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1.
Am J Physiol Lung Cell Mol Physiol ; 301(2): L228-35, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21622847

ABSTRACT

Recent studies proposed that mechanical inactivity of the human diaphragm during mechanical ventilation rapidly causes diaphragm atrophy and weakness. However, conclusive evidence for the notion that diaphragm weakness is a direct consequence of mechanical inactivity is lacking. To study the effect of hemidiaphragm paralysis on diaphragm muscle fiber function and structure in humans, biopsies were obtained from the paralyzed hemidiaphragm in eight patients with hemidiaphragm paralysis. All patients had unilateral paralysis of known duration, caused by en bloc resection of the phrenic nerve with a tumor. Furthermore, diaphragm biopsies were obtained from three control subjects. The contractile performance of demembranated muscle fibers was determined, as well as fiber ultrastructure and morphology. Finally, expression of E3 ligases and proteasome activity was determined to evaluate activation of the ubiquitin-proteasome pathway. The force-generating capacity, as well as myofibrillar ultrastructure, of diaphragm muscle fibers was preserved up to 8 wk of paralysis. The cross-sectional area of slow fibers was reduced after 2 wk of paralysis; that of fast fibers was preserved up to 8 wk. The expression of the E3 ligases MAFbx and MuRF-1 and proteasome activity was not significantly upregulated in diaphragm fibers following paralysis, not even after 72 and 88 wk of paralysis, at which time marked atrophy of slow and fast diaphragm fibers had occurred. Diaphragm muscle fiber atrophy and weakness following hemidiaphragm paralysis develops slowly and takes months to occur.


Subject(s)
Diaphragm/pathology , Diaphragm/physiopathology , Muscle Fibers, Skeletal/pathology , Paralysis/diagnosis , Paralysis/physiopathology , Aged , Anatomy, Cross-Sectional , Diaphragm/diagnostic imaging , Female , Humans , Immunohistochemistry , Male , Microscopy, Electron , Middle Aged , Muscle Contraction , Muscle Fibers, Fast-Twitch , Muscle Fibers, Skeletal/enzymology , Muscle Fibers, Slow-Twitch , Muscle Proteins/metabolism , Muscle Weakness/etiology , Muscle Weakness/physiopathology , Muscular Atrophy/etiology , Muscular Atrophy/pathology , Paralysis/complications , Paralysis/etiology , Phrenic Nerve/surgery , Postoperative Complications , Proteasome Endopeptidase Complex , Radiography, Thoracic , SKP Cullin F-Box Protein Ligases/metabolism , Time Factors , Tomography, X-Ray Computed , Tripartite Motif Proteins , Ubiquitin-Protein Ligases/metabolism
2.
Am J Physiol Lung Cell Mol Physiol ; 299(6): L898-904, 2010 Dec.
Article in English | MEDLINE | ID: mdl-20817779

ABSTRACT

We present a systematic quantitative analysis of power-law force relaxation and investigate logarithmic superposition of force response in relaxed porcine airway smooth muscle (ASM) strips in vitro. The term logarithmic superposition describes linear superposition on a logarithmic scale, which is equivalent to multiplication on a linear scale. Additionally, we examine whether the dynamic response of contracted and relaxed muscles is dominated by cross-bridge cycling or passive dynamics. The study shows the following main findings. For relaxed ASM, the force response to length steps of varying amplitude (0.25-4% of reference length, both lengthening and shortening) are well-fitted with power-law functions over several decades of time (10⁻² to 10³ s), and the force response after consecutive length changes is more accurately fitted assuming logarithmic superposition rather than linear superposition. Furthermore, for sinusoidal length oscillations in contracted and relaxed muscles, increasing the oscillation amplitude induces greater hysteresivity and asymmetry of force-length relationships, whereas increasing the frequency dampens hysteresivity but increases asymmetry. We conclude that logarithmic superposition is an important feature of relaxed ASM, which may facilitate a more accurate prediction of force responses in the continuous dynamic environment of the respiratory system. In addition, the single power-function response to length changes shows that the dynamics of cross-bridge cycling can be ignored in relaxed muscle. The similarity in response between relaxed and contracted states implies that the investigated passive dynamics play an important role in both states and should be taken into account.


