ABSTRACT
The increased analytical sensitivity of capillary electrophoresis detects additional irregularities that are suspicious for a monoclonal component. This is most noticeable in the beta-1-, beta-2- and gamma-globulin fractions. The causes of non-monoclonal irregularities are manifold, but are rarely reported back to the ordering physician. This article reviews the basic concepts to correctly identify irregularities, monoclonal and oligoclonal peaks by capillary electrophoresis. It then focuses on detecting and reporting typical non-monoclonal irregularities according to their electrophoresis fractions as well as their possible clinical implications.
Subject(s)
Blood Protein Electrophoresis/methods , Blood Proteins/analysis , Electrophoresis, Capillary/methods , Adolescent , Adult , Aged , Aged, 80 and over , Humans , Limit of Detection , Middle Aged , Young AdultABSTRACT
Saccharomyces cerevisiae Rad17p is necessary for cell cycle checkpoint arrests in response to DNA damage. Its known interactions with the checkpoint proteins Mec3p and Ddc1p in a PCNA-like complex indicate a sensor role in damage recognition. In a novel application of the yeast two-hybrid system and by immunoprecipitation, we show here that Rad17p is capable of increased self-interaction following DNA damage introduced by 4-nitroquinoline-N-oxide, camptothecin or partial inactivation of DNA ligase I. Despite overlap of regions required for Rad17p interactions with Rad17p or Mec3p, single amino acid substitutions revealed that Rad17p x Rad17p complex formation is independent of Mec3p. E128K (rad17-1) was found to inhibit Rad17p interaction with Mec3p but not with Rad17p. On the other hand, Phe-121 is essential for Rad17p self-interaction, and its function in checkpoint arrest but not for Mec3p interaction. These differential effects indicate that Rad17p-Rad17p interaction plays a role that is independent of the Rad17p x Mec3p x Ddc1p complex, although our results are also compatible with Rad17p-mediated supercomplex formation of the Rad17p x Mec3p x Ddc1p heterotrimer in response to DNA damage.
Subject(s)
Cell Cycle Proteins/genetics , DNA Damage , Amino Acid Sequence , DNA, Fungal/genetics , DNA-Binding Proteins , Gene Expression Regulation, Fungal , Molecular Sequence Data , Nuclear Proteins , Saccharomyces cerevisiae , Saccharomyces cerevisiae Proteins , Sequence AlignmentABSTRACT
The measurement of urinary marker proteins is not a generally accepted laboratory practice because the results are difficult to interpret. MDI-LABLINK is software for classifying patterns of specific urinary marker proteins. The interpretations are completely user definable thanks to a specific 'pattern definition database'. Our interpretation set is based on Hofmann and Guder's work in measuring and interpreting single urinary proteins. We include additional marker proteins in order to adapt Boesken's SDS-PAGE classification. During the last 3 years, 1905 patterns were fully differentiated and identically interpreted. Firstly, the samples were classified into three patterns: normal (25.8%), predominantly glomerular (27.2%, selective, unselective, mixed, and with additional tubular proteins) and predominantly tubular (36.9%, complete/incomplete form, with additional glomerular proteins); 8.9% showed postrenal proteinuria. Secondly, glomerular selectivity measured by using urinary transferrin/IgG ratio alone correlates well with the established SI index (the ratio between IgG and transferrin clearances). Thirdly, the creatinine concentration substantiates the validity of the sample. The quality of the preanalytical phase can be improved through the ongoing education of the medical staff. Finally, measurement of urinary albumin and alpha-1-microglobulin is mandatory where kidney disease is suspected, has to be ruled out, or requires close monitoring, even when the total protein concentration is normal.
