Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 7 de 7
Filter
Add more filters










Database
Language
Publication year range
1.
Curr Opin Microbiol ; 25: 67-72, 2015 Jun.
Article in English | MEDLINE | ID: mdl-26025019

ABSTRACT

Bet-hedging is an evolutionary theory that describes how risk spreading can increase fitness of a genotype in an unpredictably changing environment. To achieve risk spreading, maladapted phenotypes develop within isogenic populations that may be fit for a future environment. In recent years, various observations of microbial phenotypic heterogeneity have been denoted as bet-hedging strategies, sometimes without sufficient evidence to support this claim. Here, we discuss selected examples of microbial phenotypic heterogeneity that so far do seem consistent with the evolutionary theory concept of bet-hedging.


Subject(s)
Bacterial Physiological Phenomena , Biological Evolution , Gene-Environment Interaction , Genetic Fitness , Genotype , Models, Biological , Phenotype
2.
PLoS One ; 10(4): e0123239, 2015.
Article in English | MEDLINE | ID: mdl-25886351

ABSTRACT

Protein-protein interactions can be studied in vitro, e.g. with bacterial or yeast two-hybrid systems or surface plasmon resonance. In contrast to in vitro techniques, in vivo studies of protein-protein interactions allow examination of spatial and temporal behavior of such interactions in their native environment. One approach to study protein-protein interactions in vivo is via Förster Resonance Energy Transfer (FRET). Here, FRET efficiency of selected FRET-pairs was studied at the single cell level using sensitized emission and Frequency Domain-Fluorescence Lifetime Imaging Microscopy (FD-FLIM). For FRET-FLIM, a prototype Modulated Electron-Multiplied FLIM system was used, which is, to the best of our knowledge, the first account of Frequency Domain FLIM to analyze FRET in single bacterial cells. To perform FRET-FLIM, we first determined and benchmarked the best fluorescent protein-pair for FRET in Bacillus subtilis using a novel BglBrick-compatible integration vector. We show that GFP-tagRFP is an excellent donor-acceptor pair for B. subtilis in vivo FRET studies. As a proof of concept, selected donor and acceptor fluorescent proteins were fused using a linker that contained a tobacco etch virus (TEV)-protease recognition sequence. Induction of TEV-protease results in loss of FRET efficiency and increase in fluorescence lifetime. The loss of FRET efficiency after TEV induction can be followed in time in single cells via time-lapse microscopy. This work will facilitate future studies of in vivo dynamics of protein complexes in single B. subtilis cells.


Subject(s)
Bacillus subtilis/metabolism , Fluorescence Resonance Energy Transfer/methods , Green Fluorescent Proteins/metabolism , Humans , Microscopy, Fluorescence , Molecular Sequence Data
3.
ACS Synth Biol ; 3(12): 999-1002, 2014 Dec 19.
Article in English | MEDLINE | ID: mdl-25524109

ABSTRACT

Here, we developed a cell-based biosensor that can assess meat freshness using the Gram-positive model bacterium Bacillus subtilis as a chassis. Using transcriptome analysis, we identified promoters that are specifically activated by volatiles released from spoiled meat. The most strongly activated promoter was PsboA, which drives expression of the genes required for the bacteriocin subtilosin. Next, we created a novel BioBrick compatible integration plasmid for B. subtilis and cloned PsboA as a BioBrick in front of the gene encoding the chromoprotein amilGFP inside this vector. We show that the newly identified promoter could efficiently drive fluorescent protein production in B. subtilis in response to spoiled meat and thus can be used as a biosensor to detect meat spoilage.


Subject(s)
Bacillus subtilis/genetics , Bacillus subtilis/metabolism , Biosensing Techniques/methods , Genetic Engineering/methods , Meat/analysis , Bacillus subtilis/chemistry , Bacteriocins/genetics , Bacteriocins/metabolism , Fluorescent Dyes , Gene Expression Profiling , Peptides, Cyclic/genetics , Peptides, Cyclic/metabolism , Plasmids , Synthetic Biology , Volatile Organic Compounds
4.
ISME J ; 8(1): 77-87, 2014 Jan.
Article in English | MEDLINE | ID: mdl-23924781

ABSTRACT

Bacillus subtilis sporulation is a last-resort phenotypical adaptation in response to starvation. The regulatory network underlying this developmental pathway has been studied extensively. However, how sporulation initiation is concerted in relation to the environmental nutrient availability is poorly understood. In a fed-batch fermentation set-up, in which sporulation of ultraviolet (UV)-mutagenized B. subtilis is repeatedly triggered by periods of starvation, fitter strains with mutated tagE evolved. These mutants display altered timing of phenotypical differentiation. The substrate for the wall teichoic acid (WTA)-modifying enzyme TagE, UDP-glucose, has recently been shown to be an intracellular proxy for nutrient availability, and influences the timing of cell division. Here we suggest that UDP-glucose also influences timing of cellular differentiation.