Subject(s)
Muscle Contraction/physiology , Muscle, Smooth/physiology , Stress, Mechanical , Trachea , Animals , Models, Biological , Muscle Relaxation/physiology , Muscle, Smooth/anatomy & histology , Sus scrofa , Temperature , Trachea/anatomy & histology , Trachea/physiology
3.
Kidney Blood Press Res ; 31(3): 171-84, 2008.
Article in English | MEDLINE | ID: mdl-18483460

ABSTRACT

Although primary cilia are increasingly recognized to play sensory roles in several cellular systems, their role in vascular smooth muscle cells (VSMCs) has not been defined. We examined in situ position/orientation of primary cilia and ciliary proteins in VSMCs and tested the hypothesis that primary cilia of VSMCs exert sensory functions. By immunofluorescence and electron microscopic imaging, primary cilia of VSMCs were positioned with their long axis aligned at 58.3 degrees angle in relation to the cross-sectional plane of the artery, projecting into the extracellular matrix (ECM). Polycystin-1, polycystin-2 and alpha 3- and beta1-integrins are present in cilia. In scratch wound experiments, the majority of cilia were repositioned to the cell-wound interface. Such repositioning was largely abolished by a beta1-integrin blocker. Moreover, compared to non-ciliated/deciliated cells, ciliated VSMCs showed more efficient migration in wound repair. Lastly, when directly stimulated with collagen (an ECM component and cognate ligand for alpha 3beta1-integrins) or induced ciliary deflection, VSMCs responded with a rise in [Ca(2+)](i) that is dependent on the presence of cilia. Taken together, primary cilia of VSMCs are preferentially oriented, possess proteins critical for cell-ECM interaction and mechanosensing and respond to ECM protein and mechanical stimulations. These observations suggest a role for primary cilia in mechanochemical sensing in vasculature.


Subject(s)
Cilia/pathology , Cilia/physiology , Muscle, Smooth, Vascular/pathology , Animals , Aorta , Cilia/chemistry , Collagen/pharmacology , Integrin beta1/analysis , Mechanotransduction, Cellular , Mice , Microscopy , Myocytes, Smooth Muscle/pathology , Wound Healing
4.
Neuroscience ; 146(1): 178-89, 2007 Apr 25.
Article in English | MEDLINE | ID: mdl-17346898

ABSTRACT

Both spinal hemisection (SH) at C2 and tetrodotoxin (TTX) phrenic nerve blockade result in diaphragm muscle paralysis and inactivity of the phrenic axon terminals. However, phrenic motoneuron somata are inactive with SH but remain active with TTX phrenic nerve blockade. Neuromuscular transmission failure with repeated activation decreases following SH and increases following TTX phrenic nerve blockade, suggesting that matching (or mismatching) of somal and synaptic inactivities of phrenic motoneurons differentially regulates synaptic vesicle pools at diaphragm neuromuscular junctions. At individual type-identified rat diaphragm presynaptic terminals, the size of the releasable pool of synaptic vesicles was analyzed by fluorescence confocal microscopy of N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl) pyridinium dibromide (FM4-64) uptake and synaptic vesicle density at active zones was determined using transmission electron microscopy. After 14 days of SH and TTX-induced diaphragm muscle inactivity, neuromuscular junction size was not different at type I or IIa fibers, but increased at type IIx and/or IIb fibers (by 51% in SH and 35% in TTX) compared with control. With SH, synaptic vesicle pool size and density increased at presynaptic terminals innervating type I or IIa fibers (17 and 63%, respectively; P<0.001) and type IIx and/or IIb fibers (41 and 31%, respectively; P<0.001) when compared with controls. Following TTX, synaptic vesicle pool size and density decreased by 64 and 17%, respectively, at presynaptic terminals innervating type I or IIa fibers, and by 50 and 36%, respectively, at type IIx and/or IIb fibers (P<0.001, for all comparisons). Thus, matching motoneuron soma and axon terminal inactivity (SH) increases the size and density of releasable synaptic vesicle pools at adult rat diaphragm neuromuscular junctions. Mismatching motoneuron soma and axon terminal inactivities (TTX) results in converse presynaptic adaptations. Inactivity-induced neuromuscular plasticity reflects specific adaptations in the size and density of synaptic vesicle pools that depend on motoneuron soma rather than axon terminal (or muscle fiber) inactivity.


Subject(s)
Diaphragm/cytology , Motor Neurons/physiology , Neuromuscular Junction/physiology , Presynaptic Terminals/physiology , Synaptic Vesicles/physiology , Anesthetics, Local/pharmacology , Animals , In Vitro Techniques , Male , Microscopy, Electron, Transmission/methods , Muscle Fibers, Skeletal/pathology , Muscle Fibers, Skeletal/ultrastructure , Phrenic Nerve/drug effects , Phrenic Nerve/physiology , Presynaptic Terminals/ultrastructure , Pyridinium Compounds/metabolism , Quaternary Ammonium Compounds/metabolism , Rats , Rats, Sprague-Dawley , Spinal Cord Injuries/pathology , Spinal Cord Injuries/physiopathology , Synaptic Vesicles/ultrastructure , Tetrodotoxin/pharmacology
5.
Biofizika ; 51(5): 894-7, 2006.
Article in Russian | MEDLINE | ID: mdl-17131830

ABSTRACT

The effect of phalloidin, an agent detaching nebulin from actin in skeletal muscle, on the isometric force in lamprey skinned cardiac muscle, which has nebulin in amounts comparable to that in skeletal muscle, has been studied. We found that, unlike mammalian cardiac muscle expressing nebulin less abundantly and responding to phalloidin by a force increase, lamprey cardiac muscle responds to phalloidin by a force decrease (approximately 50% decrease), thereby resembling the response of skeletal muscle. These results support our hypothesis that nebulin detachment from actin underlies phalloidin-induced force loss and suggest a role of actin-nebulin interaction in contractile function.