Subject(s)
Database Management Systems , Urinalysis , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Expert Systems , Female , Hematuria/urine , Humans , Male , Middle Aged , Proteinuria/urineABSTRACT
Chk1 is an evolutionarily conserved protein kinase that plays an essential role in mediating G2 arrest in response to DNA damage in Schizosaccharomyces pombe and human cells. It functions by maintaining the inhibition (by phosphorylation of a specific tyrosine residue) of the cyclin-dependent kinase Cdc2 that initiates the G2/M transition. Here, we characterize a structural homologue of Chk1 in the budding yeast Saccharomyces cerevisiae. In this organism, G2/M arrest following DNA damage is considered to be independent of tyrosine phosphorylation of the Cdc2 homologue Cdc28. Nevertheless, a partial defect in G2/M-phase arrest following treatment with ionizing radiation, but not UV radiation, is associated with deletion of CHK1. The fact that such an effect remains detectable in cells synchronized with the microtubule inhibitor nocodazole prior to gamma irradiation implies the existence of a CHK1-dependent checkpoint in M phase. We conclude from epistasis analysis that Chk1 participates in the Pds1-dependent subpathway of M-phase arrest. In spite of the partial checkpoint defect of the chk1 mutant, the survival of colony-forming cells is not notably decreased following UV and gamma irradiation. In two-hybrid screens, we identified a heme-binding stress protein (encoded by the yeast ORF YNL234W), a protein involved in genomic silencing (Sas3) and Chk1 itself as interacting partners of Chk1.
Subject(s)
Cell Cycle Proteins , Protein Kinases/metabolism , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/enzymology , Schizosaccharomyces/enzymology , Amino Acid Sequence , Base Sequence , Checkpoint Kinase 1 , Conserved Sequence , DNA Damage , DNA Primers/genetics , Epistasis, Genetic , Fungal Proteins/genetics , Gene Deletion , Genes, Fungal , Humans , Mitosis , Molecular Sequence Data , Nuclear Proteins/genetics , Protein Kinases/genetics , Radiation Tolerance/drug effects , Saccharomyces cerevisiae/cytology , Saccharomyces cerevisiae/genetics , Schizosaccharomyces/cytology , Schizosaccharomyces/genetics , Schizosaccharomyces pombe Proteins , Securin , Sequence Homology, Amino AcidABSTRACT
The dinB gene of Escherichia coli is an SOS-inducible gene of unknown function. Its mode of regulation and the amino acid sequence similarity of the predicted DinB protein to the UmuC protein of E. coli both suggest a role in cellular responses to DNA damage and probably in error-prone repair. Proteins with sequence similarity to DinB have been predicted from genes cloned from various prokaryotic and eukaryotic organisms, including Caenorhabditis elegans. Here we present the phenotypic characterization of a haploid Saccharomyces cerevisiae strain deleted for the ORF YDR419W, encoding a yeast DinB homolog. The deletion mutant is viable but is moderately sensitive to killing following exposure to ultraviolet (UV) radiation. Hence, we have named the gene RAD30. Steady-state levels of RAD30 transcripts are increased following UV irradiation. UV-induced locus-specific reversion of an ochre allele (arg4-17) is reduced in the rad30 deletion mutant. However, enhanced mutability was observed following treatment with the alkylating agent methylmethanesulfonate (MMS). Spontaneous mutability was also slightly increased. We conclude that RAD30 encodes an accessory function involved in DNA repair and mutagenesis. We speculate that the relatively weak phenotype and the opposite effects on mutability as a function of the type of DNA damage involved may derive from a functional redundancy of yeast proteins which facilitate replicative bypass of non-coding DNA lesions.
Subject(s)
DNA-Directed DNA Polymerase , Escherichia coli Proteins , Fungal Proteins/genetics , Genes, Fungal/genetics , Saccharomyces cerevisiae/genetics , Amino Acid Sequence , Bacterial Proteins/genetics , Base Sequence , DNA Repair , Escherichia coli/genetics , Gene Deletion , Gene Expression Regulation, Fungal , Genes, Fungal/physiology , Molecular Sequence Data , Mutagenesis , Radiation Tolerance , Saccharomyces cerevisiae/radiation effects , Sequence Alignment , Sequence Homology, Amino Acid , Ultraviolet Rays , DNA Polymerase iotaABSTRACT
The Saccharomyces cerevisiae gene MEC1 represents a structural homolog of the human gene ATM mutated in ataxia telangiectasia patients. Like human ataxia telangiectasia cell lines, mec1 mutants are defective in G2 and S-phase cell cycle checkpoints in response to radiation treatment. Here we show an additional defect in G1 arrest following treatment with UV light or gamma rays and map a defective arrest stage at or upstream of START in the yeast cell cycle.
