ABSTRACT
A recommended protocol has been developed for chromosomal aberration and sister-chromatid exchange assays in CHO, V79 and human lymphocyte cultures. The protocol was based on the responses to a detailed questionnaire completed by North-American and European governmental, university, and contract laboratories using these tests. This report identifies those modifications to previously described methods that are used on a regular basis and clarifies confusing or inconsistent practices. These protocols can be modified for use in other types of cells.
Subject(s)
Chromosome Aberrations , Mutagenicity Tests/methods , Research Design , Sister Chromatid Exchange , Animals , Cells, Cultured , Cricetinae , Female , In Vitro Techniques , Lung/cytology , Lymphocytes/cytology , Mice , Mice, Inbred BALB C , Mice, Inbred C3H , Mutagenicity Tests/standards , Ovary/cytologyABSTRACT
The approach used in the survey of current methodologies used in mammalian cell genotoxicity testing (unscheduled DNA synthesis, mutation, cell transformation and cytogenetics testing) is discussed. The recommended protocols, described in the preceding papers, were developed using responses to detailed questionnaires. This summary outlines general observations related to the survey methodology and to the protocols themselves. Also discussed are the qualities of an effective survey; the evolution of a survey and of a protocol; and the contributions and limitations of this study.