Subject(s)
Lampreys/physiology , Muscle Proteins/metabolism , Myocardial Contraction , Myocardium/metabolism , Phalloidine/metabolism , Animals , In Vitro Techniques , Isometric Contraction , Phalloidine/pharmacology
6.
J Appl Physiol (1985) ; 91(5): 2117-24, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11641352

ABSTRACT

In the present study, we used real-time confocal microscopy to examine the effects of two nitric oxide (NO) donors on acetylcholine (ACh; 10 microM)- and caffeine (10 mM)-induced intracellular calcium concentration ([Ca2+]i) responses in C2C12 mouse skeletal myotubes. We hypothesized that NO reduces [Ca2+]i in activated skeletal myotubes through oxidation of thiols associated with the sarcoplasmic reticulum Ca2+-release channel. Exposure to diethylamine NONOate (DEA-NO) reversibly increased resting [Ca2+]i level and resulted in a dose-dependent reduction in the amplitude of ACh-induced [Ca2+]i responses (25 +/- 7% reduction with 10 microM DEA-NO and 78 +/- 14% reduction with 100 microM DEA-NO). These effects of DEA-NO were partly reversible after subsequent exposure to dithiothreitol (10 mM). Preexposure to DEA-NO (1, 10, and 50 microM) also reduced the amplitude of the caffeine-induced [Ca2+]i response. Similar data were obtained by using the chemically distinct NO donor S-nitroso-N-acetyl-penicillamine (100 microM). These results indicate that NO reduces sarcoplasmic reticulum Ca2+ release in skeletal myotubes, probably by a modification of hyperreactive thiols present on the ryanodine receptor channel.


Subject(s)
Calcium/metabolism , Microtubules/metabolism , Muscle, Skeletal/metabolism , Nitric Oxide/pharmacology , Sarcoplasmic Reticulum/metabolism , Animals , Caffeine/pharmacology , Cells, Cultured , Dithiothreitol/pharmacology , Image Processing, Computer-Assisted , Macrocyclic Compounds , Mice , Microscopy, Confocal , Microtubules/drug effects , Muscle, Skeletal/drug effects , Nitric Oxide Donors/pharmacology , Oxazoles/pharmacology , Oxidation-Reduction , Phosphodiesterase Inhibitors/pharmacology , Ryanodine/pharmacology , Ryanodine Receptor Calcium Release Channel/drug effects , Ryanodine Receptor Calcium Release Channel/metabolism , Sarcoplasmic Reticulum/drug effects , Sulfhydryl Compounds/metabolism
7.
J Appl Physiol (1985) ; 91(5): 2233-9, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11641366

ABSTRACT

The effects of the nitric oxide (NO) donor spermine NONOate (Sp-NO, 1.0 mM) on cross-bridge recruitment and cross-bridge cycling kinetics were studied in permeabilized rabbit psoas muscle fibers. Fibers were activated at various Ca2+ concentrations (pCa, negative logarithm of Ca2+ concentration), and the pCa at which force was maximal (pCa 4.0) and approximately 50% of maximal (pCa50 5.6) were determined. Fiber stiffness was determined using 1-kHz sinusoidal length perturbations, and the fraction of cross bridges in the force-generating state was estimated by the ratio of stiffness during maximal (pCa 4.0) and submaximal (pCa 5.6) Ca2+ activation to stiffness during rigor (at pCa 4.0). Cross-bridge cycling kinetics were evaluated by measuring the rate constant for force redevelopment after quick release (by 15% of optimal fiber length, L(o)) and restretch of the fiber to L(o). Exposing fibers to Sp-NO for 10 min reduced force and the fraction of cross bridges in the force-generating state at maximal and submaximal (pCa50) Ca2+ activation. However, the effects of Sp-NO were more pronounced during submaximal Ca2+ activation. Sp-NO also reduced the rate constant for force redevelopment but only during submaximal Ca2+ activation. We conclude that Sp-NO reduces Ca2+ sensitivity by decreasing the number of cross bridges in the strongly bound state and also impairs cross-bridge cycling kinetics during submaximal activation.