Subject(s)
Cell Cycle/genetics , Fungal Proteins/metabolism , Genes, Fungal , Protein Serine-Threonine Kinases , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/radiation effects , Ataxia Telangiectasia/genetics , Ataxia Telangiectasia Mutated Proteins , Cell Cycle Proteins , DNA-Binding Proteins , Dose-Response Relationship, Radiation , Humans , Intracellular Signaling Peptides and Proteins , Mating Factor , Nocodazole/pharmacology , Peptides/pharmacology , Proteins/genetics , Saccharomyces cerevisiae/cytology , Saccharomyces cerevisiae/drug effects , Sequence Homology , Tumor Suppressor Proteins , Ultraviolet RaysABSTRACT
RAD3 functions in DNA repair and transcription in Saccharomyces cerevisiae and particular rad3 alleles confer a mutator phenotype, possibly as a consequence of defective mismatch correction. We assessed the potential involvement of the Rad3 protein in mismatch correction by comparing heteroduplex repair in isogenic rad3-1 and wild-type strains. The rad3-1 allele increased the spontaneous mutation rate but did not prevent heteroduplex repair or bias its directionality. Instead, the efficiency of mismatch correction was enhanced in the rad3-1 strain. This surprising result prompted us to examine expression of yeast mismatch repair genes. We determined that MSH2, but not MLH1, is transcriptionally regulated during the cell-cycle like PMS1, and that rad3-1 does not increase the transcript levels for these genes in log phase cells. These observations suggest that the rad3-1 mutation gives rise to an enhanced efficiency of mismatch correction via a process that does not involve transcriptional regulation of mismatch repair. Interestingly, mismatch repair also was more efficient when error-editing by yeast DNA polymerase delta was eliminated. We discuss our results in relation to possible mechanisms that may link the rad3-1 mutation to mismatch correction efficiency.
Subject(s)
Adenosine Triphosphatases/genetics , DNA Helicases/genetics , DNA Repair , DNA, Fungal , Saccharomyces cerevisiae/genetics , Base Sequence , Molecular Sequence Data , Mutagenesis , Nucleic Acid Heteroduplexes , Saccharomyces cerevisiae Proteins , Transcription, GeneticABSTRACT
Mutants of the yeast Saccharomyces cerevisiae defective in the RAD17 gene are sensitive to ultraviolet (UV) and gamma radiation and manifest a defect in G2 arrest following radiation treatment. We have cloned the RAD17 gene by complementation of the UV sensitivity of a rad17-1 mutant and identified an ORF of 1.2 kb encoding a predicted gene product of 45.4 kDa with homology to the Schizosaccharomyces pombe rad1+ gene product and to Ustilago maydis Rec1, a known 3'->5'exonuclease. The RAD17 transcript is cell cycle regulated, with maximum steady-state levels during late G1. The rad17-1 mutation represents a missense mutation that maps to a conserved region of the gene. A rad17 disruption mutant grows normally and manifests levels of UV sensitivity similar that of the rad17-1 strain. As previously observed with other genes involved in G2 arrest (such as RAD9 and RAD24), RAD17 regulates radiation-induced G1 checkpoints at at least two possible arrest stages. One is equivalent to or upstream of START, the other at or downstream of the Cdc4 execution point. However, the temperature sensitivity of the cell cycle mutant dna1-1 (a G1 arrest mutant) is not influenced by inactivation of RAD17.
