ABSTRACT
The aim of this study was to evaluate the effects of single oral doses of D-005 (a lipid extract obtained from the fruit oil of Acrocomia crispa) on LPS-induced acute lung injury (ALI) in mice. D-005 batch composition was: lauric (35.8%), oleic (28.4%), myristic (14.2%), palmitic (8.9%), stearic (3.3%), capric (1.9%), caprylic (1.2%), and palmitoleic (0.05%) acids, for a total content of fatty acids of 93.7%. D-005 (200 mg/kg) significantly reduced lung edema (LE) (≈ 28% inhibition) and Lung Weight/Body Weight ratio (LW/BW) (75.8% inhibition). D-005 (25, 50, 100 and 200 mg/kg) produced a significant reduction of Histological score (59.9, 56.1, 53.5 and 73.3% inhibition, respectively). Dexamethasone, as the reference drug, was effective in this experimental model. In conclusion, pretreatment with single oral doses of D-005 significantly prevented the LPS-induced ALI in mice.
El objetivo de este estudio fue evaluar los efectos de dosis orales uÌnicas de D-005 (extracto lipiÌdico obtenido del aceite de frutos de Acrocomia crispa) sobre el danÌo pulmonar agudo (DPA) inducido por LPS en ratones. La composicioÌn del lote de D-005 fue: aÌcido laÌurico (35.8%), oleico (28.4%), miriÌstico (14.2%), palmiÌtico (8.9%), esteaÌrico (3.3%), caÌprico (1.9%), capriÌlico (1.2%) y palmitoleico (0.05%), con un contenido total de aÌcidos grasos de 93.7%. D-005 (200 mg/kg) redujo significativamente el edema pulmonar (EP) (≈ 28% de inhibicioÌn) y la relacioÌn peso pulmoÌn/peso corporal (PP/PC) (75.8% de inhibicioÌn). D-005 (25, 50, 100 y 200 mg/kg) produjo una reduccioÌn significativa de la puntuacioÌn histoloÌgica (59.9, 56.1, 53.5 y 73.3% de inhibicioÌn, respectivamente). La dexametasona, faÌrmaco de referencia, fue efectiva en este modelo experimental. En conclusioÌn, el pretratamiento con dosis orales uÌnicas de D-005 previno significativamente el DPA inducido por LPS en ratones.
Subject(s)
Animals , Mice , Plant Extracts/administration & dosage , Arecaceae , Acute Lung Injury/prevention & control , Plant Extracts/chemistry , Lipopolysaccharides/adverse effects , Administration, Oral , Chromatography, Gas , Acute Lung Injury/chemically induced , Fatty Acids/analysis , Fruit , Lung/drug effectsABSTRACT
This study was aimed to investigate whether the a lipid extract from Acrocomia crispa fruits (D-005) inhibits COX and 5-LOX enzyme activities in vitro. This study demonstrates that D-005 inhibits markedly and in a dose dependent manner COX-2 and 5-LOX activities. The dual inhibition of COX-2 and 5-LOX supports further research on the potential anti-inflammatory effect of D-005.
El objetivo de este estudio fue investigar si el extracto lipídico de los frutos de Acrocomia crispa (D-005) inhibe in vitro las actividades de las enzimas COX y 5-LOX. Este estudio demuestra que el D-005 inhibe marcadamente y de manera dosis dependiente las actividades de la COX-2 y 5-LOX. La inhibición dual de la COX-2 y 5-LOX soportan futuras investigaciones sobre el potencial efecto anti-inflamatorio del D-005.
Subject(s)
Animals , Male , Rats , Anti-Inflammatory Agents/pharmacology , Arecaceae/chemistry , Cyclooxygenase Inhibitors/pharmacology , Lipoxygenase Inhibitors/pharmacology , Plant Extracts/pharmacology , Fruit , In Vitro Techniques , Rats, WistarABSTRACT
A gas chromatographic method using a packed column and 1-eicosanol as an internal standard was validated for the determination of the fatty alcohols that compose policosanol in 10 mg film-coated tablets. The alcohols were determined as trimethylsilyl derivatives, prepared with N-methyl-N-trimethylsilyltrifluoroacetamide. The method can detect degradation products with high retention times, without interfering with the peaks of the active principle. Good linearity (correlation coefficient = 0.9992) and accuracy (mean recovery -100.27 +/- 1.66%) were proven over a range of 25-200% of the nominal concentration. Within- and between-day precision at the nominal 100% value met the acceptance criteria (<2%). Ruggedness was examined through an intralaboratory experimental study in which 6 operational changes were made; the changes were found to have no effect on quantitation, repeatability, resolution, and relative retention time. The method is suitable for the quality control process and stability studies of these tablets.