Subject(s)
Calcium/physiology , Muscle, Skeletal/metabolism , Nitric Oxide/pharmacology , Algorithms , Animals , Biotransformation/drug effects , In Vitro Techniques , Kinetics , Muscle Contraction/physiology , Muscle Fibers, Skeletal/drug effects , Muscle Fibers, Skeletal/physiology , Muscle, Skeletal/cytology , Muscle, Skeletal/drug effects , Permeability , Rabbits
8.
J Appl Physiol (1985) ; 91(5): 2266-74, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11641370

ABSTRACT

In airway smooth muscle (ASM), ACh induces propagating intracellular Ca2+ concentration ([Ca2+]i) oscillations (5-30 Hz). We hypothesized that, in ASM, coupling of elevations and reductions in [Ca2+]i to force generation and relaxation (excitation-contraction coupling) is slower than ACh-induced [Ca2+]i oscillations, leading to stable force generation. When we used real-time confocal imaging, the delay between elevated [Ca2+]i and contraction in intact porcine ASM cells was found to be approximately 450 ms. In beta-escin-permeabilized ASM strips, photolytic release of caged Ca2+ resulted in force generation after approximately 800 ms. When calmodulin (CaM) was added, this delay was shortened to approximately 500 ms. In the presence of exogenous CaM and 100 microM Ca2+, photolytic release of caged ATP led to force generation after approximately 80 ms. These results indicated significant delays due to CaM mobilization and Ca2+-CaM activation of myosin light chain kinase but much shorter delays introduced by myosin light chain kinase-induced phosphorylation of the regulatory myosin light chain MLC20 and cross-bridge recruitment. This was confirmed by prior thiophosphorylation of MLC20, in which force generation occurred approximately 50 ms after photolytic release of caged ATP, approximating the delay introduced by cross-bridge recruitment alone. The time required to reach maximum steady-state force was >15 s. Rapid chelation of [Ca2+]i after photolytic release of caged diazo-2 resulted in relaxation after a delay of approximately 1.2 s and 50% reduction in force after approximately 57 s. We conclude that in ASM cells agonist-induced [Ca2+]i oscillations are temporally and spatially integrated during excitation-contraction coupling, resulting in stable force production.


Subject(s)
Muscle, Smooth/physiology , Trachea/physiology , Acetylcholine/pharmacology , Animals , Calcium/metabolism , In Vitro Techniques , Muscle Contraction/physiology , Muscle Relaxation/physiology , Muscle, Smooth/cytology , Muscle, Smooth/metabolism , Perfusion , Photolysis , Solutions , Swine , Time Factors , Trachea/cytology , Trachea/metabolism
9.
Endothelium ; 8(2): 137-45, 2001.
Article in English | MEDLINE | ID: mdl-11572475

ABSTRACT

Endothelin-1 is an endothelium-derived factor which alters tone and proliferation of vascular smooth muscle and has been implicated in the development of atherosclerosis. Estrogen modulates production of and contractile responses to endothelin-1. Since atherosclerosis is less in estrogen-replete women compared to men, experiments were designed to determine whether or not there were gender-associated differences in proliferative responses to endothelin-1 and effect of estrogen status on those responses. Proliferation of smooth muscle cells derived from coronary arteries of sexually mature, gondally intact male and female and oophorectomized female pigs was determined by thymidine incorporation in the absence and presence of endothelin-1 with and without 17beta-estradiol. Endothelin-1 (10(-9) M to 10(-7) M) significantly inhibited proliferation only in coronary smooth muscle cells from intact female pigs. Addition of beta-estradiol inhibited proliferation of cells from intact females but there was not a synergistic effect with endothelin-1. Gender associated inhibition of smooth muscle proliferation by endothelin-1 may contribute, in part, to cardioprotection noted in estrogen-replete states.


Subject(s)
Coronary Vessels/cytology , Endothelin-1/pharmacology , Muscle, Smooth, Vascular/metabolism , Animals , Cell Division/drug effects , Cells, Cultured , Coronary Vessels/drug effects , Dihydrotestosterone/pharmacology , Dose-Response Relationship, Drug , Drug Interactions , Estradiol/pharmacology , Female , Immunochemistry , Male , Muscle, Smooth, Vascular/cytology , Muscle, Smooth, Vascular/drug effects , Ovariectomy , Phenotype , Pulmonary Artery/cytology , Pulmonary Artery/drug effects , Sex Factors , Swine
11.
Anesthesiology ; 95(1): 207-15, 2001 Jul.
Article in English | MEDLINE | ID: mdl-11465560