Subject(s)
Cell Cycle Proteins , Cell Cycle/genetics , DNA Damage/genetics , Fungal Proteins/genetics , Saccharomyces cerevisiae/genetics , Amino Acid Sequence , Cloning, Molecular , DNA, Fungal/analysis , DNA-Binding Proteins , Fungal Proteins/chemistry , Fungal Proteins/physiology , Gamma Rays , Genes, Fungal/genetics , Genetic Complementation Test , Molecular Sequence Data , Molecular Weight , Mutation , Nuclear Proteins , Open Reading Frames/genetics , RNA, Fungal/biosynthesis , RNA, Messenger/biosynthesis , Restriction Mapping , Saccharomyces cerevisiae/cytology , Saccharomyces cerevisiae/radiation effects , Saccharomyces cerevisiae Proteins , Sequence Homology, Amino Acid , Temperature , Ultraviolet RaysABSTRACT
Physicians have to confront an enormous output of laboratory data. Normally, only a few experts manually perform the interpretation of highly specialised laboratory tests. PC Windows based 'MDI-LabLink' automates interpretation and expands traditional rule-based expert systems with flexibility and graphic illustrations of complex laboratory data. The laboratory can adjust the interpretation database to its specific needs, maintaining full control over program output. The structured input that 'MDI-LabLink' requires, supports dynamic test scheduling. It can be used to train personnel in the interpretation of laboratory test results. The program provides the clinician with a report that visualises the defect of the evaluated organ system with bitmap pictures and 3-dimensional graphics. Patient follow-ups present in tabular and graphical form. Changes in the severity of the pathobiochemical defect, induced, for example, by therapy, are monitored automatically. Applications available include interpretations of isoenzyme patterns, diagnosis of urinary proteinuria and cerebrospinal fluid analysis.
Subject(s)
Chemistry, Clinical/methods , Clinical Laboratory Information Systems , Data Interpretation, Statistical , Data Display , Follow-Up Studies , Humans , Isoenzymes , L-Lactate Dehydrogenase/analysis , Longitudinal Studies , Reference ValuesABSTRACT
The interstrand cross-link repair gene SNM1 of Saccharomyces cerevisiae was examined for regulation in response to DNA-damaging agents. Induction of SNM1-lacZ fusions was detected in response to nitrogen mustard, cis-platinum (II) diamine dichloride, UV light, and 8-methoxypsoralen + UVA, but not after heat-shock treatment or incubation with 2-dimethylaminoethylchloride, methylmethane sulfonate or 4-nitroquinoline-N-oxide. The promoter of SNM1 contains a 15 bp motif, which shows homology to the DRE2 box of the RAD2 promoter. Similar motifs have been found in promoter regions of other damage-inducible DNA repair genes. Deletion of this motif results in loss of inducibility of SNM1. Also, a putative negative upstream regulation sequence was found to be responsible for repression of constitutive transcription of SNM1. Surprisingly, no inducibility of SNM1 was found after treatment with DNA-damaging agents in strains without an intact DUN1 gene, while regulation seems unchanged in sad1 mutants.
Subject(s)
DNA Damage , DNA Repair/genetics , DNA-Binding Proteins/genetics , Fungal Proteins/genetics , Gene Expression Regulation, Fungal , Nuclear Proteins , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/genetics , Antineoplastic Agents/pharmacology , Base Sequence , Cisplatin/pharmacology , Consensus Sequence/genetics , Cross-Linking Reagents/chemistry , Cross-Linking Reagents/pharmacology , DNA, Fungal/drug effects , DNA, Fungal/radiation effects , Endodeoxyribonucleases , Methoxsalen/pharmacology , Molecular Sequence Data , Mutagenesis/genetics , Promoter Regions, Genetic/genetics , Recombinant Fusion Proteins , Sequence Deletion , Sequence Homology, Nucleic Acid , TATA Box/genetics , Ultraviolet Rays , beta-Galactosidase/genetics , beta-Galactosidase/metabolismABSTRACT
Mutants of the RAD9 gene of Saccharomyces cerevisiae are defective in cell cycle checkpoint arrest in G1 and G2 after treatment with DNA-damaging agents. It is demonstrated that the pronounced G1 arrest observed in yeast after hyperthermia treatment or exposure to paraquat-generated superoxide radicals does not depend on a functional RAD9 gene. For both types of treatments, the mechanism of cell cycle arrest must be considered different from the activation of the DNA damage checkpoint response.