Subject(s)
Bacillus subtilis/cytology , Bacillus subtilis/physiology , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Cell Division/physiology , Bacillus subtilis/enzymology , Bacillus subtilis/genetics , Bacillus subtilis/metabolism , Cell Division/genetics , Environment , Genome, Bacterial/genetics , Glucose/metabolism , Mutation/genetics , Spores, Bacterial/genetics , Spores, Bacterial/metabolism
5.
J Biol Chem ; 283(37): 25186-25199, 2008 Sep 12.
Article in English | MEDLINE | ID: mdl-18606812

ABSTRACT

Streptomycetes are exploited for production of a wide range of secondary metabolites, and there is much interest in enhancing the level of production of these metabolites. Secondary metabolites are synthesized in dedicated biosynthetic routes, but precursors and co-factors are derived from the primary metabolism. High level production of antibiotics in streptomycetes therefore requires engineering of the primary metabolism. Here we demonstrate this by targeting a key enzyme in glycolysis, phosphofructokinase, leading to improved antibiotic production in Streptomyces coelicolor A3(2). Deletion of pfkA2 (SCO5426), one of three annotated pfkA homologues in S. coelicolor A3(2), resulted in a higher production of the pigmented antibiotics actinorhodin and undecylprodigiosin. The pfkA2 deletion strain had an increased carbon flux through the pentose phosphate pathway, as measured by (13)C metabolic flux analysis, establishing the ATP-dependent PfkA2 as a key player in determining the carbon flux distribution. The increased pentose phosphate pathway flux appeared largely because of accumulation of glucose 6-phosphate and fructose 6-phosphate, as experimentally observed in the mutant strain. Through genome-scale metabolic model simulations, we predicted that decreased phosphofructokinase activity leads to an increase in pentose phosphate pathway flux and in flux to pigmented antibiotics and pyruvate. Integrated analysis of gene expression data using a genome-scale metabolic model further revealed transcriptional changes in genes encoding redox co-factor-dependent enzymes as well as those encoding pentose phosphate pathway enzymes and enzymes involved in storage carbohydrate biosynthesis.


Subject(s)
Gene Expression Regulation, Bacterial , Phosphofructokinase-1/genetics , Phosphofructokinase-1/physiology , Streptomyces coelicolor/metabolism , Amino Acid Sequence , Anthraquinones/chemistry , Base Sequence , Carbon/chemistry , Fructosephosphates/chemistry , Gene Deletion , Genome , Glucose-6-Phosphate/chemistry , Models, Biological , Molecular Sequence Data , Oxidation-Reduction , Prodigiosin/analogs & derivatives , Prodigiosin/chemistry
6.
J Microbiol Methods ; 58(1): 139-42, 2004 Jul.
Article in English | MEDLINE | ID: mdl-15177913

ABSTRACT

Streptomycetes are complex soil bacteria capable of producing aerial reproductive mycelium and secondary metabolites. We observed novel phenomena such as an extended life cycle including flotation and anaerobiosis using standing liquid cultures. This paper describes an improved method for isolating good quality RNA from standing liquid cultures of S. coelicolor via excellent cell lysis.


Subject(s)
RNA, Bacterial/isolation & purification , Streptomyces/genetics , Blotting, Northern , Deoxyribonuclease I/chemistry , Guanidines/chemistry , Isothiocyanates/chemistry , Phenol/chemistry , Spores, Bacterial , Streptomyces/chemistry
7.
Genes Dev ; 17(14): 1714-26, 2003 Jul 15.
Article in English | MEDLINE | ID: mdl-12832396

ABSTRACT

Streptomycetes exhibit a complex morphological differentiation. After a submerged mycelium has been formed, filaments grow into the air to septate into spores. A class of eight hydrophobic secreted proteins, ChpA-H, was shown to be instrumental in the development of Streptomyces coelicolor. Mature forms of ChpD-H are up to 63 amino acids in length, and those of ChpA-C are larger (+/-225 amino acids). ChpA-C contain two domains similar to ChpD-H, as well as a cell-wall sorting signal. The chp genes were expressed in submerged mycelium (chpE and chpH) as well as in aerial hyphae (chpA-H). Formation of aerial hyphae was strongly affected in a strain in which six chp genes were deleted (DeltachpABCDEH). A mixture of ChpD-H purified from cell walls of aerial hyphae complemented the DeltachpABCDEH strain extracellularly, and it accelerated development in the wild-type strain. The protein mixture was highly surface active, and it self-assembled into amyloid-like fibrils at the water-air interface. The fibrils resembled those of a surface layer of aerial hyphae. We thus conclude that the amyloid-like fibrils of ChpD-H lower the water surface tension to allow aerial growth and cover aerial structures, rendering them hydrophobic. ChpA-C possibly bind ChpD-H to the cell wall.


Subject(s)
Hydrophobic and Hydrophilic Interactions , Hyphae/growth & development , Proteins/metabolism , Streptomyces/growth & development , Amino Acid Sequence , Molecular Sequence Data
SELECTION OF CITATIONS
SEARCH DETAIL
...