ABSTRACT

BACKGROUND: Volatile anesthetics relax airway smooth muscle (ASM) by altering intracellular Ca2+ concentration ([Ca2+]i). The authors hypothesized that relaxation is produced by decreasing sarcoplasmic reticulum Ca2+ content via increased Ca2+ "leak" through both inositol trisphosphate (IP3) and ryanodine receptor channels. METHODS: Enzymatically dissociated porcine ASM cells were exposed to acetylcholine in the presence or absence of 2 minimum alveolar concentration (MAC) halothane, and IP3 levels were measured using radioimmunoreceptor assay. Other cells were loaded with the Ca2+ indicator fluo-3 and imaged using real-time confocal microscopy. RESULTS: Halothane increased IP3 concentrations in the presence and absence of acetylcholine. Inhibition of phospholipase C blunted the IP3 response to halothane. Exposure to 2 MAC halothane induced a transient [Ca2+]i response, suggesting depletion of sarcoplasmic reticulum Ca2+. Exposure to 20 microM Xestospongin D, a cell-permeant IP3 receptor antagonist, resulted in a 45+/-13% decrease in the [Ca2+]i response to halothane compared with halothane exposure alone. In permeabilized cells, Xestospongin D or 0.5 mg/ml heparin decreased the [Ca2+]i response to halothane by 65+/-13% and 68+/-22%, respectively, compared with halothane alone. In both intact and permeabilized cells, 20 microM ryanodine blunted the [Ca2+]i response to halothane by 32+/-13% and 39+/-21%, respectively, compared with halothane alone. Simultaneous exposure to Xestospongin D and ryanodine completely inhibited the [Ca2+]i response to halothane. CONCLUSIONS: The authors conclude that halothane reduces sarcoplasmic reticulum Ca2+ content in ASM cells via increased Ca2+ leak through both IP3 receptor and ryanodine receptor channels. Effects on IP3 receptor channels are both direct and indirect via elevation of IP3 levels.


Subject(s)
Anesthetics, Inhalation/pharmacology , Calcium Channels/metabolism , Halothane/pharmacology , Muscle, Smooth/metabolism , Sarcoplasmic Reticulum/metabolism , Animals , Calcium Channels/drug effects , Calibration , Escin/pharmacology , In Vitro Techniques , Inositol 1,4,5-Trisphosphate/metabolism , Inositol 1,4,5-Trisphosphate Receptors , Microscopy, Confocal , Muscle, Smooth/cytology , Muscle, Smooth/drug effects , Receptors, Cytoplasmic and Nuclear/drug effects , Receptors, Cytoplasmic and Nuclear/metabolism , Ryanodine Receptor Calcium Release Channel/drug effects , Ryanodine Receptor Calcium Release Channel/metabolism , Sarcoplasmic Reticulum/drug effects , Swine
12.
Arterioscler Thromb Vasc Biol ; 21(6): 1017-22, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11397713

ABSTRACT

Endothelium-dependent relaxations mediated by NO are impaired in a mouse model of human atherosclerosis. Our objective was to characterize the mechanisms underlying endothelial dysfunction in aortas of apolipoprotein E (apoE)-deficient mice, treated for 26 to 29 weeks with a lipid-rich Western-type diet. Aortic rings from apoE-deficient mice showed impaired endothelium-dependent relaxations to acetylcholine (10(-)(9) to 10(-)(5) mol/L) and Ca(2+) ionophore (10(-)(9) to 10(-)(6) mol/L) and endothelium-independent relaxations to diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA-NONOate, 10(-)(10) to 10(-)(5) mol/L) compared with aortic rings from C57BL/6J mice (P<0.05). By use of confocal microscopy of an oxidative fluorescent probe (dihydroethidium), increased superoxide anion (O(2)(-)) production was demonstrated throughout the aortic wall but mainly in smooth muscle cells of apoE-deficient mice. CuZn-superoxide dismutase (SOD) and Mn-SOD protein expressions were unaltered in the aorta exposed to hypercholesterolemia. A cell-permeable SOD mimetic, Mn(III) tetra(4-benzoic acid) porphyrin chloride (10(-)(5) mol/L), reduced O(2)(-) production and partially normalized relaxations to acetylcholine and DEA-NONOate in apoE-deficient mice (P<0.05). [(14)C]L-Citrulline assay showed a decrease of Ca(2+)-dependent NOS activity in aortas from apoE-deficient mice compared with C57BL/6J mice (P<0.05), whereas NO synthase protein expression was unchanged. In addition, cGMP levels were significantly reduced in the aortas of apoE-deficient mice (P<0.05). Our results demonstrate that in apoE-deficient mice on a Western-type fat diet, impairment of endothelial function is caused by increased production of O(2)(-) and reduced endothelial NO synthase enzyme activity. Thus, chemical inactivation of NO with O(2)(-) and reduced biosynthesis of NO are key mechanisms responsible for endothelial dysfunction in aortas of atherosclerotic apoE-deficient mice.


