of the causative agents of burn infections in patients treated at the department of thermal lesions over a period of 3 years is presented. The medicinal sensitivity of wound microflora at all stages of the hospital treatment of the patients was studied and compared with the medicinal sensitivity of microorganisms isolated from the wounds of burn patients in 1988.

[The role of prolactin in the pathogenesis of male sterility]. / Rol' prolaktina v patogeneze muzhskogo besplodiia.

To study the causal relationship between the hormonal status and spermatographic data in sterile males, the authors investigated the correlations between the plasma levels of testosterone, estradiol, prolactin and gonadotrophin and certain parameters of spermograms and computed the correlative relationship of those values. The findings demonstrated the increased levels of prolactin (249.09 +/- 39.6 mu/U/ml), significantly exceeding the normal (132.6 +/- 32.4 mu/U/ml), a tendency towards a decrease in the levels of FSH and testosterone (up to 7.01 +/- 0.70 nmol/l versus 12.57 +/- 0.39 nmol/l in health) and manifest elevation of estradiol levels (up to 634.71 +/- 29.16 nmol/l versus 205.02 +/- 18.60 nmol/l), as well as its ratio to the testosterone levels. The number of spermatozoa in 1 ml was found decreased up to 29.6 +/- 4.10 million, the volume of ejaculate was reduced to 2.7 +/- 0.41 ml, while the percentage of immobile spermatozoa rose to 40.00 +/- 4.76. Despite of some pathological changes evidenced by spermograms, there was the only parameter--the volume of ejaculate--that was directly dependent on the levels of prolactin, their correlation was reversed. Decreased numbers of spermatozoa and their motility were not related to hyperprolactinemia. Moreover, there was a positive correlation between the levels of prolactin and the motility of spermatozoa in sterile patients. Therefore, decreased number and motility of spermatozoa turned to be dependent not on prolactin but sex hormone levels. However, ejaculation was found unfeasible in male with high levels of hyperprolactinemia.

Structural organization of the carbohydrate moiety of human transcortin as determined by methylation analysis of the whole glycoprotein.

Methylation analysis of human transcortin showed that this glycoprotein contains N-glycosidically linked oligosaccharide chains of N-acetyllactosamine type, most of the chains being biantennary and others tri- and/or tetra-antennary. The carbohydrate chains of transcortin are also heterogeneous with respect to the content of fucose and the position of the glycosidic linkages.

Methylation analysis in glycoprotein chemistry. General procedure for quantification of the products of solvolysis of permethylated glycopeptides and glycoproteins.

A technique for the gas chromatographic analysis of the products of solvolysis of permethylated glycopeptides and glycoproteins has been developed. It involves methanolysis of a permethylated compound, quantitative transformation of methyl ethers of methyl glycosides into the corresponding O-trimethylsilyl(TMS)-O-methylalditols [2-deoxy-2-(N-methyl)acetamido-O-TMS-O-methylalditols in the case of hexosamine derivatives] and gas chromatographic quantification using a single column packed with 0.4% OV-225 on surface-modified Chromosorb.