Subject(s)
DNA Damage , G1 Phase/drug effects , Genes, Fungal , Hot Temperature , Paraquat/toxicity , Saccharomyces cerevisiae/drug effects , Saccharomyces cerevisiae/geneticsSubject(s)
DNA Damage/physiology , DNA Repair/physiology , Animals , Cell Transformation, Neoplastic , DNA Damage/genetics , DNA Repair/genetics , DNA Replication , DNA-Binding Proteins/metabolism , Escherichia coli/genetics , Humans , Leukemia, Myeloid, Acute/genetics , Mutagenesis , Recombination, Genetic , Saccharomyces cerevisiae/genetics , Transcription, Genetic , Xeroderma Pigmentosum/geneticsABSTRACT
In mammalian cells, all subunits of the DNA-dependent protein kinase (DNA-PK) have been implicated in the repair of DNA double-strand breaks and in V(D)J recombination. In the yeast Saccharomyces cerevisiae, we have examined the phenotype conferred by a deletion of HDF1, the putative homologue of the 70-kD subunit of the DNA-end binding Ku complex of DNA-PK. The yeast gene does not play a role in radiation-induced cell cycle checkpoint arrest in G1 and G2 or in hydroxyurea-induced checkpoint arrest in S. In cells competent for homologous recombination, we could not detect any sensitivity to ionizing radiation or to methyl methanesulfonate (MMS) conferred by a hdf1 deletion and indeed, the repair of DNA double-strand breaks was not impaired. However, if homologous recombination was disabled (rad52 mutant background), inactivation of HDF1 results in additional sensitization toward ionizing radiation and MMS. These results give further support to the notion that, in contrast to higher eukaryotic cells, homologous recombination is the favored pathway of double-strand break repair in yeast whereas other competing mechanisms such as the suggested pathway of DNA-PK-dependent direct break rejoining are only of minor importance.
Subject(s)
Antigens, Nuclear , DNA Helicases , DNA-Binding Proteins/genetics , Nuclear Proteins/genetics , Recombination, Genetic , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/immunology , Cell Cycle/genetics , DNA Repair , Diploidy , Gene Deletion , Genes, Fungal , Haploidy , Ku Autoantigen , Phenotype , Radiation Tolerance/genetics , Saccharomyces cerevisiae/radiation effectsSubject(s)
Cell Cycle , DNA Damage , Saccharomyces cerevisiae/cytology , Schizosaccharomyces/cytology , DNA Replication , DNA-Directed DNA Polymerase/metabolism , Fungal Proteins/metabolism , Gene Expression Regulation, Fungal , Ribonucleotide Reductases/metabolism , Saccharomyces cerevisiae/genetics , Schizosaccharomyces/genetics , Transcription Factors/metabolismABSTRACT
We have constructed a strain of Saccharomyces cerevisiae with a deletion of the YKL510 open reading frame, which was initially identified in chromosome XI as a homolog of the RAD2 nucleotide excision repair gene (A. Jacquier, P. Legrain, and B. Dujon, Yeast 8:121-132, 1992). The mutant strain exhibits increased sensitivity to UV light and to the alkylating agent methylmethane sulfonate but not to ionizing radiation. We have renamed the YKL510 open reading frame the RAD27 gene, in keeping with the accepted nomenclature for radiation-sensitive yeast mutants. Epistasis analysis indicates that the gene is in the RAD6 group of genes, which are involved in DNA damage tolerance. The mutant strain also exhibits increased plasmid loss, increased spontaneous mutagenesis, and a temperature-sensitive lethality whose phenotype suggests a defect in DNA replication. Levels of the RAD27 gene transcript are cell cycle regulated in a manner similar to those for several other genes whose products are known to be involved in DNA replication. We discuss the possible role of Rad27 protein in DNA repair and replication.