Subject(s)
Apolipoproteins E/genetics , Arteriosclerosis/physiopathology , Endothelium, Vascular/metabolism , Animals , Aorta/drug effects , Aorta/metabolism , Aorta/physiopathology , Arteriosclerosis/metabolism , Blotting, Western , Calcium/metabolism , Culture Techniques , Cyclic AMP/biosynthesis , Cyclic GMP/biosynthesis , Male , Metalloporphyrins/pharmacology , Mice , Mice, Inbred C57BL , Mice, Knockout , Nitric Oxide Synthase/metabolism , Nitric Oxide Synthase Type II , Nitric Oxide Synthase Type III , Superoxide Dismutase/immunology , Superoxide Dismutase/metabolism , Superoxides/metabolism , Vasoconstriction , Vasodilation
13.
J Appl Physiol (1985) ; 91(1): 488-96, 2001 Jul.
Article in English | MEDLINE | ID: mdl-11408467

ABSTRACT

The multiplicity of mechanisms involved in regulation of intracellular Ca(2+) concentration ([Ca(2+)](i)) in smooth muscle results in both intra- and intercellular heterogeneities in [Ca(2+)](i). Heterogeneity in [Ca(2+)](i) regulation is reflected by the presence of spontaneous, localized [Ca(2+)](i) transients (Ca(2+) sparks) representing Ca(2+) release through ryanodine receptor (RyR) channels. Ca(2+) sparks display variable spatial Ca(2+) distributions with every occurrence within and across cellular regions. Individual sparks are often grouped, and fusion of sparks produces large local elevations in [Ca(2+)](i) that occasionally trigger propagating [Ca(2+)](i) waves. Ca(2+) sparks may modulate membrane potential and thus smooth muscle contractility. Sparks may also be the target of other regulatory factors in smooth muscle. Agonists induce propagating [Ca(2+)](i) oscillations that originate from foci with high spark incidence and also represent Ca(2+) release through RyR channels. With increasing agonist concentration, the peak of regional [Ca(2+)](i) oscillations remains relatively constant, whereas both frequency and propagation velocity increase. In contrast, the global cellular response appears as a concentration-dependent increase in peak as well as mean cellular [Ca(2+)](i), representing a spatial and temporal integration of the oscillations. The significance of agonist-induced [Ca(2+)](i) oscillations lies in the establishment of a global [Ca(2+)](i) level for slower Ca(2+)-dependent physiological processes.


Subject(s)
Calcium/metabolism , Muscle, Smooth/metabolism , Animals , Oscillometry , Time Factors , Tissue Distribution
14.
J Physiol ; 532(Pt 1): 91-104, 2001 Apr 01.
Article in English | MEDLINE | ID: mdl-11283227

ABSTRACT

We examined the influence of two clinically relevant concentrations (1 and 2 MAC (minimum alveolar concentration)) of halothane and sevoflurane on both efflux and reverse modes of Na+-Ca2+ exchange (NCX) in enzymatically dissociated adult rat cardiac myocytes. We hypothesised that a volatile anaesthetic-induced decrease in myocardial contractility is mediated by a reduction in intracellular calcium concentration ([Ca2+]i) via inhibition of NCX. Cells were exposed to cyclopiazonic acid and zero extracellular Na+ and Ca2+ to block sacroplasmic reticulum (SR) re-uptake and NCX efflux, respectively. As [Ca2+]i increased under these conditions, extracellular Na+ was rapidly (< 300 ms) reintroduced in the presence or absence of a volatile anaesthetic to selectively promote Ca2+ efflux via NCX. Other cells exposed to cyclopiazonic acid and ryanodine to inhibit SR Ca2+ re-uptake and release were Na+ loaded in zero extracellular Ca2+. The reintroduction of extracellular Ca2+ was used to selectively activate Ca2+ influx via NCX. Compared to controls, both 1 and 2 MAC halothane as well as sevoflurane reduced NCX-mediated efflux. The reduction in NCX-mediated influx was concentration dependent, but comparable between the two anaesthetics. Both anaesthetics at each concentration also shifted the relationship between extracellular Na+ (or extent of Na+ loading) and NCX-mediated efflux (or influx) to the right. These data indicate that despite inhibition of NCX-mediated Ca2+ efflux, volatile anaesthetics produce myocardial depression. However, the inhibition of NCX-mediated Ca2+ influx may contribute to decreased cardiac contractility. The overall effect of volatile anaesthetics on the [Ca2+]i profile is likely to be determined by the relative contributions of influx vs. efflux via NCX during each cardiac cycle.


Subject(s)
Anesthetics, Inhalation/pharmacology , Calcium/metabolism , Halothane/pharmacology , Methyl Ethers/pharmacology , Myocardium/metabolism , Sodium-Calcium Exchanger/metabolism , Sodium/metabolism , Animals , Anti-Arrhythmia Agents/pharmacology , Cells, Cultured , Dose-Response Relationship, Drug , Heart/drug effects , Heart/physiology , Indoles/pharmacology , Male , Microscopy, Confocal , Myocardium/cytology , Rats , Rats, Sprague-Dawley , Ryanodine/pharmacology , Sarcoplasmic Reticulum/drug effects , Sarcoplasmic Reticulum/metabolism , Sevoflurane
15.
J Appl Physiol (1985) ; 90(4): 1196-204, 2001 Apr.
Article in English | MEDLINE | ID: mdl-11247914

ABSTRACT

We hypothesize that 1) the effect of denervation (DNV) is more pronounced in fibers expressing fast myosin heavy chain (MHC) isoforms and 2) the effect of DNV on maximum specific force reflects a reduction in MHC content per half sarcomere or the number of cross bridges in parallel. Studies were performed on single Triton X-100-permeabilized fibers activated at a pCa (-log Ca2+ concentration) of 4.0. MHC content per half sarcomere was determined by densitometric analysis of SDS-PAGE gels and comparison to a standard curve of known MHC concentrations. After 2 of wk DNV, the maximum specific force of fibers expressing MHC2X was reduced by approximately 40% (MHC(2B) expression was absent), whereas the maximum specific force of fibers expressing MHC2A and MHC(slow) decreased by only approximately 20%. DNV also reduced the MHC content in fibers expressing MHC2X, with no effect on fibers expressing MHC2A and MHC(slow). When normalized for MHC content per half sarcomere, force generated by DNV fibers expressing MHC2X and MHC2A was decreased compared with control fibers. These results suggest the force per cross bridge is also affected by DNV.


Subject(s)
Diaphragm/physiology , Muscle Fibers, Skeletal/physiology , Animals , Blotting, Western , Diaphragm/innervation , Diaphragm/metabolism , Isomerism , Male , Muscle Denervation , Muscle Fibers, Skeletal/metabolism , Myosin Heavy Chains/biosynthesis , Rats , Rats, Sprague-Dawley , Sarcomeres/metabolism
16.
J Appl Physiol (1985) ; 90(3): 850-6, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11181592

ABSTRACT

The effect of chronic exogenous testosterone (T) treatment on neuromuscular transmission in the diaphragm (Dia) muscle of adult male rats was determined. The contribution of neuromuscular transmission failure (NTF) to Dia fatigue was evaluated by superimposing intermittent direct muscle stimulation on repetitive nerve stimulation of isometric contraction in vitro. T treatment significantly reduced the contribution of NTF to Dia fatigue by approximately 20% (P < 0.001). Fiber type-specific effects on NTF were determined by measuring Dia fiber glycogen levels subsequent to repetitive nerve or muscle stimulation. T treatment had no effect on glycogen depletion in Dia type I and IIa fibers regardless of stimulation route. In the control group, type IIx fibers demonstrated significantly less glycogen depletion after nerve stimulation compared with direct muscle stimulation (P < 0.05), suggesting the presence of NTF. In contrast, T treatment increased glycogen depletion of type IIx fibers during nerve stimulation to levels similar to those after direct muscle stimulation. These data indicate that testosterone treatment substantially improves neuromuscular transmission in the Dia.


Subject(s)
Diaphragm/physiology , Neuromuscular Junction/physiology , Testosterone/pharmacology , Animals , Diaphragm/drug effects , Diaphragm/innervation , Drug Implants , Electric Stimulation , Glycogen/metabolism , In Vitro Techniques , Isometric Contraction/drug effects , Isometric Contraction/physiology , Male , Muscle Fatigue , Muscle Fibers, Skeletal/physiology , Muscle, Skeletal/drug effects , Muscle, Skeletal/innervation , Muscle, Skeletal/physiology , Neuromuscular Junction/drug effects , Rats , Rats, Sprague-Dawley , Synaptic Transmission/drug effects , Testosterone/administration & dosage
17.
J Appl Physiol (1985) ; 90(3): 1158-64, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11181631

ABSTRACT

Numerous studies have explored the energetic properties of skeletal and cardiac muscle fibers. In this mini-review, we specifically explore the interactions between actin and myosin during cross-bridge cycling and provide a conceptual framework for the chemomechanical transduction that drives muscle fiber energetic demands. Because the myosin heavy chain (MHC) is the site of ATP hydrolysis and actin binding, we focus on the mechanical and energetic properties of different MHC isoforms. Based on the conceptual framework that is provided, we discuss possible sites where muscle remodeling may impact the energetic demands of contraction in skeletal and cardiac muscle.


Subject(s)
Heart/physiology , Muscle Contraction/physiology , Muscle, Skeletal/physiology , Myocardial Contraction/physiology , Actins/metabolism , Actomyosin/metabolism , Adenosine Triphosphate/metabolism , Animals , Humans , Muscle Fibers, Skeletal/physiology , Myosin Heavy Chains/metabolism
18.
J Appl Physiol (1985) ; 90(2): 657-64, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11160066