Subject(s)
DNA Repair/genetics , Genes, Fungal , Protein Kinases/genetics , Saccharomyces cerevisiae/genetics , Base Sequence , Cell Cycle/genetics , Checkpoint Kinase 1 , Gene Deletion , Gene Expression Regulation, Enzymologic , Gene Expression Regulation, Fungal , Molecular Sequence Data , Open Reading Frames , Saccharomyces cerevisiae/growth & development , Transcription, GeneticABSTRACT
The delay of S-phase following treatment of yeast cells with DNA-damaging agents is an actively regulated response that requires functional RAD9 and RAD24 genes. An analysis of cell cycle arrest indicates the existence of (at least) two checkpoints for damaged DNA prior to S-phase; one at START (a G1 checkpoint characterized by pheromone sensitivity of arrested cells) and one between the CDC4- and CDC7-mediated steps (termed the G1/S checkpoint). When a dna1-1 mutant (that affects early events of replicon initiation) also carries a rad9 deletion mutation, it manifests a failure to arrest in G1/S following incubation at the restrictive temperature. This failure to execute regulated G1/S arrest is correlated with enhanced thermosensitivity of colony-forming ability. In an attempt to characterize the signal for RAD9 gene-dependent G1 and G1/S cell cycle arrest, we examined the influence of the continued presence of unexcised photoproducts. In mutants defective in nucleotide excision repair, cessation of S-phase was observed at much lower doses of UV radiation compared to excision-proficient cells. However, this response was not RAD9-dependent. We suggest that an intermediate of nucleotide excision repair, such as DNA strand breaks or single-stranded DNA tracts, is required to activate RAD9-dependent G1 and G1/S checkpoint controls.
Subject(s)
Cell Cycle Proteins , Cell Cycle/radiation effects , DNA Damage , DNA-Binding Proteins , Endodeoxyribonucleases , Peptides , Saccharomyces cerevisiae Proteins , Saccharomyces cerevisiae/radiation effects , Cell Cycle/genetics , Flow Cytometry , Fungal Proteins/genetics , Fungal Proteins/metabolism , G1 Phase/genetics , G1 Phase/radiation effects , Gene Deletion , Genes, Fungal , Genotype , Intracellular Signaling Peptides and Proteins , Mating Factor , Peptide Biosynthesis , Pheromones/biosynthesis , Restriction Mapping , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/growth & development , Species Specificity , Transcription, GeneticABSTRACT
Inhibition of DNA synthesis prevents mitotic entry through the action of the S-phase checkpoint. We have isolated S-phase arrest-defective (sad) mutants that show lethality in the presence of the DNA synthesis inhibitor hydroxyurea (HU). Several of these mutants show phenotypes consistent with inappropriate mitotic entry in the presence of unreplicated DNA, indicating a defect in the S-phase checkpoint. sad1 mutants are additionally defective for the G1 and G2 DNA damage checkpoints, and for DNA damage-induced transcription of RNR2 and RNR3. The transcriptional response to DNA damage requires activation of the Dun1 protein kinase. Activation of Dun1 in response to replication blocks or DNA damage is blocked in sad1 mutants. The HU sensitivity of sad1 mutants is suppressed by mutations in CKS1, a subunit of the p34CDC28 kinase, further establishing a link between cell cycle progression and lethality. sad1 mutants are allelic to rad53, a radiation-sensitive mutant. SAD1 encodes an essential protein kinase. The observation that SAD1 controls three distinct checkpoints suggests a common mechanism for cell cycle arrest at these points. Together, these observations implicate protein phosphorylation in the cellular response to DNA damage and replication blocks.
Subject(s)
Protein Kinases/metabolism , Saccharomyces cerevisiae/metabolism , Base Sequence , Cell Cycle/genetics , Cloning, Molecular , DNA Damage , DNA Primers/genetics , DNA Replication/drug effects , DNA, Fungal/genetics , Feedback , Gene Expression Regulation, Fungal , Genes, Fungal , Hydroxyurea/pharmacology , Models, Genetic , Molecular Sequence Data , Mutation , Protein Kinases/genetics , Saccharomyces cerevisiae/cytology , Saccharomyces cerevisiae/genetics , Transcription, GeneticABSTRACT
A newly characterized rad1 missense mutation (rad1-20) in the yeast Saccharomyces cerevisiae maps to a region of the Rad1 polypeptide known to be required for Rad1-Rad10 complex formation. The UV sensitivity of the rad1-20 mutant can be partially and specifically corrected by overexpression of wild-type Rad10 protein. These results suggest that complex formation between the Rad1 and Rad10 proteins is required for nucleotide excision repair.