ABSTRACT

Maximum velocity of the actomyosin ATPase reaction (V(max) ATPase) and ATP consumption rate during maximum isometric activation (ATP(iso)) were determined in human vastus lateralis (VL) muscle fibers expressing different myosin heavy chain (MHC) isoforms. We hypothesized that the reserve capacity for ATP consumption [1 -- (ratio of ATP(iso) to V(max) ATPase)] varies across VL muscle fibers expressing different MHC isoforms. Biopsies were obtained from 12 subjects (10 men and 2 women; age 21--66 yr). A quantitative histochemical procedure was used to measure V(max) ATPase. In permeabilized fibers, ATP(iso) was measured using an NADH-linked fluorometric procedure. The reserve capacity for ATP consumption was lower for fibers coexpressing MHC(2X) and MHC(2A) compared with fibers singularly expressing MHC(2A) and MHC(slow) (39 vs. 52 and 56%, respectively). Tension cost (ratio of ATP(iso) to generated force) also varied with fiber type, being highest in fibers coexpressing MHC(2X) and MHC(2A). We conclude that fiber-type differences in the reserve capacity for ATP consumption and tension cost reflect functional differences such as susceptibility to fatigue.


Subject(s)
Adenosine Triphosphate/metabolism , Isometric Contraction , Muscle Fibers, Skeletal/metabolism , Muscle, Skeletal/metabolism , Adenosine Triphosphatases/metabolism , Adult , Aged , Electrophoresis, Polyacrylamide Gel , Female , Gene Expression , Humans , Kinetics , Male , Middle Aged , Muscle Fibers, Skeletal/classification , Muscle, Skeletal/physiology , Myosin Heavy Chains/metabolism , Protein Isoforms/metabolism , Temperature
19.
J Appl Physiol (1985) ; 90(1): 380-8, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11133931

ABSTRACT

It has been found that maximum specific force (F(max); force per cross-sectional area) of rat diaphragm muscle doubles from birth to 84 days (adult). We hypothesize that this developmental change in F(max) reflects an increase in myosin heavy chain (MHC) content per half-sarcomere (an estimate of the number of cross bridges in parallel) and/or a greater force per cross bridge in fibers expressing fast MHC isoforms compared with slow and neonatal MHC isoforms (MHC(slow) and MHC(neo), respectively). Single Triton 100-X-permeabilized fibers were activated at a pCa of 4.0. MHC isoform expression was determined by SDS-PAGE. MHC content per half-sarcomere was determined by densitometric analysis and comparison to a standard curve of known MHC concentrations. MHC content per half-sarcomere progressively increased during early postnatal development. When normalized for MHC content per half-sarcomere, fibers expressing MHC(slow) and coexpressing MHC(neo) produced less force than fibers expressing fast MHC isoforms. We conclude that lower force per cross bridge in fibers expressing MHC(slow) and MHC(neo) contributes to the lower F(max) seen in early postnatal development.


Subject(s)
Aging/physiology , Diaphragm/physiology , Muscle Contraction/physiology , Muscle Fibers, Skeletal/physiology , Animals , Animals, Newborn/physiology , Male , Muscle Fibers, Skeletal/metabolism , Muscle Fibers, Skeletal/ultrastructure , Myosin Heavy Chains/metabolism , Protein Isoforms/metabolism , Rats , Rats, Sprague-Dawley , Sarcomeres/metabolism
20.
J Appl Physiol (1985) ; 89(6): 2215-9, 2000 Dec.
Article in English | MEDLINE | ID: mdl-11090570

ABSTRACT

We hypothesized that decrements in maximum power output (W(max)) of the rat diaphragm (Dia) muscle with repetitive activation are due to a disproportionate reduction in force (force fatigue) compared with a slowing of shortening velocity (velocity fatigue). Segments of midcostal Dia muscle were mounted in vitro (26 degrees C) and stimulated directly at 75 Hz in 400-ms-duration trains repeated each second (duty cycle = 0.4) for 120 s. A novel technique was used to monitor instantaneous reductions in maximum specific force (P(o)) and W(max) during fatigue. During each stimulus train, activation was isometric for the initial 360 ms during which P(o) was measured; the muscle was then allowed to shorten at a constant velocity (30% V(max)) for the final 40 ms, and W(max) was determined. Compared with initial values, after 120 s of repetitive activation, P(o) and W(max) decreased by 75 and 73%, respectively. Maximum shortening velocity was measured in two ways: by extrapolation of the force-velocity relationship (V(max)) and using the slack test [maximum unloaded shortening velocity (V(o))]. After 120 s of repetitive activation, V(max) slowed by 44%, whereas V(o) slowed by 22%. Thus the decrease in W(max) with repetitive activation was dominated by force fatigue, with velocity fatigue playing a secondary role. On the basis of a greater slowing of V(max) vs. V(o), we also conclude that force and power fatigue cannot be attributed simply to the total inactivation of the most fatigable fiber types.


Subject(s)
Diaphragm/physiology , Muscle Fatigue/physiology , Animals , Electric Stimulation , In Vitro Techniques , Isometric Contraction/physiology , Male , Rats , Rats, Sprague-Dawley , Time Factors
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